Polyphenols, Ascorbic Acid and Carotenoids Contents and Antioxidant Properties of Habanero Pepper (Capsicum chinense) Fruit

Maira Rubi Segura Campos, Karen Ramírez Gómez, Yolanda Moguel Ordoñez, David Betancur Ancona

Faculty of Chemical Engineering, Autonomous University of Yucatán, Mérida, Mexico.
National Institute for Forestry, Agriculture and Livestock (INIFAP), Experimental Campus of Mocochá, Mocochá, México..

DOI: 10.4236/fns.2013.48A006                

Abstract

Their high bioactive compounds content and importance as dietary antioxidants has increased interest in Capsicum fruit. The fruit of seven Capsicum chinense Jacq. var. habanero genotypes grown in Yucatan, Mexico, were analyzed to quantify their phenolic compounds, carotenoids and ascorbic acid contents, and to measure their free radical scavenging (ABTS assay) and antioxidant activities (β-carotene/linoleic acid assay). Phenolics (20.54 to 20.75 mg/100 g sample), carotenoids (1.00 to 1.26 mg/100 g sample) and ascorbic acid contents (187.24 to 281.73 mg/100 g sample) varied between genotypes. Trolox equivalent antioxidant capacity (TEAC) ranged from 1.55 to 3.23 mM/mg sample. During the 120-min decolorization trial, antioxidant capacity decreased over time in the studied genotypes. Values ranged from 36%to 57% β-carotene bleaching during the first 30 minutes. Fruit from all seven studied genotypes are good antioxidant sources and hold promise as natural ingredients in functional foods.

Keywords

Capsicum chinense Jacq; Phenolic Compounds; Carotenoids Content; Ascorbic Acid Content; Antioxidant Activities

