TITLE:
The Control of Lactobacillus sp. by Extracellular Compound Produced by Pseudomonas aeruginosa in the Fermentation Process of Fuel Ethanol Industry in Brazil
AUTHORS:
Cíntia Greice Matsuoca Góis, Lucilene Lopes-Santos, Jamile Priscila de Oliveira Beranger, Admilton Gonçalves de Oliveira, Flavia Regina Spago, Galdino Andrade
KEYWORDS:
Fuel Ethanol; Fermentation; Contamination; Lactobacillus; Yeast; Pseudomonas
JOURNAL NAME:
Journal of Sustainable Bioenergy Systems,
Vol.3 No.3,
September
13,
2013
ABSTRACT:
This work evaluated the
effect of secondary bacterial metabolites produced by Pseudomonas sp LV strain in control of Lactobacillus sp. population in the microcosm of the vat during ethanol
fermentation. The fraction F4 produced by Pseudomonas
aeruginosa was extracted with dichloromethane and fractionating by vacuum
liquid chromatography obtained in a methanol phase. The evaluation of
antibiotic activity of F4 fraction mixed or not with sulphuric acid and Kamoram?.
The antibiotic activity of F4 fraction was determined as well as the
fermentation efficiency. Also was determined yeast cell viability, budding
formation, the viability of budding cells, and number of populations of Saccharomyces cerevisiae and Lactobacillus sp. The results showed
that the F4 fraction had high selective antibiotic activity against Lactobacillus sp. but not for S. cerevisae, and no inhibitory effect
was observed in the fermentation process by yeast. Also F4 fraction decreased flocculation
and foam formation. The F4 has an antibiotic activity against Lactobacillus sp. and should be used as an alternative
to control bacteria contamination and foam and flocculation formation in the fuel ethanol fermentation process. The F4 fraction
could reduce the use of antibiotics in the control of Lactobacillus sp. population during the fuel ethanol production.