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Assemblages of Giardia duodenalis Isolates from Dogs by Amplification and Restriction of tpi and β-Giardin Genes and Sequencing of SSU rDNA Gene

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DOI: 10.4236/ojvm.2013.33035    3,298 Downloads   5,153 Views   Citations

ABSTRACT

Giardia duodenalis exhibits seven assemblages (A-G) that are distributed in different hosts. The A and B assemblages are commonly found in humans and several mammals, while C and D assemblages are typically found in dogs. The purpose of this study was to determine the assemblage of Giardia duodenalis present in the stool samples of ten canines using an assay based on PCR amplification, restriction analysis, and sequencing of the small subunit ribosomal DNA (SSU-rDNA), β-giardin, and triosephosphate isomerase (tpi) genes in order to establish the similarities or differences between the assemblage obtained with each gene. The results indicated that all positive isolates belonged to assemblage A, and specifically to the sub-assemblage A-I. A comparison of the SSU-rDNA gene sequence revealed the presence of three subgroups of assemblage A. These findings highlight the importance of canine transmission of Giardia in Mexico and its genetic plasticity. They also establish a method for additional and more molecularly extensive epidemiological studies to improve sanitation and hygiene in the most affected areas.

Conflicts of Interest

The authors declare no conflicts of interest.

Cite this paper

L. Eligio-Garcia, C. Adrian, C. Apolinar and J. Enedina, "Assemblages of Giardia duodenalis Isolates from Dogs by Amplification and Restriction of tpi and β-Giardin Genes and Sequencing of SSU rDNA Gene," Open Journal of Veterinary Medicine, Vol. 3 No. 3, 2013, pp. 222-227. doi: 10.4236/ojvm.2013.33035.

