Share This Article:

Activity of Secondary Bacterial Metabolites in the Control of Citrus Canker

Abstract Full-Text HTML XML Download Download as PDF (Size:423KB) PP. 295-303
DOI: 10.4236/as.2015.63030    4,813 Downloads   5,432 Views   Citations

ABSTRACT

This study investigated the protective effects of secondary bacterial metabolites, produced by Pseudomonas sp. (bacterium strain LN), on citrus canker disease caused by Xanthomonas axonopodis pv. citri (Xac 306). The LN bacteria strain was cultured in liquid medium and the supernatant free-cells was treated with methanol (AMF) and ethyl acetate (AEF), respectively, and then the extract was concentrated, filtrated, lyophilized and fractionated by vacuum liquid chromatography (VLC). After VLC, eight fractions were obtained. All fractions’ activity against Xac 306 by agar well diffusion assay and minimum inhibitory concentration but in different concentrationswere tested. Cytotoxicity effects were observed in all fractions in 50 μg·mL-1 concentration. The comet assay demonstrated that the fractions EAF, VLC2 and VLC3 presented no genotoxic effects at tested concentrations. In plants only VLC3 showed significant results (p<0.05), reducing the incidence of citrus canker lesions.

Conflicts of Interest

The authors declare no conflicts of interest.

Cite this paper

Murate, L. , Oliveira, A. , Higashi, A. , Barazetti, A. , Simionato, A. , Silva, C. , Simões, G. , Santos, I. , Ferreira, M. , Cely, M. , Navarro, M. , Freitas, V. , Nogueira, M. , Mello, J. , Leite Jr., R. and Andrade, G. (2015) Activity of Secondary Bacterial Metabolites in the Control of Citrus Canker. Agricultural Sciences, 6, 295-303. doi: 10.4236/as.2015.63030.

