Qualitative Evaluation of the Capacity of Lactobacillus Strains to Degrade Mycotoxins Developed and Accumulated by Strains of the Genus Alternaria

L. Pérez, G. Girmé, E. L. Arosemena, M. A. Calvo Torras

Grupo de Investigación en Microbiología Aplicada y Medio-Ambiental, Departamento de Sanidad y de Anatomia Animales, Fa- cultad de Veterinaria, Universidad Autónoma de Barcelona, Bellaterra, Spain..

DOI: 10.4236/fns.2013.47A004                

Abstract

Mycotoxins are secondary metabolites produced and accumulated by mould. This study assessed the ability of 89 lactic acid bacteria, isolated from different sources, to degrade two mycotoxins by Alternaria: alternariol and altenuene. The mehodology employed was the thin layer chromatography. The results obtained allow us to demonstrate that a 16.85% of the strains (15/89 strains) are capable of decreasing the intensity of the band related at the alternariol and 14.60% (13/89 strains) is capable of decrease the intensity of the band that manifests the altenuane in Alufolium. All tests have been observed under wavelengths of 360 nm and 255 nm. The results obtained allow carrying out a selection of lactic acid strains capable of degrading or decreasing the concentration of mycotoxins selected for this.

Keywords

Lactic Acid Bacteria; Mycotoxins; Alternaria; Thin Layer Chromatography

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1]	C. F. Mayer, “Endemic Panmyelotoxicoses in the Russian Grain Belt. Part One: The Clinical Aspects of Alimentary Toxic Aleukia (ATA). A Comprehensive Review,” Military Sergeon, Vol. 113, No. 3, 1953, pp. 173-189.
[2]	R. D. Coker, “Mycotoxins and Their Control: Constraints and Opportunities,” NRI Bulletin 73, Natural Resources Institute, Chatham, 1997.
[3]	J. D. Miller, “Conference Report: 6th International Working Conference on Stored Product Protection,” Australian Mycotoxin Newsletter, Vol. 5, No. 2, 1994, pp. 1-8.
[4]	E. Smith, G. L. Solomons, C. W. Lewis and J. G. Anderson, “Mycotoxins in Human Nutrition and Health,” Directorate-General XII Science, Research and Development, European Commision, 1994.
[5]	K. H. Schleifer and E. Stackenbrandt, “Molecular Systematic of Prokaryotes,” Annual Review of Microbiology, Vol. 37, 1983, pp. 143-187. doi:10.1146/annurev.mi.37.100183.001043
[6]	W. P. Hammes and R. F. Vogel, “The Genus Lactobacillus,” In: The Genera of Lactic Acid Bacteria, Chapman & Hall, London, 1995, pp. 19-54. doi:10.1007/978-1-4615-5817-0_3
[7]	E. Ringo and F. Gatesoupe, “Lactic Acid Bacteria in Fish: A Review,” Aquaculture, Vol. 160, No. 3-4, 1998, pp. 177-179. doi:10.1016/S0044-8486(97)00299-8
[8]	A. Fábrega, “Estudio de la Variabilidad Morfológica y Producción de Metabolitos Secundarios en Cepas del Género Alternaria,” Ph.D. Dissertation, Universidad de Barcelona, Spain, 2001.
[9]	G. E. Templeton, “Alternaria Toxins Related to Pathogenesis in Plants,” In S. Kadis, A. Ciegler and S. J. Ajl, Eds., Microbial Toxins, Vol. VII, Academic Press, Inc., New York, 1972.
[10]	M. Kostecki, S. Crabarkiewicz and J. Szczesna, “Biosynthesis and Preparation of Five Alternaria Metabolites,” Mycotoxin Research, Vol. 7, No. 1, 1991, pp. 3-7.
[11]	R. W. Pero, H. Posner, M. Blois, D. Harvan and J. W. Spalding, “Toxicity of Metabolites Produced by the Alternaria,” Environmental Health Perspectives, Vol. 4, 1973, pp. 87-94.
[12]	M. Rodríguez, “Aislamiento y Selección de Cepas del Género Lactobacillus con Capacidad Probiótica e Inmunomoduladora,” Ph.D. Dissertation, Universidad Autónoma de Barcelona, Spain, 2009.