References

[1] R. D. Adam, “Biology of Giardia lamblia,” Clinical Microbiology Review, Vol. 14, No. 3, 2001, pp. 447-475. doi:10.1128/CMR.14.3.447-475.2001
[2] R. Cedillo-Rivera, Y. A. Leal, L. Yepez-Mulia, A. Gomez-Delgado, G. Ortega-Pierres, R. Tapia-Conyer and O. Munoz, “Seroepidemiology of Giardiasis in Mexico,” American Journal of Tropical Medicine and Hygiene, Vol. 80, No. 1, 2009, pp. 6-10.
[3] M. Gamboa, J. Basualdo, M. Cordoba, B. Pezzani, M. Minvielle and H. Lahitte, “Distribution of Intestinal Parasitoses in Relation to Environmental and Sociocultural Parameters in La Plata, Argentina,” Journal of Helminthology, Vol. 77, No. 1, 2003, pp. 15-20. doi:10.1079/JOH2002142
[4] G. L. Paniagua, E. Monroy, O. Garcia-Gonzalez, J. Alonso, E. Negrete and S. Vaca, “Two or More Enteropathogens Are Associated with Diarrhoea in Mexican Children,” Annals of Clinical Microbiology and Antimicrobials, Vol. 28, No. 1, 2007, pp. 6-17.
[5] R. C. Thompson, J. A. Reynoldson and A. H. Mendis, “Giardia and Giardiasis,” Advances in Parasitology, Vol. 32, No. 1, 1993, pp. 71-160. doi:10.1016/S0065-308X(08)60207-9
[6] V. Mircean, A. Gyorke and V. Cozma, “Prevalence and Risk Factors of Giardia duodenalis in Dogs from Romania,” Veterinary Parasitology, Vol. 184, No. 2-4, 2012, pp. 325-329. doi:10.1016/j.vetpar.2011.08.022
[7] E. Jiménez-Cardoso, L. Eligio-García, A. Cortes-Campos, A. Cano-Estrada, M. Pinto-Sagahon and C. Noguera-Estrada, “The Frequency of Intestinal Parasites in Puppies from Mexican Kennels,” Health, Vol. 2, No. 11, 2010, pp. 1317-1320. doi:10.4236/health.2010.211196
[8] C. S. Barr and D. D. Bowman, “Giardiasis,” Selections Veterinary, Vol. 3, No. 1, 1995, pp. 43-48.
[9] R. Shukla, P. Giraldo, A. Kraliz, M. Finnigan and A. L. Sanchez, “Cryptosporidium spp. and Other Zoonotic Enteric Parasites in a Sample of Domestic Dogs and Cats in the Niagara Region of Ontario, Can,” Veterinary Journal, Vol. 47, No. 1, 2006, pp. 1179-1184.
[10] J. López, K. Abarca, P. Paredes and E. Inzunza, “Intestinal Parasites in Dogs and Cats with Gastrointestinal Symptoms in Santiago, Chile,” Revista Medica Chilena, Vol. 134, No. 1, 2006, pp. 193-200.
[11] M. Lalle, E. Jimenez-Cardosa, S. M. Cacciò and E. Pozio, “Genotyping of Giardia duodenalis from Humans and Dogs from Mexico Using a Beta-Giardin Nested Polymerase Chain Reaction Assay,” Journal of Parasitology, Vol. 91, No. 1, 2005, pp. 203-205. doi:10.1645/GE-293R
[12] C. N. Macpherson, “Human Behaviour and the Epidemiology of Parasitic Zoonoses,” International Journal for Parasitology, Vol. 35, No. 11-12, 2005, pp. 1319-1331. doi:10.1016/j.ijpara.2005.06.004
[13] S. M. Cacciò and U. Ryan, “Molecular Epidemiology of Giardiasis,” Molecular and Biochemical Parasitology, Vol. 160, No. 2, 2008, pp. 75-80. doi:10.1016/j.molbiopara.2008.04.006
[14] P. T. Monis, R. H. Andrews, G. Mayrhofer and P. L. Ey, “Molecular Systematics of the Parasitic Protozoan Giardia intestinalis,” Molecular Biology and Evolution, Vol. 16, No. 9, 1999, pp. 1135-1144. doi:10.1093/oxfordjournals.molbev.a026204
[15] M. A. Cooper, C. R. Sterling, R. H. Gilman, V. Cama, Y. Ortega and R. D. Adam, “Molecular Analysis of Household Transmission of Giardia lamblia in a Region of High Endemicity in Peru,” The Journal of Infectious Diseases, Vol. 202, No. 11, 2010, pp. 1713-1721. doi:10.1086/657142
[16] J. Jerlstrom-Hultqvist, O. Franzén, J. Ankarklev, F. Xu, E. Nohynková, J. O. Andersson, S. G. Svard and B. Andersson, “Genome Analysis and Comparative Genomics of a Giardia intestinalis Assemblage E Isolate,” BMC Genomics, Vol. 11, No. 1, 2010, p. 543. doi:10.1186/1471-2164-11-543
[17] L. R. Ballweber, L. Xiao, D. D. Bowman, G. Kahn and V. A. Cama, “Giardiasis in Dogs and Cats: Update on Epidemiology and Public Health Significance,” Trends in Parasitology, Vol. 26, No. 4, 2010, pp. 180-189. doi:10.1016/j.pt.2010.02.005
[18] E. C. Faust, J. S. D′antonio, V. Odom, M. J. Miller, C. Peres, W. Sawitz, L. F. Thomen, J. Tobie and J. H. Walker, “A Critical Study of Clinical Laboratory Techniques for the Diagnosis of the Protozoan Cysts and Helminthes Egg in Feces,” American Journal of Tropical Medicine and Hygiene, Vol. 18, No. 1, 1938, pp. 169-183.
