Identification of Sugar, Amino Acids and Minerals from the Pollen of Jandaíra Stingless Bees (Melipona subnitida)

DOI: 10.4236/fns.2014.511112   PDF   HTML   XML   5,484 Downloads   6,980 Views   Citations

Abstract

The aim of this investigation was to analyze two samples of pollen from jandaíra stingless bees (Melipona subnitida) in view of their mineral composition, free amino acids and sugars. Palynological analysis showed that the predominant pollen was monofloral from Senna sp. species (94.5%, pollen 2011) and the second pollen sample showed the presence of two primary species, Chamaecrista sp. (39.2% pollen 2009) and Mimosa tenuiflora (43.5%) (pollen 2009). The highest mineral content was potassium. The bee pollen contained 20.8% and 31.0% of mannitol in samples from 2011 and 2009, respectively. Proline and serine are the predominant amino acids. High content of essential amino acids, minerals and the sugar mannitol confirmed high nutritional value of pollen samples from jandaíra bees.

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da Silva, G. , da Natividade, T. , Camara, C. , da Silva, E. , dos Santos, F. and Silva, T. (2014) Identification of Sugar, Amino Acids and Minerals from the Pollen of Jandaíra Stingless Bees (Melipona subnitida). Food and Nutrition Sciences, 5, 1015-1021. doi: 10.4236/fns.2014.511112.

