The Joint Use of Electronic Nose and Electronic Tongue for the Evaluation of the Sensorial Properties of Green and Black Tea Infusions as Related to Their Chemical Composition


The objectives of the present study were to determine the effects of the brewing method on the amount of the major catechins, methylxanthines, total polyphenols and antioxidant capacity in green and black teas, and to correlate the chemical composition of tea infusions to their sensorial properties by the combined use of the electronic nose and tongue. For this purpose, tea infusions were prepared from 7 green teas and 6 black teas using different extraction times (3 - 5 and 10 min). The extraction of total polyphenols and catechins was increased by prolonging the infusion time from 3 - 5 to 10 min, while the caffeine amounts did not increase. The antioxidant activity of teas was well correlated with polyphenol content. Electronic tongue and electronic nose were able to discriminate green teas from black teas on the basis of their sensorial properties. Considering the taste, green teas were characterized by astringency and aftertaste-astringency and this sensation was increased by prolonging the infusion time; black teas were perceived as more bitter, sour, salty and the extraction time affected the astringent sensation. The aroma of green and black teas was discriminated by specific sensors and the increase of the extraction time produced more richly flavoured infusions. This work adds information about correlation between sensorial properties, antioxidant capacity and chemical composition of green and black teas.

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S. Buratti, A. Casiraghi, P. Minghetti and G. Giovanelli, "The Joint Use of Electronic Nose and Electronic Tongue for the Evaluation of the Sensorial Properties of Green and Black Tea Infusions as Related to Their Chemical Composition," Food and Nutrition Sciences, Vol. 4 No. 6, 2013, pp. 605-615. doi: 10.4236/fns.2013.46078.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] Y. Shishikura and S. Khokhar, “Factors Affecting the Levels of Catechins and Caffeine in Tea Beverage: Estimated Daily Intakes and Antioxidant Activity,” Journal of the Science of Food and Agriculture, Vol. 85, No. 12, 2005, pp. 2125-2133. doi:10.1002/jsfa.2206
[2] D. A. Balentine, S. A. Wiseman and L. C. M. Bouwens, “The Chemistry of Tea Flavonoids,” CRC Critical Reviews in Food Science and Nutrition, Vol. 37, No. 8, 1997, pp. 693-704. doi:10.1080/10408399709527797
[3] C. Lakenbrink, S. Lapczynski, B. Maiwald and U. H. Engelhardt, “Flavonoids and Other Polyphenols in Consumer Brews of Tea and Other Caffeinated Beverages,” Journal of the Science of Food and Agriculture, Vol. 48, No. 7, 2000, pp. 2848-2852. doi:10.1021/jf9908042
[4] C. Astill, M. R. Birch, C. Dacombe, P. G. Humphrey and P. T. Martin, “Factors Affecting the Caffeine and Plyphenol Contents of Black and Green Tea Infusions,” Journal of Agricultural and Food Chemistry, Vol. 49, No. 11, 2001, pp. 5340-5347. doi:10.1021/jf010759+
[5] V. Sharma, A. Gulati and S. D. Ravindranath, “Extractability of Tea Catechins as a Function of Manufacture Procedure and Temperature of Infusion,” Food Chemistry, Vol. 93, No. 1, 2005, pp. 141-148. doi:10.1016/j.foodchem.2004.10.016
[6] V. Sharma, A. Gulati, S. D. Ravindranath and V. Kumar, “A Simple and Convenient Method for Analysis of Tea Biochemicals by Reverse Phase,” HPLC Journal of Food Composition and Analysis, Vol. 18, No. 6, 2005, pp. 583594. doi:10.1016/j.jfca.2004.02.015
[7] D. J. Yang, L. S. Hwang and J. T. Lin, “Effects of Different Steeping Methods and Storage on Caffeine, Catechins and Gallic Acid in Bag Tea Infusions,” Journal of Chromatography A, Vol. 1156, No. 1-2, 2007, pp. 312320. doi:10.1016/j.chroma.2006.11.088
[8] D. Horzic, D. Komes, A. Belscak, K. K. Ganic, D. Ivekovic and D. Karlovic, “The Composition of Polyphenols and Methylxanthines in Teas and Herbal Infusions,” Food Chemistry, Vol. 115, No. 2, 2009, pp. 441-448. doi:10.1016/j.foodchem.2008.12.022
[9] W. J. Cheong, M. H. Park, G. W. Kang, J. H. Ko and Y. J. Seo, “Determination of Catechin Compounds in Korean Green Tea Infusions under Various Extraction Conditions by High Performance Liquid Chromatography,” Bulletin of the Korean Chemical Society, Vol. 26, No. 5, 2005, pp. 747-754. doi:10.5012/bkcs.2005.26.5.747
[10] Perva-Uzunalic, M. Skerget, Z. Knez, B. Weinreich, F. Otto and S. Gruner, “Extraction of Active Ingredients from Green Tea (Camellia Sinensis): Extraction Efficiency of Major Catechins and Caffeine,” Food Chemistry, Vol. 96, No. 4, 2006, pp. 597-605. doi:10.1016/j.foodchem.2005.03.015
[11] S. Scharbert, N. Holzmann and T. Hofmann, “Identification of the Astringent Taste Compounds in Black Tea Infusions by Combining Instrumental Analysis and Human Bioresponse,” Journal of Agricultural and Food Chemistry, Vol. 52, No. 11, 2004, pp. 3498-3508.
