Antioxidant Activities and Inhibitory Effects of Auricularia Auricular and Its Functional Formula Diet Against Vascular Smooth Muscle Cell in Vitro

.
DOI: 10.4236/fns.2011.24038   PDF   HTML     5,764 Downloads   10,066 Views   Citations

Abstract

The functional formula diet AHP, containing polysaccharides from Auricularia auricular, polyphenolic compounds from Hawthorn (Crataegus) and Pueraria radix, has been recently developed as a dietary intervention against dyslipidemia in our previous study. In the present study, its antioxidant activities and protective effects against proliferation of vascular smooth muscle cells (VSMCs) were investigated. AHP possessed the potent radical-scavenging effects against hydroxyl and superoxide radicals, and also the inhibitory effects against peroxidation of low density lipoprotein induced by Cu2+ in vitro. The protective effects of AHP against proliferation of VSMCs were evaluated through the methodology of serum pharmacology. The serum containing AHP significantly inhibited the proliferation of VSMCs induced by oxidized low density lipoprotein in a time- and dose-dependent manner, and also promoted the nitric oxide production of VSMCs. Our study indicated that this functional formula diet would be a potent alternative as a functional diet to prevent atherosclerosis at early stage.

Share and Cite:

Y. Luo, Z. Xiao, Q. Wang, B. Li and B. Li, "Antioxidant Activities and Inhibitory Effects of Auricularia Auricular and Its Functional Formula Diet Against Vascular Smooth Muscle Cell in Vitro," Food and Nutrition Sciences, Vol. 2 No. 4, 2011, pp. 265-271. doi: 10.4236/fns.2011.24038.

