TITLE:
Assemblages of Giardia duodenalis Isolates from Dogs by Amplification and Restriction of tpi and β-Giardin Genes and Sequencing of SSU rDNA Gene
AUTHORS:
Leticia Eligio-Garcia, Cortés-Campos Adrian, Cano-Estrada Apolinar, Jiménez-Cardoso Enedina
KEYWORDS:
Giardia duodenalis; Genotyping; Zoonosis; tpi; β-Giardin; SSU rDNA
JOURNAL NAME:
Open Journal of Veterinary Medicine,
Vol.3 No.3,
July
11,
2013
ABSTRACT:
Giardia duodenalis exhibits seven assemblages (A-G) that are distributed in different
hosts. The A and B assemblages are commonly found in humans and several
mammals, while C and D assemblages are typically found in dogs. The purpose of
this study was to determine the assemblage of Giardia duodenalis present
in the stool samples of ten canines using an assay based on PCR amplification,
restriction analysis, and sequencing of the small subunit ribosomal DNA
(SSU-rDNA), β-giardin, and
triosephosphate isomerase (tpi) genes in order to establish the similarities or
differences between the assemblage obtained with each gene. The results
indicated that all positive isolates belonged to assemblage A, and specifically
to the sub-assemblage A-I. A comparison of the SSU-rDNA gene sequence revealed
the presence of three subgroups of assemblage A. These findings highlight the
importance of canine transmission of Giardia in Mexico and its genetic plasticity.
They also establish a method for additional and more molecularly extensive
epidemiological studies to improve sanitation and hygiene in the most affected
areas.