[12] H. Yu and J. Wang, “Discrimination of LongJing GreenTea Grade by Electronic Nose,” Sensors and Actuators B, Vol. 122, No. 1, 2007, pp. 134-140. doi:10.1016/j.snb.2006.05.019
[13] B. Tudu, A. Jana, A. Metla, D. Ghosh, N. Bhattacharyya and R. Bandyopadhyay, “Electronic Nose for Black Tea Quality Evaluation by an Incremental RBF Network,” Sensors and Actuators B, Vol. 138, No. 1, 2009, pp. 9095. doi:10.1016/j.snb.2009.02.025
[14] H. Yu, J. Wang, H. Xiao and M. Liu, “Quality Grade Identification of Green Tea Using the Eigenvalues of PCA Based on the E-Nose Signals,” Sensors and Actuators B, Vol. 140, No. 2, 2009, pp. 378-382. doi:10.1016/j.snb.2009.05.008
[15] N. Bhattacharyya, S. Seth, B. Tudu, P. Tamuly, A. Jan, D. Ghosh, R. Bandyopadhyay and M. Bhuyan, “Monitoring of Black Tea Fermentation Process Using Electronic Nose,” Journal of Food Engineering, Vol. 80, No. 4, 2007, pp. 1146-1156. doi:10.1016/j.jfoodeng.2006.09.006
[16] N. Bhattacharyya, B. Tudu, A. Jana, D. Ghosh, R. Bandyopadhyay and M. Bhuyan, “Preemptive Identification of Optimum Fermentation Time for Lack Tea Using Electronic Nose,” Sensors and Actuators B, Vol. 131, No. 1, 2008, pp. 110-116. doi:10.1016/j.snb.2007.12.032
[17] Q. Chen, J. Zhao and S. Vittayapadung, “Identification of Green Tea Grade Level Using Electronic Tongue and Pattern Recognition,” Food Research International, Vol. 41, No. 5, 2008, pp. 500-504. doi:10.1016/j.foodres.2008.03.005
[18] M. Palit, B. Tudu, N. Bhattacharyya, A. Dutta, P. K. Dutta, A. Jana, R. Bandyopadhyay and A. Chatterjee, “Comparison of Multivariate Preprocessing Techniques as Applied to Electronic Tongue Based Pattern Classification for Black Tea,” Analytica Chimica Acta, Vol. 675, No. 1, 2010, pp. 8-15. doi:10.1016/j.aca.2010.06.036
[19] W. He, X. Hu, L. Zhao, X. Liao, Y. Zhang, M. Zhang and J. Wu, “Evaluation of Chinese Tea by Electronic Tongue: Correlation with Sensory Properties and Classification According to Geographical Origin and Grade Level,” Food Research International, Vol. 42, No. 10, 2008, pp. 1462-1467. doi:10.1016/j.foodres.2009.08.008
[20] R. Banerjee, B. Tudu, L. Shaw, A. Jana, N. Bhattacharyya and R. Bandyopadhyay, “Instrumental Testing of Tea by Combining the Responses of Electronic Nose and Tongue,” Journal of Food Engineering, Vol. 110, No. 3, 2012, pp. 356-363. doi:10.1016/j.jfoodeng.2011.12.037
[21] L. Peng, X. H. Song, X. G. Shi, J. Li and C. X. Ye, “An Improved HPLC Method for Simultaneous Determination of Phenolic Compounds, Purine Alkaloids and Theanine in Camellia Species,” Journal of Food Composition and Analysis, Vol. 21, No. 7, 2008, pp. 559-563. doi:10.1016/j.jfca.2008.05.002
[22] V. L. Singleton and J. A. Rossi “Colorimetry of Total Phenolics with Phosphomolybdic-Phosphotungstic Acid Reagents,” American Journal of Enology and Viticulture, Vol. 16, No. 3, 1965, pp. 144-158.
[23] S. Buratti, M. Scampicchio, G. Giovanelli and S. Mannino, “A Low-Cost and Low-Tech Electrochemical Flow System for the Evaluation of Total Phenolic Content and Antioxidant Power of Tea Infusions,” Talanta, Vol. 75, No. 1, 2008, pp. 312-316. doi:10.1016/j.talanta.2007.11.014
[24] Y. Kobayashi, M. Habara, H. Ikezazki, R. Chen, Y. Naito and K. Toko, “Advanced Taste Sensors Based on Artificial Lipids and Global Selectivity to Basic Taste Qualities and High Correlation to Sensory Scores,” Sensors, Vol. 10, No. 4, 2010, pp. 3411-3443. doi:10.3390/s100403411
[25] J. A. B. Baptista, J. F. P. Tavares and R. C. B. Carvalho, “Comparison of Catechins and Aromas among Different Green Teas Using HPLC/SPME-GC,” Food Research International, Vol. 31, No. 10, 1998, pp. 729-736. doi:10.1016/S0963-9969(99)00052-6
[26] P. Pripdeevech and T. Machan, “Fingerprint of Volatile Flavour Constituents and Antioxidant Activities of Teas from Thailand,” Food Chemistry, Vol. 125, No. 2, 2011, pp. 797-802. doi:10.1016/j.foodchem.2010.09.074
[27] L. F. Wang, J. Y. Lee, J. O. Chung, J. H. Baik, S. So and S. K. Park, “Discrimination of Teas with Different Degrees of Fermentation by SPME-GC Analysis of the Characteristic Volatile Flavour Compounds,” Food Chemistry, Vol. 109, No. 1, 2008, pp. 196-206. doi:10.1016/j.foodchem.2007.12.054
[28] K. Kumazawa and H. Masuda, “Identification of Potent Odorants in Japanese Green Tea (Sen-cha),” Journal of Agricultural and Food Chemistry, Vol. 47, No. 12, 1999, pp. 5169-5172. doi:10.1021/jf9906782
[29] K. Kumazawa and H. Masuda, “Identification of Potent Odorants in Different Green Tea Varieties Using Flavour Dilution Technique,” Journal of Agricultural and Food Chemistry, Vol. 50, No. 2, 2002, pp. 5660-5663. doi:10.1021/jf020498j

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