Extraction and Stability of Anthocyanins Present in the Skin of the Dragon Fruit (Hylocereus undatus)

DOI: 10.4236/fns.2013.412156   PDF   HTML     6,329 Downloads   10,721 Views   Citations

Abstract

The extraction of anthocyanins present in the skin of the dragon fruit was performed using trifluoroacetic acid (TFA) plus a mixture of methanol, acetic acid and water; the anthocyanins were then purified with a LC-18 cartridge, using methanol acidified with TFA as eluent, reaching concentrations of 44.3865 ± 1.3125 mg/100g of sample. The extracts were put through stability tests under different storage conditions, modifying the pH of the extracts (pH of 1, 4 and 6), the temperature (4℃, 25℃ and 68℃) and the absence and presence of light for a time period of 4 days; the tests indiated that anthocyanins remain more stable at a temperature of 4℃ with a pH of 4 in the absence of light, retaining up to 80% of the pigment. Three anthocyanins were partially identified in the extracts by high performance liquid chromatography (HPLC); they were: cyanidin 3-O-glucoside, cyanidin 3,5 O-glucoside and pelargonidin 3,5 O-glucoside.

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M. Vargas, J. Cortez, E. Duch, A. Lizama and C. Méndez, "Extraction and Stability of Anthocyanins Present in the Skin of the Dragon Fruit (Hylocereus undatus)," Food and Nutrition Sciences, Vol. 4 No. 12, 2013, pp. 1221-1228. doi: 10.4236/fns.2013.412156.

Conflicts of Interest

The authors declare no conflicts of interest.

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