ABNT (Brazilian Association of Technical Norms), “N- BR 12713 - Water Ecotoxicology - Acute Toxicity - Assay with Daphnia ssp. (Cladocera, Crustacea),” Rio de Janeiro, 2004.

has been cited by the following article:

• TITLE: Evaluation of Acute Chromium (III) Toxicity in Relation to Daphnia Similis

  AUTHORS: Petr Melnikov, Tânia C. M. de Freitas

  KEYWORDS: Water pollution, Chromium (III), Daphnia similis, Acute toxicity test

  JOURNAL NAME: Journal of Water Resource and Protection, Vol.3 No.2, February 28, 2011

  ABSTRACT: Acute chromium (III) toxicity in relation to Daphnia similis, most appropriate for Brazilian environment has been evaluated. The preliminary toxicity test showed that the median effective concentration was 10 mg/l. After having performed the final test, the immobility percentage was calculated for each concentration in relation to the total number of the used organisms within the chosen pH range. It was shown that the proposed methodology rendered realistic results and the 48-h CE50 value under the test conditions is 3.24 mg/l. The minimum concentration that did not show any toxicity to Daphnia similis was 2.5 mg of chromium (III) per liter. The obtained level is superior to the maximum limit recommended by the Brazilian norms, 0.5 mg/l.