Stir-Fry Chicken with Green Curry Suppresses Inflammatory Gene Expression by Lipopolysaccharide-Induced Murine Macrophages

.
DOI: 10.4236/fns.2011.27106   PDF   HTML   XML   3,462 Downloads   7,022 Views   Citations

Abstract

Inflammatory mediators produced during inflammatory response play an important role on pathological development of several chronic diseases. Although several dietary plants exhibited anti-inflammatory property, their impacts as a whole food has been rarely reported. The aim of the present study is to assess anti-inflammatory activity of an ethanol extract from a whole food namely “ready to eat stir-fry chicken with green curry” consisting of green curry paste, big egg plant, pea egg plant, red chili, kaffer lime and sweet basil as plant-based ingredients. The food extract at 55 - 220 μg/ml was incubated with RAW264.7 murine macrophage cells prior to stimulation with lipopolysaccharide (LPS) for 24 h. Inflammatory mediators [(inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), tumor necrosis factor (TNF-α) and interleukine-6 (IL-6)] mRNA and protein were determined by RT-PCR, immunoblot and ELISA respectively. The modulation mechanism by the food extract was observed by measuring the phosphorylated form of mitogen activated protein kinases (MAPKs) and inhibitor kappa B alpha (IκB-α). The ready to eat stir-fry chicken with green curry extract significantly suppressed LPS-induced iNOS, COX-2, IL-6 and TNF-α gene expression in a dose-depend- ent manner without cytotoxicity. The suppressive effect was modulated partly by inhibiting phosphorylation of MAPKs and IκB-α. These results indicate that spices and vegetables in a complex diet still possess strong anti-inflammatory activities which warrant confirming such activities to ameliorate the pathogenesis of inflammatory-associated chronic diseases in vivo.

Share and Cite:

S. Charoenkiatkul, C. Muangnoi, P. Chingsuwanrote, P. Praengamthanachoti, S. Tuntipopipat and S. Svasti, "Stir-Fry Chicken with Green Curry Suppresses Inflammatory Gene Expression by Lipopolysaccharide-Induced Murine Macrophages," Food and Nutrition Sciences, Vol. 2 No. 7, 2011, pp. 770-779. doi: 10.4236/fns.2011.27106.