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1] C. K. Glass and J. L. Witztum, “Atherosclerosis. The Road Ahead,” Cell, Vol. 104, No. 4, 2001, pp. 503-516. doi:10.1016/S0092-8674(01)00238-0
[2] Y. H. Zhang, J. T. Liu, B. Y. Wen and N. Liu, “Mechanisms of Inhibiting Proliferation of Vascular Smooth Muscle Cells by Serum of Rats Treated with Dahuang Zhechong Pill,” Journal of Ethnopharmacology, Vol. 124, No. 1, 2009, pp. 125-129. doi:10.1016/j.jep.2009.04.012
[3] Y. H. Zhang, J. T. Liu, B. Y. Wen and X. H. Xiao, “In Vitro Inhibition of Proliferation of Vascular Smooth Muscle Cells by Serum of Rats Treated with Dahuang Zhechong Pill,” Journal of Ethnopharmacology, Vol. 112, No. 2, 2007, pp. 375-379. doi:10.1016/j.jep.2007.03.018
[4] D. Giugliano, “Dietary Antioxidants for Cardiovascular Prevention,” Nutrition Metabolism and Cardiovascular Diseases, Vol. 10, No. 1, 2000, pp. 38-44.
[5] H. E. Seifried, D. E. Anderson, E. I. Fisher and J. A. Mil- ner, “A Review of the Interaction among Dietary Antioxidants and Reactive Oxygen Species,” Journal of Nutritional Biochemistry, Vol. 18, No. 9, 2007, pp. 567-579. doi:10.1016/j.jnutbio.2006.10.007
[6] F. Shahidi, “Functional Foods: Their Role in Health Promotion and Disease Prevention,” Journal of Food Science, Vol. 69, No. 5, 2004, pp. 146-149. doi:10.1111/j.1365-2621.2004.tb10727.x
[7] Y. Luo, G. Chen, B. Li, B. Ji, Z. Xiao and F. Tian, “Dietary Intervention with AHP, a Functional Formula Diet, Improves Both Serum and Hepatic Lipids Profile in Dyslipidemia Mice,” Journal Of Food Science, Vol. 74, No. 6, 2009, pp. H189-H195. doi:10.1111/j.1750-3841.2009.01241.x
[8] Y. Luo, G. Chen, B. Li, B. Ji, Y. Guo and F. Tian, “Evaluation of Antioxidative and Hypolipidemic Properties of a Novel Functional Diet Formulation of Auricularia Auricula and Hawthorn,” Innovative Food Science and Em- erging Technologies, Vol. 10, No. 2, 2009, pp. 215-221. doi:10.1016/j.ifset.2008.06.004
[9] G. Chen, Y. Luo, B. Ji, Y. Li, Y. Guo, W. S and Z. Xiao, “Effect of Polysaccharide from Auricularia Auricula on Blood Lipid Metabolism and Lipoprotein Lipase Activity of ICR Mice Fed a Cholesterol-Enriched Diet,” Journal of Food Science, Vol. 73, No. 6, 2008, pp. H103-H108. doi:10.1111/j.1750-3841.2008.00821.x
[10] I. G. De Avellar, M. M. Magalhaes, A. B. Silva, L. L. Souza, A. C. Leitao and M. Hermes-Lima, “Reevaluating the Role of 1,10-Phenanthroline in Oxidative Reactions Involving Ferrous Ions and Dna Damage,” Biochimica et Biophysica Acta, Vol. 1675, No. 1-3, 2004, pp. 46-53.
[11] R. C. Stewar and J. D. Beewley, “Lipid Peroxidation Associated with Accelerated Aging of Soybean Areas,” Plant Physiology, Vol. 65, No. 2, 1980, pp. 245-248. doi:10.1104/pp.65.2.245
[12] L. K. Leung, Y. L. Su, R. Y. Chen, Z. S. Zhang, Y. Huang and Z. Y. Chen, “Theaflavins in Black Tea and Catechins in Green Tea are Equally Effective Antioxidants,” Journal of Nutrition, Vol. 131, No. 9, 2001, pp. 2248-2251.
[13] H. M. Liu, Y. Cheng, J. F. Lu and R. C. Li, “The Mechanism of Kinetic Inhibition of Cu (Ii)-Induced Oxidation of Low Density Lipoprotein by Lanthanide Ions,” Journal of Inorganic Biochemistry, Vol. 100, No. 7, 2006, pp. 1280- 1289. doi:10.1016/j.jinorgbio.2006.03.002
[14] M. N. Chahine, D. P. Blackwood, E. Dibrov, M. N. Richard and G. N. Pierce, “Oxidized LDL Affects Smooth Muscle Cell Growth through MAPK-Mediated Actions on Nuclear Protein Import,” Journal of Molecular and Cellular Cardiology, Vol. 46, No. 3, 2009, pp. 431-441. doi:10.1016/j.yjmcc.2008.10.009
[15] E. Niki, “Assessment of Antioxidant Capacity in Vitro and in Vivo,” Free Radical Biology and Medicine, Vol. 49, No. 4, 2010, pp. 503-515. doi:10.1016/j.freeradbiomed.2010.04.016
[16] Y. S. Kho, S. Vikineswary, N. Abdulah, U. R. Kuppusamy and H. I. Oh, “Antioxidant Capacity of Fresh and Proce- ssed Fruit Bodies and Mycelium of Auricularia Auricula-Judae (Fr.) Quél,” Journal of Medicinal Food, Vol. 12, No. 1, 2009, pp. 167-174. doi:10.1089/jmf.2007.0568
[17] Q. Wu, Z. Tan, H. Liu, L. Gao, S. Wu, J. Luo, W. Zhang, T. Zhao, J. Yu and X. Xu, “Chemical Characterization of Auricularia Auricula Polysaccharides and Its Pharmacological Effect on Heart Antioxidant Enzyme Activities and Left Ventricular Function in Aged Mice,” International Journal of Biological Macromolecules, Vol. 46, No. 3, 2010, pp. 284-288. doi:10.1016/j.ijbiomac.2010.01.016
