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Dodecylamine Derivative of Hydroxocobalamin Acts as a Potent Inhibitor of Cobalamin-Dependent Methionine Synthase in Mammalian Cultured COS-7 Cells

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DOI: 10.4236/fns.2014.514143    2,347 Downloads   2,647 Views   Citations


We evaluated whether the dodecylamine derivative of hydroxocobalamin acts as a potent inhibitor of cobalamin-dependent enzymes in an African green monkey kidney cell, COS-7. When the dodecylamine derivative (1.0 μmol/L) did not show any cytotoxicity in the cultured cells, the derivative could not affect methylmalonyl-CoA mutase (holo-enzyme) activity, but significantly inhibit methionine synthase (holo-enzyme) activity in the cell homogenates of COS-7 grown in 1.0 μmol/L hydroxocobalamin-supplemented medium. An immunoblot analysis indicated that the dodecylamine derivative could not decrease the protein level of methionine synthase, but significantly inhibit the enzyme activity.

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The authors declare no conflicts of interest.

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Bito, T. , Yasui, M. , Iwaki, T. , Yabuta, Y. , Ichiyanagi, T. , Yamaji, R. , Nakano, Y. , Inui, H. and Watanabe, F. (2014) Dodecylamine Derivative of Hydroxocobalamin Acts as a Potent Inhibitor of Cobalamin-Dependent Methionine Synthase in Mammalian Cultured COS-7 Cells. Food and Nutrition Sciences, 5, 1318-1325. doi: 10.4236/fns.2014.514143.


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