Effect of Sunlight Exposure and Different Withering Durations on Theanine Levels in Tea (Camellia sinensis)

DOI: 10.4236/fns.2015.611105   PDF   HTML   XML   3,132 Downloads   4,119 Views   Citations

Abstract

Theanine is a non-protein amino acid representing as much as 50% of the total amino acids in black tea and 1% - 2% of dry weight in green tea. It has been shown to be able to reduce high blood pressure, promote relaxation, and inhibit caffeine’s side effects among others. This current study explored the effects of sunlight and withering durations on theanine levels in tea shoots. Theanine content from three leaves and a bud, two leaves and a bud and internodes were detected and quantified by using High Performance Liquid chromatography (HPLC). Sunlight exposure experiment was started at dawn (0600 HRS, GMT + 3.00) when the light intensity was low and tea was collected at three-hour interval throughout the day to (1800 Hrs, GMT + 3.00) when the light intensity had dropped. At the start of the experiment, the theanine levels were significantly high but as the intensity of sunlight increased during the day there was a significant drop in theanine levels, and as the sun set the theanine levels increased significantly again in all samples. The results also showed that theanine levels were significantly increased after 15 hours of withering. Three leaves and a bud withered for 3 hours had mean theanine levels of 1.41% and those withered for 15 hours had mean theanine levels of 3.11%. Internodes exhibited higher mean theanine levels than those of leaves. In the light of these results, it’s evident that withering for a longer period of time and harvesting of tea when the light intensity is low ensure high amount of theanine in tea.

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Too, J. , Kinyanjui, T. , Wanyoko, J. and Wachira, F. (2015) Effect of Sunlight Exposure and Different Withering Durations on Theanine Levels in Tea (Camellia sinensis). Food and Nutrition Sciences, 6, 1014-1021. doi: 10.4236/fns.2015.611105.