[18] H. Zhang, Z. Y. Wang, Z. Zhang and X. Wang, “Purified Auricularia Auricular-Judae Polysaccharide (AAP I-a) Prevents Oxidative Stress in an Ageing Mouse Model,” Carbohydrate Polymers, Vol. 84, No. 1, 2011, pp. 638- 648. doi:10.1016/j.carbpol.2010.12.044
[19] L. S. Fan, S. H. Zhang, L. Yu and L. Ma, “Evaluation of Antioxidant Property and Quality of Breads Containing Auricularia Auricula Polysaccharide Flour,” Food Chemistry, Vol. 101, No. 3, 2006, pp. 1158-1163. doi:10.1016/j.foodchem.2006.03.017
[20] J. L. Liu, J. F. Yuan and Z. Q. Zhang, “Microwave-As- sisted Extraction Optimised with Response Surface Methodology and Antioxidant Activity of Polyphenols from Hawthorn (Crataegus Pinnatifida Bge.) Fruit,” Internati- onal Journal of Food Science and Technology, Vol. 45, No. 11, 2010, pp. 2400-2406. doi:10.1111/j.1365-2621.2010.02416.x
[21] W. Cherdshewasart and W. Sutjit, “Correlation of Antioxidant Activity and Major Isoflavonoid Contents of the Phytoestrogen-Rich Pueraria Mirifica and Pueraria Lobata Tubers,” Phytomedicine, Vol. 15, No. 1-2, 2008, pp. 38-43. doi:10.1016/j.phymed.2007.07.058
[22] R. Stocker and J. F. Keaney, “Role of Oxidative Modifications in Atherosclerosis,” Physiological Review, Vol. 84, No. 4, 2004, pp. 1381-1478. doi:10.1152/physrev.00047.2003
[23] D. Steinberg, “Low Density Lipoprotein Oxidation and Its Pathobiological Significance,” Journal of Biological Che- mistry, Vol. 272, No. 34, 1997, pp. 20963-20966. doi:10.1074/jbc.272.34.20963
[24] H. Esterbauer and P. Ramos, “Chemistry and Pathophysiology of Oxidation of LDL,” Review of Physiology Biochemistry Pharmacology, Vol. 127, 1995, pp. 31-64. doi:10.1007/BFb0048264
[25] A. C. Kaliora, G. V. Z. Dedoussis and H. L. Schmidt, “Di- etary Antioxidant in Preventing Atherogenesis,” Atherosclerosis, Vol. 187, No. 1, 2006, pp. 1-17. doi:10.1016/j.atherosclerosis.2005.11.001
[26] Z. S. Zhang, Q. Chang, M. Zhu, Y. Huang, W. K. K. Ho and Z. Y. Chen, “Characterization of Antioxidants Present in Hawthorn Fruits,” Journal of Nutritional Biochemistry, Vol. 12, No. 3, 2001, pp. 144-152. doi:10.1016/S0955-2863(00)00137-6
[27] S. Tashino, “‘Serum Pharmacology’ and ‘Serum Pharmaceutical Chemistry’: From Pharmacology of Chinese Traditional Medicines to Start a New Measurement of Drug Concentration in Blood,” Therapeutic Drug Monitoring Research, Vol. 5, 1988, pp. 54-64.
[28] D. Tang, Z. Zhang, Y. Gao, Y. Wei and L. Han, “Protective Effects of Serum Containing Ginkgo Biloba Extract on Glomerulosclerosis in Rat Mesangial Cells,” Journal of Ethnopharmacology, Vol. 124, No. 1, 2009, pp. 26-33. doi:10.1016/j.jep.2009.04.017
[29] H. Zhang, B. Ji, G. Chen, F. Zhou, Y. Luo, H. Yu, F. Gao, Z. Zhang and H. Li, “A Combination of Grape Seed-De- rived Procyanidins and Gypenosides Alleviates Insulin Resistance in Mice and HepG2 Cells,” Journal of Food Science, Vol. 74, No. 1, 2009, pp. H1-H7. doi:10.1111/j.1750-3841.2008.00976.x
[30] C. A. Schmitt and V. M. Dirsch, “Modulation of Endothelial Nitric Oxide by Plant-Derived Products,” Nitric Oxide, Vol. 21, No. 2, 2009, pp. 77-91. doi:10.1016/j.niox.2009.05.006
[31] W. Bochu, Z. Liancai and C. Qi, “Primary Study on the Application of Serum Pharmacology in Chinese Traditional Medicine,” Colloids and Surfaces B: Biointerfaces, Vol. 43, No. 3-4, 2005, pp. 194-197. doi:10.1016/j.colsurfb.2005.04.013
[32] K. Acharya, K. Samui, M. Rai, B. B. Dutta and R. Ach- arya, “Antioxidant and Nitric Oxide Synthase Activation Properties of Auricularia Auricular,” Indian Journal of Experimental Biology, Vol. 42, No. 5, 2004, pp. 538-540.
[33] G. Chen, Y. Luo, B. Ji, B. Li, W. Su, Z. Xiao and G. Zhang, “Hypocholesterolemic Effects of Auricularia Auricula Ethanol Extract in ICR Mice Fed a Cholesterol-Enriched Diet,” 2010. http://www.springerlink.com/content/b156t26171935104/

  
comments powered by Disqus

Copyright © 2020 by authors and Scientific Research Publishing Inc.

Creative Commons License

This work and the related PDF file are licensed under a Creative Commons Attribution 4.0 International License.