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1] B. B. Aggarwal, “Nuclear Factor-[Kappa] B: The Enemy within,” Cancer Cell, Vol. 6, No. 3, 2004, pp. 203-208. doi:10.1016/j.ccr.2004.09.003
[2] M. Guha and N. Mackman, “LPS Induction of Gene Expression in Human Monocytes,” Cellular Signalling, Vol. 13, No. 2, 2001, pp. 85-94. doi: 10.1016/S0898-6568(00)00149-2
[3] S. I. Grivennikov and M. Karin, “Inflammatory Cytokines in Cancer: Tumour Necrosis Factor and Interleukin 6 Take the Stage,” Annals of the Rheumatic Diseases, Vol. 70, 2011, pp.104-108. doi:10.1136/ard.2010.140145
[4] A. Scalbert, C. Andres-Lacueva, M. Arita, P. Kroon, C. Manach and M. Urpi-Sarda, “Databases on food phytochemicals and their health promoting effects,” Journal of Agricultural and Food Chemistry, Vol. 59, No. 9, 2011, pp. 4331-4348. doi:10.1021/jf200591d
[5] B. B. Aggarwal, M. E. Van Kuiken, L. H. Iyer, K. B. Harikumar and B. Sung, “Molecular Targets of Nutraceuticals Derived from Dietary Spices: Potential Role in Suppression of Inflammation and Tumorigenesis,” Experimental Biology and Medicine, Vol. 234, No. 8, 2009, pp. 825-849. doi:10.1158/1078-0432.CCR-08-0149
[6] H. Wu-Yang, C. Yi-Zhong and Z. Yanbo, “Natural Phenolic Compounds from Medicinal Herbs and Dietary Plants: Potential Use for Cancer Prevention,” Nutrition & Cancer, Vol. 62, No. 1, 2011, pp. 1-20. doi:10.1080/01635580903191585
[7] H. M. Merken and G. R. Beecher, “Liquid Chroma-Tographic Method for the Separation and Quantification of Prominent Flavonoid Aglycones,” Journal of Chromatography A, Vol. 897, No.1-2, 2000, pp.177-184. doi:10.1016/S0021-9673(00)00826-8
[8] V. Vichai and K. Kirtikara, “Sulforhodamine B Colorimetric Assay for Cytotoxicity Screening,” Nature Protocol, Vol. 1, No. 3, 2006, pp. 1112-1116. doi: 10.1038/nprot.2006.179
[9] L. C. Green, D. A. Wagner, J. Glogowski, P. L. Skipper, J. S. Wishnok and S. R. Tannenbaum, “Analysis of Nitrate, Nitrite, and [15N] Nitrate in Biological Fluids,” Analytical Biochemistry, Vol. 126, 1982, pp. 131-138. doi:10.1016/0003-2697(82)90118-X
[10] S. Tuntipopipat, C. Muangnoi, P. Chingsuwanrote, M. Parengam, P. Chantravisut and S. Charoenkiatkul, “Anti-Inflammatory Activities of Red Curry Paste Extract on Lipopolysaccharide-Activated Murine Macrophage Cell Line,” Nutrition, Vol. 27, No. 4, 2011, pp. 479-487. doi:10.1016/j.nut.2010.04.009
[11] L. F. de Souza, F. Barreto, E. G. da Silva, M. E. Andrades, E. L. M. Guimares and G. A. Behr, “Regulation of LPS Stimulated ROS Production in Peritoneal Macrophages from Alloxan-Induced Diabetic Rats: Involvement of High Glucose and PPAR [Gamma],” Life Science, Vol. 81, 2007, pp. 153-159. doi:10.1016/j.lfs.2007.04.035
[12] N. Nakatani, S.-I. Kayano, H. Kikuzaki, K. Sumino, K. Katagiri and T. Mitani, “Identification, Quantitative de-Termination, and Antioxidative Activities of Chlorogenic Acid Isomers in Prune (Prunus domestica L.),” Journal of Agricultural and Food Chemistry, Vol. 48, No. 11, 2000, pp. 5512-5516. doi:10.1021/jf000422s
[13] U. Boettler, N. Volz, G. Pahlke, N. Teller, C. Kotyczka and V. Somoza, “Coffees Rich in Chlorogenic Acid or N-Methylpyridinium Induce Chemopreventive Phase II-En-zymes via the Nrf2/ARE Pathway in Vitro and in Vivo,” Molecular Nutrition & Food Research, Vol. 55, No. 5, 2011, pp. 798-802. doi:10.1002/mnfr.201100115
[14] C. Hoelzl, S. Knasmüller, K.-H. Wagner, L. Elbling, W. Huber and N. Kager, “Instant Coffee with High Chloro-genic Acid Levels Protects Humans against Oxidative Damage of Macromolecules,” Molecular Nutrition & Food Research, Vol. 54, No. 12, 2010, pp. 1722-1733. doi:10.1002/mnfr.201000048
