Effect of Aspergillus awamori-Fermented Burdock Root on Mouse Diabetes Induced by Alloxan—Prevention of Cell Apoptosis


Root of burdock contains high amounts of dietary fibers and polyphenols. To improve the functional properties, the root was fermented with Aspergillus awamori. Effect of the fermented burdock on alloxan-induced mouse diabetes was examined. A diet containing the 5% fermented burdock powers was prepared to examine effect of the burdock diet on alloxan-induced mouse diabetes. Mice fed the burdock diet and the control diet for 14 weeks. Then, alloxan (200 mg/kg of body weight) was administrated to each mouse. After 5 days from the administration, blood glucose assay and glucose tolerance test were carried out. Incidence of hyperglycemia decreased and the glucose metabolism was improved when mice fed the burdock diet. Insulin, C-peptide, biomarkers of oxidative stress in plasma and apoptosis in pancreas were examined and compared to those obtained from mice fed the control diet. It is deduced that alloxan-induced diabetes is caused to lower insulin concentration. The fermented-burdock diet improves the diabetes and prevents apoptosis in the pancreas.


Share and Cite:

Takemoto, K. , Doi, W. , Zukeran, A. , Inoue, J. , Ishihara, K. and Masuoka, N. (2014) Effect of Aspergillus awamori-Fermented Burdock Root on Mouse Diabetes Induced by Alloxan—Prevention of Cell Apoptosis. Food and Nutrition Sciences, 5, 1554-1560. doi: 10.4236/fns.2014.516168.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] Wang, D.H., Masuoka, N. and Kira, S. (2003) Animal Model for Oxidative Stress Research—Catalase Mutant Mice. Environmental Health and Preventive Medicine, 8, 37-40.
[2] Szkudelski, T (2001) The Mechanism of Alloxan and Streptozotocin Action in B Cells of the Rat Pancreas. Physiological Research, 50, 537-546.
[3] Takemoto, K., Tanaka, M., Iwata, H., Nishihara, R., Ishihara, K., Wang, D.H., Ogino, K., Taniuchi, K. and Masuoka, N. (2009) Low Catalase Activity in Blood Is Associated with the Diabetes Caused by Alloxan. Clinica Chimica Acta, 407, 43-46.
[4] Kikumoto, Y., Sugiyama, H., Inoue, T., Morinaga, H., Takiue, K., Kitagawa, M., Fukuoka, N., Saeki, M., Maeshima, Y., Wang, D.H., Ogino, K., Masuoka, N. and Makino, H. (2010) Sensitization to Alloxan-Induced Diabetes and Pancreatic Cell Apotosis in Acatalasemic Mice. Biochimica et Biophysica Acta, 1802, 240-246.
[5] Maruta, Y., Kawabata, J. and Niki, R. (1995) Antioxidative Caffeoylquinic Acid Derivatives in the Roots of Burdock (Arctium lappa L.). Journal of Agricultural and Food Chemistry, 43, 2592-2595.
[6] Lin, L.-Z. and Harnly, J.M. (2008) Identification of Hydroxycinnamoylquinic Acids of Arnica Flowers and Burdock Roots Using a Standardized LC-DAD-ESI/MS Profiling Method. Journal of Agricultural and Food Chemistry, 56, 10105-10114.
[7] Okazaki, Y., Sitanggang, N.V., Sato, S., Ohnishi, N., Inoue, J., Iguchi, T., Watanabe, T., Tomotake, H., Harada, K. and Kato, N. (2013) Burdock Fermented by Aspergillus awamori Elevates Cecum Bifidobacterium, and Reduces Fecal Deoxycholic Acid and Adipose Tissue Weight in Rats Fed a High-Fat Diet. Bioscience, Biotechnology, and Biochemistry, 77, 53-57.
[8] Kamimura, W., Doi, W., Takemoto, K., Ishihara, K., Wang, D.H., Sugiyama, H., Oda, S. and Masuoka, N. (2013) Effect of Vitamin E on Alloxan-Induced Mouse Diabetes. Clinical Biochemistry, 46, 795-798.
[9] Choi, E.J., Bae, S.C., Yu, R., Youn, J. and Sung, M.K. (2009) Dietary Vitamin E and Quercetin Modulate Inflammatory Responses of Collagen-Induced Arthritis in Mice. Journal of Medicinal Food, 12, 770-775.
[10] Feinstein, R.N., Braun, J.T. and Howard, J.B. (1967) Acatalasemic and Hypocathalasemic Mouse Mutants. II. Mutational Variations in Blood and Solid Tissue Catalases. Archives of Biochemistry and Biophysics, 120, 165-169.
[11] Reeves, P.G., Nielson, F.H. and Fahey Jr., G.C. (1993) AIN-93 Purified Diets for Laboratory Rodents: Final Report of the American Institute of Nutrition and Hoc Writing Committee on the Reformulation of the AIN-73A Rodent Diet. Journal of Nutrition, 123, 1939-1951.
[12] Gao, D., Li, Q., Liu, Z., Li, Y., Liu, Z., Fan, Y., Li, K., Han, Z. and Li, J. (2007) Hypoglycemic Effects and Mechanisms of Action of Cortex Lycii radicis on Alloxan-Induced Diabetic Mice. Yakugaku Zasshi, 127, 1715-1721.
[13] Nam, S.Y., Lee, M.K. and Sabapathy, K. (2008) The Tumour-Suppressor p53 Is Not Required for Pancreatic Beta Cell Death during Diabetes and upon Irradiation. Journal of Physiology, 586, 407-417.
[14] Masuoka, N., Wakimoto, M., Ubuka, T. and Nakano, T. (1996) Spectrophotometric Determination of Hydrogen Peroxide: Catalase Activity and Rates of Hydrogen Peroxide Removal by Erythrocytes. Clinica Chimica Acta, 254, 101-112. http://dx.doi.org/10.1016/0009-8981(96)06374-7
[15] Matthews, D.R., Hosker, J.P., Rudenski, A.S., Naylor, B.A., Treacher, D.F. and Turner, R.C. (1985) Homeostasis Model Assessment: Insulin Resistance and β-Cell Function from Fasting Plasma Glucose and Insulin Concentrations in Man. Diabetologia, 28, 412-419.
[16] Zamami, Y., Takatori, S., Goda, M., Koyama, T., Iwatani, Y., Jin, X., Takai-Doi, S. and Kawasaki, H. (2008) Royal Jelly Ameliorates Insulin Resistance in Fructose-Drinking Rats. Biological and Pharmaceutical Bulletin, 31, 2103-2107. http://dx.doi.org/10.1248/bpb.31.2103
[17] Yamamoto, H., Uchigata, Y. and Okamoto, H. (1981) Streptozotocin and Alloxan Induce DNA Strand Breaks and Poly (ADP-Ribose) Synthetase in Pancreatic Islets. Nature, 294, 284-286.

Copyright © 2022 by authors and Scientific Research Publishing Inc.

Creative Commons License

This work and the related PDF file are licensed under a Creative Commons Attribution 4.0 International License.