TITLE:
Mutations in Caprine DGAT1 and STAT5A Genes were Associated with Milk Production Traits
AUTHORS:
Xiaopeng An, Jinxing Hou, Haibo Zhao, Chunmei Zhu, Quanmei Yan, Yuxuan Song, Jiangang Wang, Binyun Cao
KEYWORDS:
Dairy Goat; Milk Production Traits; Fat Percentage; Pedigree
JOURNAL NAME:
Engineering,
Vol.4 No.10B,
January
17,
2013
ABSTRACT: In this study, polymorphisms of the DGAT1 andSTAT5A genes were detected in 528 individuals from Xinong Saanen and Guanzhong goat breeds by PCR-RFLP, PCR-SSCP and DNA sequencing methods. Three allelic
variants were identified: DQ380250: g.407_408insC, AJ237937: g.6798C>T and g.6852C>T in both breeds. At g.407_408insC
locus, the frequencies of C1 allele were 0.79–0.85, and frequencies
of C2 allele were
0.21–0.15. At g.6852C>T locus, frequencies of C3 allele were 0.70–0.72, and frequencies of T3 allele were 0.30–0.28. Compared with goats with
C1C1 and C3C3, those with C1C2 and C3T3 genotypes had significant effects on milk yield and fat percentage (P1C1C3T3 and C1C2C3T3 had higher milk yield than those with C1C2C3C3 (P C1C2C3C3 on
milk fat percentage was the highest in comparison with other combination
genotypes (P