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1] Alan, C.A., Jaganath, I.B. and Clifford, M.N. (2009) Dietary Phenolics: Chemistry, Bioavailability and Effects on Health. Natural Product Reports, 26, 1001-1043.
http://dx.doi.org/10.1039/b802662a
[2] Dufresne, C.J. and Farnworth, E.R. (2001) A Review of Latest Research Findings on the Health Promotion Properties of Tea. The Journal of Nutritional Biochemistry, 12, 404-421.
http://dx.doi.org/10.1016/S0955-2863(01)00155-3
[3] Kuo, K.L., Weng, M.S., Chiang, C.T., Tsai, Y.J., Lin-Shiau, S.Y. and Lin, J.K. (2005) Comparative Studies on the Hypolipidemic and Growth Suppressive Effects of Oolong, Black, Pu-Erh, and Green Tea Leaves in Rats. Journal of Agricultural and Food Chemistry, 53, 480-489.
http://dx.doi.org/10.1021/jf049375k
[4] Cheng, I.F. and Breen, K. (2000) On the Ability of Four Flavonoids, Baicilein, Luteolin, Naringenin, and Quercetin, to Suppress the Fenton Reaction of the Iron-ATP Complex. Biometals, 13, 77-83.
http://dx.doi.org/10.1023/A:1009229429250
[5] Zhao, J.W., Chen, Q.S. and Huang, X.Y. (2006) Qualitative Identification of Tea Categories by near Infrared Spectroscopy and Support Vector Machine. Journal of Pharmaceutical and Biomedical Analysis, 41, 1198-1204.
http://dx.doi.org/10.1016/j.jpba.2006.02.053
[6] Khan, N. and Mukhtar, H. (2007) Tea Polyphenols for Health Promotion. Life Science, 81, 519-533.
http://dx.doi.org/10.1016/j.lfs.2007.06.011
[7] Yang, C.S., Maliakal, P. and Meng, X.F. (2002) Inhibition of Carcinogenesis by Tea. Annual Review of Pharmacology and Toxicology, 42, 25-54.
http://dx.doi.org/10.1146/annurev.pharmtox.42.082101.154309
[8] Fujimura, Y., Tachibana, H. and Yamada, K. (2004) Lipid Raft-Associated Catechin Suppresses the FcεRI Expression by Inhibiting Phosphorylation of the Extracellular Signal-Regulated Kinase1/2. FEBS Letters, 556, 204-210.
http://dx.doi.org/10.1016/S0014-5793(03)01432-7
[9] Bolling, B.W., Chen, C.Y. and Blumberg, J.B. (2009) Tea and Health: Preventive and Therapeutic Usefulness in the Elderly. Current Opinion in Clinical Nutrition & Metabolic Care, 12, 42-48.
http://dx.doi.org/10.1097/MCO.0b013e32831b9c48
[10] Gradisar, H., Pristovsek, P., Plaper, A. and Jerala, R. (2007) Green Tea Catechins Inhibit Bacterial DNA Gyrase by Interaction with Its ATP Binding Site. Journal of Medicinal Chemistry, 50, 264-271.
http://dx.doi.org/10.1021/jm060817o
[11] Morley, N., Clifford, T., Salter, L., Campbell, S., Gould, D. and Curnow, A. (2005) The Green Tea Polyphenol (-)-Epigallocatechingallate and Green Tea Can Protect Human Cellular DNA from Ultraviolet and Visible Radiation-Induced Damage. Photodermatology, Photoimmunology and Photomedicine, 21, 15-22.
http://dx.doi.org/10.1111/j.1600-0781.2005.00119.x
[12] Kito, M., Kokura, H., Izaki, J. and Sasaoka, K. (1968) Theanine, a Precursor of the Phloroglucinol Nucleus of Catechins in Tea Plants. Phytochemistry, 7, 599-603.
http://dx.doi.org/10.1016/S0031-9422(00)88234-5
[13] Sakato, Y. (1949) The Chemical Constituents of Tea: A New Amide Theanine. Journal of Agricultural and Food Chemistry, 23, 262-267.
[14] Syu, K.Y., Lin, C.L., Huang, H.C. and Lin, J.K. (2008) Determination of Theanine, GABA, and Other Amino Acids in Green, Oolong, Black, and Pu-Erh Teas with Dabsylation, and High Performance Liquid Chromatography. Journal of Agricultural and Food Chemistry, 56, 7637-7643.
http://dx.doi.org/10.1021/jf801795m
[15] Deng, W.W., Ogita, S. and Ashihara, H. (2010) Distribution and Biosynthesis of Theanine in Theaceae Plants. Plant Physiology and Biochemistry, 47, 70-72.
[16] Hara, Y., Luo, S.J., Wikramasinghe, R.L. and Yamanishi, T. (1995) Special Issue on Tea. Food Reviews International, 11, 371-545.
[17] Palva, S. and Palva, J.M. (2007) New Vistas for α-Frequency Band Oscillations. Trends in Neurosciences, 30, 150-158.
http://dx.doi.org/10.1016/j.tins.2007.02.001
[18] Kaneko, S., Kumazawa, K. and Masuda, H. (2006) Molecular and Sensory Studies on the Umami Taste of Japanese Green Tea. Journal of Agricultural and Food Chemistry, 54, 2688-2694.
http://dx.doi.org/10.1021/jf0525232
[19] Le Gall, G., Colquhoun, I.J. and Defernez, M. (2004) Metabolite Profiling Using Proton NMR Spectroscopy for Quality Assessment of Green Tea, Camellia sinensis (L.). Journal of Agricultural and Food Chemistry, 52, 692-700.
http://dx.doi.org/10.1021/jf034828r
[20] Thippeswamy, R., Mallikarjun, K.G., Gouda, M., Rao, D.H., Martin, A. and Gowda, L.R. (2006) Determination of Theanine in Commercial Tea by Liquid Chromatography with Fluorescence and Diode Array Ultraviolet Detection. Journal of Agricultural and Food Chemistry, 54, 7014-7019.
http://dx.doi.org/10.1021/jf061715+