[15] W.-K. Jung, I. Choi, D.-Y. Lee, S.-S. Yea, Y.-H. Choi and M.-M. Kim, “Caffeic Acid Phenethyl Ester Protects Mice from Lethal Endotoxin Shock and Inhibits Lipopoly-Saccharide-Induced Cyclooxygenase-2 and Inducible Nitric Oxide Synthase Expression in RAW 264.7 Macrophages via the p38/ERK and NF-[kappa]B Pathways,” The International Journal of Biochemistry & Cell Biology, Vol. 40, No. 1, 2008, pp. 2572-2582. doi:10.1016/j.biocel.2008.05.005
[16] M. Nardini, P. Pisu, V. Gentili, F. Natella, M. Di and F. Piccolella, “Effect of Caffeic Acid on Tert-Butyl Hydroper-Oxide-Induced Oxidative Stress in U937,” Free Radical Biology and Medicine, Vol. 25, No. 9, 1998, pp. 1098-1105. doi:10.1016/S0891-5849(98)00180-4
[17] S. Tuntipopipat, C. Muangnoi and M. L. Failla. “Anti-Inflammatory Activities of Extracts of Thai Spices and Herbs with Lipopolysaccharide-Activated RAW 264.7 Murine Macrophages,” Journal of Medicinal Food, Vol. 12, No.6, 2009, pp. 1213-1220. doi:10.1089/jmf.2009.1118
[18] M. Mohan, S. Kamble, P. Gadhi and S. Kasture, “Protective Effect of Solanum Torvum on Doxorubicin-Induced Nephrotoxicity in Rats,” Food and Chemical Toxicology, Vol.48, No.1, 2010, pp. 436-440. doi:10.1016/j.fct.2009.10.042
[19] S.-Y. Cho, S.-J. Park, M.-J. Kwon, T.-S. Jeong, S.-H. Bok and W.-Y. Choi, “Quercetin Suppresses Proinflammatory Cytokines Production through MAP Kinases and NF-?B Pathway in Lipopolysaccharide-Stimulated Macrophage,” Molecular and Cellular Biochemistry, Vol. 243, No. 1, 2003, pp. 153-160. doi:10.1023/A:1021624520740
[20] M. Zhang, S. G. Swarts, L. Yin, C. Liu, Y. Tian, Y. Cao, et al., “Antioxidant Properties of Quercetin,” Advances in Experimental Medicine and Biology, Vol 701, Part 10, 2011, pp. 283-289. doi: 10.1007/978-1-4419-7756-4
[21] S. K. Bai, S. J. Lee, H. J. Na, K. S. Ha, J. A. Han, H. Lee, Y. G. Kwon, C. K. Chung and Y. M. Kim, “Beta-Carotene Inhibits Inflammatory Gene Expression in Lipopolysaccha-Ride-Stimulated Macrophages by Suppressing Redox-Based NF-kB Activation,” Experimental and Molecular Medicine, Vol. 37, No. 4, 2005, pp. 323-334
[22] J.-H. Kim, H.-J. Na, C.-K. Kim, J.-Y. Kim, K.-S. Ha and H. Lee, “The Non-Provitamin A Carotenoid, Lutein, Inhib-Itsnf-[Kappa] B-Dependent Gene Expression through Redox-Based Regulation of the Phosphatidylinositol 3-Kinase/PTEN/Akt and NF-[Kappa]B-Inducing Kinase Pathways: Role of H2O2 in NF-[Kappa]B Activation,” Free Radical Biology and Medicine, Vol. 45, No. 6, 2008, pp. 885-896. doi:10.1016/j.freeradbiomed.2008.06.019
[23] A. Xagorari, A. Papapetropoulos, A. Mauromatis, M. Economou, T. Fotsis and C. Roussos, “Luteolin Inhibits an Endotoxin-Stimulated Phosphorylation Cascade and Proinflammatory Cytokine Production in Macrophages,” Journal of Pharmacology and Experimental Therapeutics, Vol. 296, No. 1, 2001, pp. 181-187.
[24] G. K. Harris, Y. Qian, S. S. Leonard, D. C. Sbarra and X. Shi, “Luteolin and Chrysin Differentially Inhibit Cycloo- xy-Genase-2 Expression and Scavenge Reactive Oxygen Species but Similarly Inhibit Prostaglandin-E2 Formation in RAW 264.7 Cells,” The Journal of Nutrition, Vol. 136, No. 6, 2006, pp. 1517-1521.
[25] Y.-C. Liang, Y.-T. Huang, S.-H. Tsai, D. Tsai, S.-Y. Lin-Shiau, C.-F. Chen and J.-K. Lin, “Suppression of inDucible Cyclooxygenase and Inducible Nitric Oxide Synthase by Apigenin and Related Flavonoids in Mouse Macrophages,” Carcinogenesis, Vol. 20, No. 10, 1999, pp. 1945-1952. doi: 10.1093/carcin/20.10.1945

  
comments powered by Disqus

Copyright © 2020 by authors and Scientific Research Publishing Inc.

Creative Commons License

This work and the related PDF file are licensed under a Creative Commons Attribution 4.0 International License.