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1]	B. Halliwell, “Reactive Species and Antioxidants,” Redox Biology Is a Fundamental Theme of Aerobic Life,” Plant Physiology, Vol. 141, No. 2, 2006, pp. 312-322. doi:10.1104/pp.106.077073
[2]	G. Oboh and J. B. T. Rocha, “Distribution and Antioxidant Activity of Polyphenols in Ripe and Unripe Tree Pepper (Capsicum pubescens),” Journal of Food Biochemistry, Vol. 31, No. 4, 2007, pp. 456-473. doi:10.1111/j.1745-4514.2007.00123.x
[3]	B. C. Adedayo, G. Oboh and A. A. Akindahunsi, “Changes in the Total Phenol Content and Antioxidant Properties of Pepperfruit (Dennettiatripetala) with Ripening,” African Journal of Food Science, Vol. 4, No. 6, 2010, pp. 403409.
[4]	O. T. Ademoyegun, T. A. Fariyike and R. B. AminuTaiwo, “Effects of Poultry Droppings on the Biologically Active Compounds in Capsicum annum L. (var. Nsukka yellow),” Agriculture and Biology Journal of North America, Vol. 2, No. 4, 2011, pp. 665-672. doi:10.5251/abjna.2011.2.4.665.672
[5]	G. Antonious, L. Lobel, T. Kochhar, T. Berke and R. Jarret, “Antioxidants in Capsicum chinense: Variation among Countries of Origin,” Journal of Environmental Science and Health, Part B, Vol. 44, No. 6, 2009, pp. 621-626. doi:10.1080/03601230903000727
[6]	C. O. Perera and G. M. Yen, “Functional Properties of Carotenoids in Human Health,” International Journal of Food Properties, Vol. 10, No. 2, 2007, pp. 201-230. doi:10.1080/10942910601045271
[7]	Y. J. Surh and S. K. Seoul, “Anti-Tumor Promoting Potential of Selected Spice Ingredients with Oxidative and Anti-Inflammatory Activities,” Food and Chemical Toxicology, Vol. 40, No. 8, 2002, pp. 1091-1097. doi:10.1016/S0278-6915(02)00037-6
[8]	K. A. Naidu, “Vitamin C in Human Health and Disease Is Still a Mystery? An Overview,” Nutrition Journal, Vol. 2, 2003, pp. 2-7. doi:10.1186/1475-2891-2-7
[9]	M. Nadeem, F. Muhammad Anjum, M. Rafiq Khan, M. Saeed and A. Riaz, “Antioxidant Potential of Bell Pepper (Capsicum annum L.) a Review,” Pakistan Journal of Food Science, Vol. 21, No. 1-4, 2011, pp. 45-51.
[10]	E. Cázares-Sánchez, P. Ramírez-Vallejo, F. Castillo-González, M. Soto-Hernández, M. T. Rodríguez-González and J. L. Chávez-Servia, “Capsaicinoids and Preference of Use in Different Morphotypes of Chili Peppers (Capsicum annum L.) of East-Central Yucatán,” Agrociencia, Vol. 39, No. 6, 2005, pp. 627-638.
[11]	A. Rodríguez-Maturino, A. Valenzuela-Solorio, R. Troncoso-Rojas, D. González-Mendoza, O. Grimaldo-Juarez, M. Aviles-Marin and L. Cervantes-Diaz, “Antioxidant Activity and Bioactive Compounds of Chiltepín (Capsicum annum var. Glabriusculum) and Habanero (Capsicum chinense): A Comparative Study,” Journal of Medicinal Plants Research, Vol. 6, No. 9, 2012, pp. 1758-1763.
[12]	V. L. Singleton, R. Orthofer and R. M. Lamuela-Raventos, “Analysis of Total Phenols and Other Oxidation Substrates and Antioxidants by Means of Folin-Ciocalteu Reagent,” Methods in Enzymology, Vol. 299, No. 1, 1999. pp. 152-178. doi:10.1016/S0076-6879(99)99017-1
[13]	D. B. Rodriguez-Amaya and M. Kimura, “Harvest Plus Handbook for Carotenoid Analysis,” Harvest Plus Technical Monograph 2. Washington DC and Cali, Colombia. International Food Policy Research Institute (IFPRI) and International Center for Tropical Agriculture (CIAT), 2004.
[14]	AOAC, “Official Methods of Analysis of the Association of Official Analytical Chemists,” 15th Edition, Association of Official Analytical Chemists, Arlington, 1990, pp. 1058-1059.
[15]	A. Pukalskas, T. Van Beek, R. Venskutonis, J. Linssen, A. Van Veldhuizen and A. Groot, “Identification of Radical Scavengers in Sweet Grass (Hierochloeodorata),” Journal of Agricultural and Food Chemistry, Vol. 50, No. 10, 2002, pp. 2914-2919. doi:10.1021/jf011016r
[16]	H. E. Miller, “A Simplified Method for the Evaluation of Antioxidants,” Journal of American Oil Chemists Society, Vol. 48, No. 2, 1971, pp. 91-97. doi:10.1007/BF02635693
[17]	D. Montgomery, “Diseno y Análisis de Experimentos,” Limusa-Wiley, Mexico City, 2004.
[18]	A. Marín, F. Ferreres, F. A. Tomás-Barberán and M. Gil, “Characterization and Quantitation of Antioxidant Constituents of Sweets Pepper (Capsicum annum L.),” Journal of Agricultural and Food Chemistry, Vol. 55, No. 12, 2004, pp. 3861-3869. doi:10.1021/jf0497915
[19]	D. Zhang and Y. Hamauzu, “Phenolic Compounds, Ascorbic Acid, Carotenoids and Antioxidant Properties of Green, Red and Yellow Peppers,” Journal of Food, Agriculture and Environment, Vol. 1, No. 2, 2003, pp. 22-27.
[20]	F. Menichini, R. Tundis, M. Bonesi, M. R. Loizzo, F. Conforti, G. Statti, B. De Cindio, P. J. Houghton and F. Menichini, “The Influence of Fruit Ripening on the Phytochemical Content and Biological Activity of Capsicum chinense Jacq. cv Habanero,” Food Chemistry, Vol. 114, No. 2, 2009, pp. 553-560. doi:10.1016/j.foodchem.2008.09.086
[21]	R. Tundis, M. R. Loizzo, F. Menichini, M. Bonesi, F. Conforti, G. Statti, D. De Luca, B. De Cindio and F. Menichini, “Comparative Study on the Chemical Composition Antioxidant Properties and Hypoglycaemic Activities of Two Capsicum Annum L. Cultivars (Acuminatum Small and Cerasiferum),” Plant Foods for Human Nutrition, Vol. 66, No. 3, 2011, pp. 261-269. doi:10.1007/s11130-011-0248-y
[22]	C. Kevers, M. Falkowski, J. Tabart, J. O. Defraigne, J. Dommes and J. Pincemail, “Evolution of Antioxidant Capacity during Storage of Selected Fruits and Vegetables,” Journal of Agricultural and Food Chemistry, Vol. 55, No. 21, 2007, pp. 8596-8603. doi:10.1021/jf071736j
[23]	D. Hornero-Méndez, R. Gómez-Ladrón de Guevara and M. I. Mínguez-Mosquera, “Carotenoid Biosynthesis Changes in Five Red Pepper (Capsicum annuum L.) Cultivars during Ripening. Cultivar Selection for Breeding,” Journal of Agricultural and Food Chemistry, Vol. 48, No. 9, 2000, pp. 3857-3864. doi:10.1021/jf991020r
[24]	J. L. Guil-Guerrero, C. Martínez-Guirado, M. M. Rebolloso-Fuentes and A. Carrique-Pérez, “Nutrient Composition and Antioxidant Activity of 10 Pepper (Capsicum Annuun) Varieties,” European Food Research and Technology, Vol. 224, No. 1, 2006, pp. 1-9. doi:10.1007/s00217-006-0281-5
[25]	O. Collera-Zúniga, F. G. Jiménez and R. M. Gordillo, “Comparative Study of Carotenoid Composition in Three Mexican Varieties of Capsicum annuum L.,” Food Chemistry, Vol. 90, No. 1-2, 2005, pp. 109-114. doi:10.1016/j.foodchem.2004.03.032
[26]	M. Suhaj, “Spice Antioxidants Isolation and Their Antiradical Activity: A Review,” Journal of Food Composition and Analysis, Vol. 19, No. 6-7, 2006. pp. 531-537. doi:10.1016/j.jfca.2004.11.005
[27]	E. Alvarez-Parrilla, L. De la Rosa, R. Amarowicz and F. Shahidi, “Antioxidant Activity of Fresh and Processed Jalapeno and Serrano Peppers,” Journal of Agricultural and Food Chemistry, Vol. 59, No. 1, 2011, pp. 163-173. doi:10.1021/jf103434u
[28]	R. Re, N. Pellegrini, A. Proteggente, A. Pannala, M. Yang and C. Rice-Evans, “Antioxidant Activity Applying an Improved ABTS Radical Cationde Colorization Assay,” Free Radical and Biology Medicine, Vol. 26, No. 9-10, 1999, pp. 1231-1237. doi:10.1016/S0891-5849(98)00315-3
[29]	Y. S. Velioglu, G. Mazza, L. Gao and B. D. Oomah, “Antioxidant Activity and Total Phenolics in Selected Fruits, Vegetables, and Grain Products,” Journal of Agricultural and Food Chemistry, Vol. 46, No. 10, 1998, pp. 41134117. doi:10.1021/jf9801973