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Dodecylamine Derivative of Hydroxocobalamin Acts as a Potent Inhibitor of Cobalamin-Dependent Methionine Synthase in Mammalian Cultured COS-7 Cells

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DOI: 10.4236/fns.2014.514143    2,347 Downloads   2,647 Views   Citations

ABSTRACT

We evaluated whether the dodecylamine derivative of hydroxocobalamin acts as a potent inhibitor of cobalamin-dependent enzymes in an African green monkey kidney cell, COS-7. When the dodecylamine derivative (1.0 μmol/L) did not show any cytotoxicity in the cultured cells, the derivative could not affect methylmalonyl-CoA mutase (holo-enzyme) activity, but significantly inhibit methionine synthase (holo-enzyme) activity in the cell homogenates of COS-7 grown in 1.0 μmol/L hydroxocobalamin-supplemented medium. An immunoblot analysis indicated that the dodecylamine derivative could not decrease the protein level of methionine synthase, but significantly inhibit the enzyme activity.

Conflicts of Interest

The authors declare no conflicts of interest.

Cite this paper

Bito, T. , Yasui, M. , Iwaki, T. , Yabuta, Y. , Ichiyanagi, T. , Yamaji, R. , Nakano, Y. , Inui, H. and Watanabe, F. (2014) Dodecylamine Derivative of Hydroxocobalamin Acts as a Potent Inhibitor of Cobalamin-Dependent Methionine Synthase in Mammalian Cultured COS-7 Cells. Food and Nutrition Sciences, 5, 1318-1325. doi: 10.4236/fns.2014.514143.

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