TITLE:
UPLC-QTOF-MS Analysis of Extracts from the Leaves of Pouteria caimito (Sapotaceae) and Their Antioxidant Activity
AUTHORS:
Lorrayne Clemente Ribeiro de Sousa, Almir Ribeiro de Carvalho Junior, Mario Geraldo de Carvalho, Tania Maria Sarmento da Silva, Rafaela Oliveira Ferreira
KEYWORDS:
Abiu, UPLC, Triterpenes, DPPH, Antioxidants
JOURNAL NAME:
Journal of Biosciences and Medicines,
Vol.7 No.3,
March
25,
2019
ABSTRACT: This study describes the phytochemical profile and
antioxidant activity of an extract from the leaves of Pouteria caimito (Sapotaceae). The extract in ethanol was obtained by
maceration at room temperature and subjected to the liquid-liquid partition to
obtain fractions in hexane and ethyl acetate. Steroids, triterpenes, saponins,
alkaloids and flavonoids were identified by the phytochemical prospection of
extracts and fractions from the leaves. The analysis of the ethyl acetate
fraction by UPLC-QTOF-MS allowed us to identify eight triterpenes, namely, euscaphic
acid (1), hyptadienic acid (2), betulinic acid (3), oleanolic acid (4), ursolic
acid (5), 3β-(O-p-coumaroyl)- alphitolic acid (6), 3β-(O-p-coumaroyl)-maslinic
acid (7) and 3β-(O-p-coumaroyl)-2-hydroxy-urs-12-en-28-oic acid (8). The ethanol extract and ethyl acetate
fraction presented total phenolic contents of 10.6 ± 0.1 and 11.4 ± 0.3 mg GAE
g-1, respectively, and considerable antioxidant activity in the DPPH
assay with EC50 values of 299.4 ± 1.5 and 391.8 ± 0.9 μg·ml-1,
respectively.