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has been cited by the following article:

• TITLE: Effect of Citric Acid, Phytase and Calcium Levels on the Calcium and Phosphorus Content in Egg: Yolk-Albumen and Shell, Yolk Color and Egg Quality in Diets of Laying Hens

  AUTHORS: Lily M. Vargas-Rodríguez, Jesús E. Morales-Barrera, José G. Herrera-Haro, Juan Antonio-Bautista, Roberto López-Pozos, David Hernández-Sánchez

  KEYWORDS: Citric Acid, Phytase, Calcium, Laying Hens, Eggshell

  JOURNAL NAME: Food and Nutrition Sciences, Vol.7 No.14, December 9, 2016

  ABSTRACT: To evaluate the effect of different levels of citric acid (CA) combined with phytase and calcium levels in the diets of laying hens on the calcium and phosphorus (P) contents of eggs: yolk-albumen and shell, yolk color and egg quality. A study was conducted with 24-week-old laying hens fed with a diet based on sorghum and soybean meal with 2.7 Mcal of EM/kg, 15% crude protein, 3.25% calcium and 0.25% available P. The levels of CA, phytase and calcium were 0%, 0.6% and 1.2%; 0 and 300 units of phytase (PHYU)/kg; and 3.0% and 3.25%, respectively. Phytase was added as an ingredient into to the diets, contributing 0.1% P and 0.3% calcium. A completely randomized factorial experiment was performed with a 3 × 2 × 2 design and 4 replications. The CA increased (P