References

[1] Gottwald, T.R., Sun, X., Riley, T., Graham, J.H., Ferrandino, F. and Taylor, E.L. (2002) Geo-Referenced Spatiotemporal Analysis of the Urban Citrus Canker Epidemic in Florida. Phytopathology, 92, 361-377.
http://dx.doi.org/10.1094/PHYTO.2002.92.4.361
[2] Schubert, T.S. and Sun, X. (2003) Bacterial Citrus Canker. Plant Pathology Circular, 377, 1-6.
[3] Schubert, T.S., Rizbi, S.A., Gottwald, T.R., Graham, J.H. and Dixon, W.N. (2001) Meeting the Challenge of Eradicating Citrus Canker in Florida—Again. Plant Disease, 85, 340-356.
http://dx.doi.org/10.1094/PDIS.2001.85.4.340
[4] Rudolph, K.(1993) Infection of the Plant by Xanthomonas. In: Swings, J.G. and Civerolo, E.L., Eds., Xanthomonas,Chapman and Hall, London, 193-264.
http://dx.doi.org/10.1007/978-94-011-1526-1_4
[5] Koizumi, M.(1985) Citrus Canker: The World Situation. In: Timmer, L.W., Ed.,Citrus Canker: An International Perspective, University of Florida, Institute of Food and Agricultural Science,Gainesville, 2-7.
[6] Quimby, P.C., King, L.R. and Grey, W.E. (2002) Biological Control as Means of Enhancing the Sustainability of Crop/Land Management Systems. Agriculture, Ecosystems and Environment, 88, 147-152.
http://dx.doi.org/10.1016/S0167-8809(01)00251-1
[7] Foldes, T., Bánhegyi, I., Herpai, Z., Varga, L. and Szigeti, J. (2000) Isolation of BacillusStrains from Rhizosphere of Cereals and in Vitro Screening for Antagonism against Phytopatogenic, Food-Borne Pathogenic and Spoil-Age Microorganisms. Journal of Applied Microbiology, 89, 840-846.
http://dx.doi.org/10.1046/j.1365-2672.2000.01184.x
[8] de Oliveira, A.G., Murate, L.S., Spago, F.R., Lopes, L.D., Beranger, J.P.D., Martin, J.A.B.S., Nogueira, M.A., de Mello, J.C.P., Andrade, C.G.T.D. and Andrade, G. (2011) Evaluation of the Antibiotic Activity of Extracellular Compounds Produced by the PseudomonasStrain against the Xanthomonas citri pv. citri 306 Strain. Biological Control, 56, 125-131.
http://dx.doi.org/10.1016/j.biocontrol.2010.10.008
[9] Lopes, L.P., Oliveira, A.G., Beranger, J.P.O., Gois, C.G., Vasconcellos, F.C.S., San Martin, J.A.B., Andrade, C.G.T.J., Mello, J.C.P. and Andrade, G. (2012) Activity of Extracellular Compounds of Pseudomonas sp. against Xanthomonas axonopodisin Vitroand Bacterial Leaf Blight in Eucalyptus. Tropical Plant Pathology, 37, 233-238.
http://dx.doi.org/10.1590/S1982-56762012000400001
[10] Vasconcellos, F.C.S., de Oliveira, A.G., Lopes-Santos, L., Beranger, J.P.O., Cely, M.V.T., Simionato, A.S., Pistori, J.F., Spago, F.R., Mello, J.C.P., San Martin, J.A.B., Andrade, C.G.T.J. and Andrade, G. (2014)Evaluation of Antibiotic Activity Produced by Pseudomonas aeruginosa LV Strain against Xanthomonas arboricola pv. pruni.Agricultural Sciences, 5, 71-76.
http://dx.doi.org/10.4236/as.2014.51008
[11] da Silva, A.C.R., Ferro, J.A., Reinach, F.C., Farah, C.S., Furlan, L.R., Quaggio, R.B., Monteiro-Vitorello, C.B., Sluys, M.A.V., Almeida, N.F., Alves, L.M.C., do Amaral, A.M., Bertolini, M.C., Camargo, L.E.A., Camarotte, G., Cannavan, F., Cardozo, J., Chambergo, F., Ciapina, L.P., Cicarelli, R.M.B., Coutinho, L.L., Cursino-Santos, J.R., El-Dorry, H., Faria, J.B., Ferreira, A.J.S., Ferreira, R.C.C., Ferro, M.I.T., Formighieri, E.F., Franco, M.C., Greggio, C.C., Gruber, A., Katsuyama, A.M., Kishi, L.T., Leite, R.P., Lemos, E.G.M., Lemos, M.V.F., Locali, E.C., Machado, M.A., Madeira, A. M.B.N., Martinez-Rossi, N.M., Martins, E.C., Meidanis, J., Menck, C.F.M., Miyaki, C.Y., Moon, D.H., Moreira, L.M., Novo, M.T.M., Okura, V.K., Oliveira, M.C., Oliveira, V.R., Pereira, H.A., Rossi, A., Sena, J.A.D., Silva, C., de Souza, R.F., Spinola, L.A.F., Takita, M.A., Tamura, R.E., Teixeira, E.C., Tezza, R.I.D., Trindade dos Santos, M., Truffi, D., Tsai, S.M., White, F.F., Setubal, J.C. and Kitajima, J.P. (2002)Comparison of the Genomes of Two XanthomonasPathogens with Differing Host Specificities. Nature, 417, 459-463.
http://dx.doi.org/10.1038/417459a
[12] Rampazo, L.G.L. (2007) Evaluation of the Effect of Biological Agents and Their Products into the Incidence of Citrus Canker Lesions. Master Thesis, University of Londrina, Londrina.
[13] Cain, C.C., Henry, A.T., Waldo III, R.H., Casida Jr., L.J. and Falkinham III, J.O. (2000) Identification and Characteristics as a Novel BurkholderiaStrain with Broad-Spectrum Antimicrobial Activity. Applied and Environmental Microbiology, 66, 4139-4141.
http://dx.doi.org/10.1128/AEM.66.9.4139-4141.2000
[14] Mello, J.C.P. and Petrovick, P.R. (2000) Quality Control of Baccharis trimera (Less.) DC. (Astearaceae) Hydroalcoholic Extracts. Acta Farmaceutica Bonaerense, 19, 211-215.
[15] Speit, G. and Hartmann, A. (1999) The Comet Assay (Single-Cell Gel Test), a Sensitive Genotoxicity Test for the Detection of DNA Damage and Repair. In: Henderson, D.S., Ed., Methods in Molecular Biology: DNA Repair Protocols—Eukaryotic Systems, Humana Press, Totowa, 203-211.
[16] Singh, N.P., McCoy, M.T., Tice, R.R. and Schneider, E.L. (1988) A Simple Technique for Quantitation of Low Levels of DNA Damage in Individual Cells. Experimental Cell Research, 175, 184-191.
http://dx.doi.org/10.1016/0014-4827(88)90265-0
[17] Hewitt, E.J. (1966) Sand and Water Culture Methods Used in Study of Plant Nutrition. 2nd Edition England, Commonwealth Agricultural Bureaux, Farnham Royal, Bucks, England.
[18] Cardozo, V.F., de Oliveira, A.G., Nishio, E.K., Perugini, M.R.E., Andrade, C.G.T.J., Silveira, W.D., Durán, N., Andrade, G., Kobayashi, R.K.T. and Nakazato, G. (2013) Antibacterial Activity of Extracellular Compounds Produced by a Pseudomonas Strain against Methicillin-Resistant Staphylococcus aureus (MRSA) Strains. Annals of Clinical Microbiology and Antimicrobials, 12, 1-8.
[19] Góis, C.G.M., Lopes-Santos, L., Beranger, J.P.O., de Oliveira, A.G., Spago, F.R. and Andrade, G. (2013) The Control of Lactobacillus sp. by Extracellular Compound Produced by Pseudomonas aeruginosa in the Fermentation Process of Fuel Ethanol Industry in Brazil. Journal of Sustainable Bioenergy Systems, 3, 194-201.
http://dx.doi.org/10.4236/jsbs.2013.33027
[20] Hartmann, A. and Speit, G. (1997) The Contribution of Cyto-Toxicity to DNA-Effects in the Single Cell Gel Test (Comet Assay). Toxicology Letters, 90, 183-188.
http://dx.doi.org/10.1016/S0378-4274(96)03847-7
[21] Koizumi, M. (1988) Mechanisms of Disease Development and Host Resistance of Citrus Canker. In: Rossetti, V., Ed., Processing of International Symposium of Citrus Canker, Blight and Similar Diseases, Fundacao Cargil, Campinas, 138-145.
[22] Cook, R.J., Bruckart, W.L., Coulson, J.R., Goettel, M.S., Humber, R.A., Lumsdem, R.D., Maddox, J.V., Mcmanus, M.L., Moore, L., Meyer, S.F., Quimby, P.C., StackJr., J.P. and Vaughn, J.L. (1996) Safety of Microorganisms Intended for Pest and Plant Disease Control: A Framework for Scientific Evaluation. Biological Control, 7, 333-351.
http://dx.doi.org/10.1006/bcon.1996.0102

  
comments powered by Disqus

Copyright © 2018 by authors and Scientific Research Publishing Inc.

Creative Commons License

This work and the related PDF file are licensed under a Creative Commons Attribution 4.0 International License.