TITLE:
Error Corrected Sub-Monolayer Ellipsometry for Measurement of Biomolecular Interactions
AUTHORS:
Udo Riss
KEYWORDS:
Ellipsometry; Ellipsometer; Antibody; Antigen; Virus; Membrane; Kinetics; Electrodynamics
JOURNAL NAME:
Open Journal of Biophysics,
Vol.3 No.1A,
February
27,
2013
ABSTRACT:
Already in 1946 Alexandre Rothen from the Rockefeller Institute for
Medical Research, New York published the use of ellipsometry for the
measurement of antigen-antibody interactions. And he found some effects that
could not be explained by the assumption that the interaction of the molecules
is only of chemical nature. 55 years later we started research on
antibody-antigen interaction and found similar results. To make sure that these
measurements are not produced by measurement artifacts, each component of our
measurement technique is error analyzed and error corrected if necessary. With
such type of error corrected instrumentation we found, that there must be an
interaction between antigens and antibodies that is based on longitudinal
electromagnetic waves, which are able to work through thin 7 nm membranes. A
similar interaction is found for the virus-antigen interaction. Our measurement
results are in contrast to the assumption that the antigen-antibody and
antigen-virus interaction is only of chemical nature.