[21] Lee, J.E., Lee, B.J., Chung, J.O., Hwang, J.A., Lee, S.J. and Lee, C.H. (2010) Geographical and Climatic Dependencies of Green Tea (Camellia sinensis) Metabolites: A 1H NMR-Based Metabolomics Study. Journal of Agricultural and Food Chemistry, 58, 10582-10589.
http://dx.doi.org/10.1021/jf102415m
[22] Kamath, A.B., Wang, L., Das, H., Li, L., Reinhold, V.N. and Bukowski, J.F. (2003) Antigens in Tea-Beverage Prime Human Vγ2Vδ2 T Cells in Vitro and in Vivo for Memory and Nonmemory Antibacterial Cytokine Responses. Proceedings of the National Academy of Science of the United States of America, 100, 6009-6014.
http://dx.doi.org/10.1073/pnas.1035603100
[23] Kimura, K., Ozeki, M., Juneja, L.R. and Ohira, H. (2007) L-Theanine Reduces Psychological and Physiological Stress Responses. Biological Psychology, 74, 39-45.
http://dx.doi.org/10.1016/j.biopsycho.2006.06.006
[24] Sugiyama, T. and Sadzuka, Y. (2003) Theanine and Glutamate Transporter Inhibitors Enhance the Antitumor Efficacy of Chemotherapeutic Agents. Biochimica et Biophysica Acta, 5, 47-59.
http://dx.doi.org/10.1016/s0304-419x(03)00031-3
[25] Yamada, T. and Terashima, T. (2009) Theanine, Gamma-Glutamylethylamide, a Unique Amino Acid in Tea Leaves, Modulates Neurotransmitter Concentrations in the Brain Striatum Interstitium in Conscious Rats. Amino Acids, 36, 21- 27.
http://dx.doi.org/10.1007/s00726-007-0020-7
[26] Zhang, L., Zhang, Z.Z., Lu, Y.N., Zhang, J.S. and Preedy, V.R. (2013) L-Theanine from Green Tea: Transport and Effects on Health. In: Preedy, V.R., Ed., Tea in Health and Disease Prevention, Chap. 35, Academic Press, Waltham, 425-435.
http://dx.doi.org/10.1016/B978-0-12-384937-3.00035-5
[27] Cho, H.S., Kim, S., Lee, S.Y., Park, J.A., Kim, S.J. and Chun, H.S. (2008) Protective Effect of the Green Tea Component, L-Theanine on Environmental Toxins-Induced Neuronal Cell Death. Neuro Toxicology, 26, 656-662.
http://dx.doi.org/10.1016/j.neuro.2008.03.004
[28] Egashira, N., Hayakawa, K., Mishima, K., Kimura, H., Iwasaki, K. and Fujiwara, M. (2004) Neuroprotective Effect of γ-Glutamylethylamide (Theanine) on Cerebral Infraction in Mice. Neuroscience Letters, 36, 58-61.
http://dx.doi.org/10.1016/j.neulet.2004.03.046
[29] Zheng, G., Bamba, K., Okubo, T., Juneja, L.R. and Sayama, I.K. (2005) Effect of Theanine, γ-Glutamylethylamide, on Bodyweight and Fat Accumulation in Mice. Animal Science Journal, 76, 153-157.
http://dx.doi.org/10.1111/j.1740-0929.2005.00251.x
[30] Kilel, E.C., Faraj, A.K., Wanyoko, J.K., Wachira, F.N. and Mwingirwa, V. (2013) Green Tea from Purple Leaf Coloured Tea Clones in Kenya—Their Quality Characteristics. Journal of Food Chemistry, 141, 769-775.
http://dx.doi.org/10.1016/j.foodchem.2013.03.051
[31] Chu, D.C., Kobayashi, K., Juneja, L.R. and Yamamoto, T. (1997) Theanine—Its Synthesis, Isolation and Physiological Activity. In: Yamamoto, T., Ed., Chemistry and Applications of Green Tea, CRC Press, Boca Raton, 129-135.
[32] Kyle, J.A.M., Morrice, P.C., McNeill, G. and Duthie, G.G. (2007) Effects of Infusion Time and Addition of Milk on Content and Absorption of Polyphenols in Black Tea. Journal of Agricultural and Food Chemistry, 55, 4889-4894.
http://dx.doi.org/10.1021/jf070351y
[33] Chen, C.N., Liang, C.M., Lai, J.R., Tsai, Y.J., Tsay, J.S. and Lin, J.K. (2003) Capillary Electrophoretic Determination of Theanine, Caffeine, and Catechins in Fresh Tea Leaves and Oolong Tea and Their Effects on Rat Neurosphere Adhesion and Migration. Journal of Agricultural and Food Chemistry, 51, 7495-7503.
http://dx.doi.org/10.1021/jf034634b
[34] Baptista, J., Lima, E., Paiva, L., Andrade, L.A. and Alves, M.G. (2012) Comparison of Azorean Tea Theanine to Teas from Other Origins by HPLC/DAD/FD. Effect of Fermentation, Drying Temperature, Drying Time and Shoot Maturity. Journal of Food Chemistry, 132, 2181-2187.
http://dx.doi.org/10.1016/j.foodchem.2011.12.050
[35] Chen, Y.L., Jiang, Y.M., Duan, J., Shi, J., Xue, S. and Kakuda, Y. (2010) Variation in Catechin Contents in Relation to Quality of “Huang Zhi Xiang” Oolong Tea (Camellia sinensis) at Various Growing Altitudes and Seasons. Food Chemistry, 119, 648-652.
http://dx.doi.org/10.1016/j.foodchem.2009.07.014
[36] Song, R., Kelman, D., Johns, K.L. and Wright, A.D. (2012) Correlation between Leaf Age, Shade Levels and Characteristic Beneficial Natural Constituents of Tea (Camellia sinensis) Grown in Hawaii. Journal of Food Chemistry, 133, 707-714.
http://dx.doi.org/10.1016/j.foodchem.2012.01.078
[37] Jabeen, S., Alam, S., Saleem, M., Ahmad, W., Bibi, R., Hamid, F.S. and Shah, H.U. (2015) Withering Timings Affect the Total Free Amino Acids and Mineral Contents of Tea Leaves during Black Tea Manufacturing. Arabian Journal of Chemistry, 30, 1-7.
http://dx.doi.org/10.1016/j.arabjc.2015.03.011

  
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