Antimicrobial Resistance and Plasmid Profiles of Campylobacter Species from Infants Presenting with Diarrhoea in Osun State, Nigeria

Olutoyin Catherine Adekunle¹, Abiodun A. Onilude²

¹Department of Medical Microbiology and Parasitology, Ladoke Akintola University of Technology, Ogbomoso, Nigeria.
²Department of Microbiology, University of Ibadan, Ibadan, Nigeria.

DOI: 10.4236/ojmm.2015.51003                

Abstract

Antibiotic resistance among enteric bacterial pathogens complicates the heavy diarrhoea disease burden. Antimicrobial resistance of Campylobacter spp. to fluoroquinolones, which are generally used for the treatment of bacterial gastroenteritis, has increased during the past two decades, mainly as a result of the approval of this group of antimicrobials for use in food-producing animals. The aim is to determine the frequency of resistance of campylobacter to various antimicrobial agents and the relationship between antimicrobial agents of the isolates and the presence of plasmid. Twenty five Campylobacter isolates gotten from humans were subjected to antibiotics testing using Kirby Bauer disc diffusion method as well as standard E-test method. The plasmid profile of the isolates was determined using the Alkaline phosphatise procedure. The antimicrobial susceptibility testing of these isolates showed that all were sensitive to Erythromycin and Ciprofloxacin while none was sensitive to co-trimoxazole. The standard organisms were sensitive to co-trimoxazole (80%) and ciprofloxacin (65%) but were resistant to erythromycin (70%). No plasmid was found in streptomycin and ampicillin resistant strains, with the exception of four isolates which were co-trimoxazole-resistant and which contained around 24.4kb plasmids.

Keywords

Campylobacter, Antibiotics, Plasmids

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1]	Kosek, M., Bern, C. and Guerrant, R.L. (2003) The Global Burden of Diarrheal Disease, as Estimated from Studies Published between 1992 and 2000. Bulletin of the World Health Organization, 81, 197-207.
[2]	Coker, A.O. and Adefeso, A.O. (1994) The Changing Patterns of Campylobacter jejuni/coli in Lagos, Nigeria after Ten Years. East African Medical Journal, 71, 437-440.
[3]	Murphy Jr., G.S., Echeverria, P., Jackson, L.R., Arness, MK., LeBron, C. and Pitarangsi, C. (1996) Ciprofloxacin- and Azithromycin-Resistant Campylobacter Causing Traveler’s Diarrhea in US Troops Deployed to Thailand in 1994. Clinical Infectious Diseases, 22, 868-869. http://dx.doi.org/10.1093/clinids/22.5.868
[4]	Hoge, C.W., Gambel, J.M., Srijan, A., Pitarangsi, C. and Echeverria, P. (1998) Trends in Antibiotic Resistance among Diarrheal Pathogens Isolated in Thailand over 15 Years. Clinical Infectious Diseases, 26, 341-345. http://dx.doi.org/10.1086/516303
[5]	Helms, M., Simonsen, J., Olsen, K.E. and Molbak, K. (2005) Adverse Health Events Associated with Antimicrobial Drug Resistance in Campylobacter Species: A Registry-Based Cohort Study. Journal of Infectious Diseases, 191, 1050-1055. http://dx.doi.org/10.1086/428453
[6]	Samuel, M.C., Vugia, D.J., Shallow, S., Marcus, R., Segler, S., McGivern, T., Kassenborg, H., Reilly, K., Kennedy, M., Angulo, F. and Tauxe, R.V., Emerging Infectious Program Food Net Working Group (2004) Epidemiology of Sporadic Campylobacter Infection in the United States and Declining Trend in Incidence. Food Net. 1996-1999. Clinical Infectious Diseases, 38, S165-S174. http://dx.doi.org/10.1086/381583
[7]	Caprioli, A., Busani, L., Martel, J.L. andHelmuth, R. (2000) Monitoring of Antibiotic Resistance in Bacteria of Animal Origin: Epidemiological and Microbiological Methodologies. International Journal of Antimicrobial Agents, 14, 295-301. http://dx.doi.org/10.1016/S0924-8579(00)00140-0
[8]	Stern, N.J., Patton, C.M., Doyle, M.P., Park, C.E. and McCardell, B. (1992) Campylobacter. In: Vanderzant, C. and Splittstoesser, D.F., Eds., Compendium of Methods for the Microbiological Examination of Foods, American Public Health Association, Washington DC, 475-489.
[9]	Engberg, J, Aarestrup, F.M., Taylor, D.E., Gerner-Smidt, P. and Nachamkin, I. (2001) Quinolone and Macrolide Resistance in Campylobacter jejuni and C. coli: Resistance and Trends in Human Isolates. Emerging Infectious Diseases Journal, 7, 24-34. http://dx.doi.org/10.3201/eid0701.010104
[10]	Feierl, G., Wagner, U., Sixl, B., Grisold, A., Daghofer, E. and Marth, E. (2001) Epidemiology of campylobacteriosis and Development of Resistance in Styria. Austria. International Journal of Medical Microbiology, 291, 9.
[11]	Cabrita, J., Rodrigues, J., Braganc Ëa, F., Morgado, C., Pires, I. and Gonc Ëalves, A.P. (1992) Prevalence of Biotypes, Plasmid Profile and Antimicrobial Resistance of Campylobacter Isolated from Wild and Domestic Animals from Northeast Portugal. Journal of Applied Bacteriology, 73, 279-285. http://dx.doi.org/10.1111/j.1365-2672.1992.tb04978.x
[12]	Coker, A.O. and Adefeso, A.O. (1994) The Changing Patterns of Campylobacter jejuni/coli in Lagos, Nigeria after Ten Years. East African Journal Medicine, 71, 437-440.
[13]	Sein, P.P., Hoosen, A.A., Crew-Brown, H.H., Coovadia, Y., Dove, M.G., Heidi, O., Koornhof, H.J., Oliver, S., Perovic, O., Prinsloo, B., Janse van Rensburg, M.N., Simpson, J., Sturm, A.N., Wadula, J. and Wasserman, E. (2005) Antimicrobial Susceptibility Profile of Selected Invasive Pathogens from Academic Hospitals of South Africa for the Ten Years 2001-2004. Southern African Journal of Infectious Diseases, 20, 85-89.