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1] Bogdanov, S. (2014) Pollen: Nutrition, Functional Properties, Health: A Review. Bee Product Science. http://www.bee-hexagon.net/files/file/fileE/Health/PollenBook2Review.pdf
[2] Silva, T.M.S., Camara, C.A., Lins, A.C.S., Barbosa-Filho, J.M., Silva, E.M.S., Freitas, B.M. and Santos, F.A.R. (2006) Chemical Composition and Free Radical Scavenging Activity of Pollen Loads from Stingless Bee Melipona subnitida Ducke. Journal of Food Composition and Analysis, 19, 507-511. http://dx.doi.org/10.1016/j.jfca.2005.12.011
[3] Silva, T.M.S., Camara, C.A., Lins, A.C.S., Agra, M.F., Silva, E.M.S., Reis, I.T. and Freitas, B.M. (2009) Chemical Composition, Botanical Evaluation and Screening of Radical Scavenging Activity of Collected Pollen by the Stingless Bees Melipona rufiventris (Urucu-Amarela). Anais da Academia Brasileira de Ciências, 81, 173-178. http://dx.doi.org/10.1590/S0001-37652009000200003
[4] Freire, K.R.L., Lins, A.C.S., Dórea, M.C., Santos, F.A.R., Camara, C.A. and Silva, T.M.S. (2012). Palynological Origin, Phenolic Content and Antioxidant Properties of Honeybee-Collected Pollen from Bahia, Brazil. Molecules, 17, 1652-1664. http://dx.doi.org/10.3390/molecules17021652
[5] Feás, X., Vázquez-Tato, M.P., Estevinho, L., Seijas, J.A. and Iglesias, A. (2012) Organic Bee Pollen: Botanical Origin, Nutritional Value, Bioactive Compounds, Antioxidant Activity and Microbiological Quality. Molecules, 17, 8359-8377. http://dx.doi.org/10.3390/molecules17078359
[6] Paramás, A.M.G., Bárez, J.A.G., Marcos, C.C., García-Villanova, R.J.G. and Sanchez, J.S. (2006) HPLC-Fluorimetric Method for Analysis of Amino Acids in Products of the Hive (Honey and Bee-Pollen). Food Chemistry, 95, 148-156. http://dx.doi.org/10.1016/j.foodchem.2005.02.008
[7] Szczesna, T. (2006) Long-Chain Fatty Acids Composition of Honeybee-Collected Pollen. Journal of Apicultural Science, 50, 65-79. http:/www.jas.org.pl/jas_50_2_2006_8.pdf
[8] Qian, W.L., Khan, Z., Watson, D.G. and Fearnley, J. (2008) Analysis of Sugars in Bee Pollen and Propolis by Ligand Exchange Chromatography in Combination with Pulsed Amperometric Detection and Mass Spectrometry. Journal of Food Composition and Analysis, 21, 78-83.
http://dx.doi.org/10.1016/j.jfca.2007.07.001
[9] Medeiros, K.C.P., Figueiredo, C.A.V., Figueredo, T.B., Freire, K.R.L., Santos, F.A.R., Alcantara-Neves, N.M., Silva, T.M.S. and Piuvezam, M.R. (2008) Anti-Allergic Effect of Bee Pollen Phenolic Extract and Myricetin in Ovalbumin-Sensitized Mice. Journal of Ethnopharmacology, 119, 41-46.
http://dx.doi.org/10.1016/j.jep.2008.05.036
[10] Maruyama, H., Sakamoto, T., Araki, Y. and Hara, H. (2010) Anti-Inflammatory Effect of Bee Pollen Ethanol Extract from Cistus sp of Spanish on Carrageenan-Induced Rat Hind Paw Edema. BMC Complementary and Alternative Medicine, 10, 10-30.
http://www.biomedcentral.com/1472-6882/10/30
[11] Almeida-Da-Silva, I.A., Silva, T.M.S., Camara, C.A., Queiroz, N., Magnani, M., Novais, J.S., Soledade, L.E.B., Lima, E.O., De Souza, A.L. and De Souza, A.G. (2013) Phenolic Profile, Antioxidant Activity and Palynological Analysis of Stingless Bee Honey from Amazonas, Northern Brazil. Food Chemistry, 141, 3552-3558. http://dx.doi.org/10.1016/j.foodchem.2013.06.072
[12] Silva, T.M.S., Santos, F.P., Rodrigues, A.E., Silva, E.M.S., Silva, G.S., Novais, J.S., Santos, F.A.R. and Camara, C.A. (2013) Phenolic Compounds, Melissopalynological, Physicochemical Analysis and Antioxidant Activity of Jandaira (Melipona subnitida) Honey. Journal of Food Composition and Analysis, 29, 10-18. http://dx.doi.org/10.1016/j.jfca.2012.08.010
[13] Souza, S.A., Camara, C.A., Silva, E.M.S. and Silva, T.M.S. (2013) Composition and Antioxidant Activity of Geopropolis Collected by Melipona subnitida (Jandaíra) Bees. Evidence-Based Complementary and Alternative Medicine, 2013, 1-5. http://dx.doi.org/10.1155/2013/801383
[14] Erdtman, G. (1960). The Acetolysis Method. A Revised Description. Svensk Botanisk Tidskrift, 54, 561-564.
[15] Maia-Silva, C., Silva, C.I., Hrncir, M., Queiroz, R.T. and Imperatriz-Fonseca, V.L. (2012) Guia de plantas visitadas por abelhas na caatinga. Fundacao Brasil Cidadao, Fortaleza.
http://www.mma.gov.br/estruturas/203/_arquivos/livro_203.pdf
[16] Ortiz, M.E., Formaguera, M.J., Raya, R.R. and Mozzi, F. (2012) Lactobacillus reuteri CRL 1101 Highly Produces Mannitol from Sugarcane Molasses as Carbon Source. Applied Microbiology and Biotechnology, 95, 991-999. http://dx.doi.org/10.1007/s00253-012-3945-z
[17] Yang, K., Wu, D., Ye, X., Liu, D., Chen, J. and Sun, P. (2013) Characterization of Chemical Composition of Bee Pollen in China. Journal of Agricultural and Food Chemistry, 61, 708-718.
http://dx.doi.org/10.1021/jf304056b
[18] Day, S., Beyer, R., Mercer, A. and Ogden, S. (1990) The Nutrient Composition of Honey Bee-Collected Pollen in Otago, New Zealand. Journal of Apicultural Research, 29, 138-146.
[19] Szczesna, T. (2007) Concentration of Selected Elements in Honeybee-Collected Pollen. Journal of Apicultural Science, 51, 5-13. http://www.jas.org.pl/jas_51_1_2007_1.pdf
[20] Serra-Bonvehí, J. and Escolà-Jordà, R. (1997) Nutrient Composition and Microbiological Quality of Honey Bee-Collected Pollen in Spain. Journal of Agricultural and Food Chemistry, 45, 725-732. http://dx.doi.org/10.1021/jf960265q
[21] Morgano, M.A., Martins, M.C.T., Rabonato, L.C., Milani, R.F., Yotsuyanagia, K. and Rodriguez-Amaya, D.B.A. (2012) A Comprehensive Investigation of the Mineral Composition of Brazilian Bee Pollen: Geographic and Seasonal Variations and Contribution to Human Diet. Journal of the Brazilian Chemical Society, 23, 727-736. http://dx.doi.org/10.1590/S0103-50532012000400019

  
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