[19] M. Lalle, E. Pozio, G. Capelli, F. Bruschi, D. Crotti and S. M. Cacciò, “Genetic Heterogeneity at the Beta-Giardin Locus among Human and Animal Isolates of Giardia duodenalis and Identification of Potentially Zoonotic Subgenotypes,” International Journal of Parasitology, Vol. 35, No. 2, 2005, pp. 207-213. doi:10.1016/j.ijpara.2004.10.022
[20] C. F. Amar, P. H. Dear and J. McLauchlin, “Detection and Genotyping by Real-Time PCR/RFLP Analyses of Giardia duodenalis from Human Feces,” Journal of Medical Microbiology, Vol. 52, No. 1, 2003, pp. 681-683. doi:10.1099/jmm.0.05193-0
[21] N. Molina, D. Polverino, M. Minvielle and J. Basualdo, “PCR Amplification of Triosephosphate Isomerase Gene of Giardia lamblia in Formalin-Fixed Feces,” Revista Latinoamericana de Microbiologia, Vol. 49, No. 1-2, 2007, pp. 6-11.
[22] H. van Keulen, P. T. Macechko, S. Wade, S. Schaaf, P. M. Wallis and S. L. Erlandsen, “Presence of Human Giardia in Domestic, Farm and Wild Animals, and Environmental Samples Suggests a Zoonotic Potential for Giardiasis,” Veterinary and Parasitology, Vol. 108, No. 2, 2002, pp. 97-107. doi:10.1016/S0304-4017(02)00181-4
[23] K. Tamura, D. Peterson, N. Peterson, G. Stecher, M. Nei and S. Kumar, “MEGA5: Molecular Evolutionary Genetics Analysis Using Maximum Likelihood, Evolutionary Distance, and Maximum Parsimony Methods,” Molecular Biology and Evolution, Vol. 28, No. 1, 2011, pp. 2731-2739. doi:10.1093/molbev/msr121
[24] C. H. Liang, J. C. Tsaihong, Y. Y. Cheng and S. Y. Peng, “Occurrence and Genotype of Giardia Cysts Isolated from Faecal Samples of Children and Dogs and from Drinking Water Samples in an Aboriginal Area of Central Taiwan,” Experimental Parasitology, Vol. 131, No. 2, 2012, pp. 204-209. doi:10.1016/j.exppara.2012.04.002
[25] R. Beck, H. Sprong, E. Pozio and S. M. Cacciò, “Genotyping Giardia duodenalis Isolates from Dogs: Lessons from a Multilocus Sequence Typing Study,” Zoonotic Diseases, Vol. 12, No. 3, 2012, pp. 206-213. doi:10.1089/vbz.2011.0751
[26] R. M. McDowall, A. S. Peregrine, E. K. Leonard, C. Lacombe, M. Lake, A. R. Rebelo and H. Y. Cai, “Evaluation of the Zoonotic Potential of Giardia duodenalis in Fecal Samples from Dogs and Cats in Ontario. Can,” Veterinary Journal, Vol. 52, No. 12, 2012, pp. 1329-1333.
[27] F. Berrilli, R. D’Alfonso, A. Giangaspero, M. Marangi, O. Brandonisio, Y. Kaboré, C. Glé, C. Cianfanelli, R. Lauro and D. Di Cave, “Giardia duodenalis Genotypes and Cryptosporidium Species in Humans and Domestic Animals in Cote d’Ivoire: Occurrence and Evidence for Environmental Contamination,” Transactions of the Royal Society Tropical Medicine and Hygiene, Vol. 106, No. 3, 2012, pp. 191-195. doi:10.1016/j.trstmh.2011.12.005
[28] J. Li, P. Zhang, P. Wang, M. Alsarakibi, H. Zhu, Y. Liu, X. Meng, J. Li, J. Guo and G. Li, “Genotype Identification and Prevalence of Giardia duodenalis in Pet Dogs of Guangzhou, Southern China,” Veterinary Parasitology, Vol. 188, No. 3-4, 2012, pp. 368-371.
[29] S. M. Cacciò, M. De Giacomo and E. Pozio, “Sequence Analysis of the Beta-Giardin Gene and Development of a Polymerase Chain Reaction-Restriction Fragment Length Polymorphism Assay to Genotype Giardia duodenalis Cysts from Human Faecal Samples,” International Journal of Parasitology, Vol. 32, No. 1, 2002, pp. 1023-1030. doi:10.1016/S0020-7519(02)00068-1
[30] J. Bonhomme, L. Le Goff, V. Lemée, G. Gargala, J. J. Ballet and L. Favennec, “Limitations of tpi and β-Giardin Genes Sub-Genotyping for Characterization of Human Giardia duodenalis Isolates,” Parasitology International, Vol. 60, No. 3, 2011, pp. 327-330. doi:10.1016/j.parint.2011.05.004
[31] A. V. Scorza, L. R. Ballweber, S. Tangtrongsup, C. Panuska and M. R. Lappin, “Comparisons of Mammalian Giardia duodenalis Assemblages Based on the β-Giardin, Glutamate Dehydrogenase and Triosephosphate Isomerase Genes,” Veterinary and Parasitology, Vol. 189, No. 2-4, 2012, pp. 182-188. doi:10.1016/j.vetpar.2012.04.032
[32] L. Eligio-García, A. Cortes-Campos and E. Jiménez-Cardoso, “Genotype of Giardia intestinalis Isolates from Children and Dogs and Its Relationship to Host Origin,” Parasitology Research, Vol. 97, No. 1, 2005, pp. 1-6. doi:10.1007/s00436-005-1368-9

  
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