Mutations in Caprine DGAT1 and STAT5A Genes were Associated with Milk Production Traits

Xiaopeng An, Jinxing Hou, Haibo Zhao, Chunmei Zhu, Quanmei Yan, Yuxuan Song, Jiangang Wang, Binyun Cao

College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi P.R. China.

DOI: 10.4236/eng.2012.410B008                

Abstract

In this study, polymorphisms of the DGAT1 andSTAT5A genes were detected in 528 individuals from Xinong Saanen and Guanzhong goat breeds by PCR-RFLP, PCR-SSCP and DNA sequencing methods. Three allelic variants were identified: DQ380250: g.407_408insC, AJ237937: g.6798C>T and g.6852C>T in both breeds. At g.407_408insC locus, the frequencies of C₁ allele were 0.79–0.85, and frequencies of C₂ allele were 0.21–0.15. At g.6852C>T locus, frequencies of C₃ allele were 0.70–0.72, and frequencies of T₃ allele were 0.30–0.28. Compared with goats with C₁C₁ and C₃C₃, those with C₁C₂ and C₃T₃ genotypes had significant effects on milk yield and fat percentage (P<0.05), respectively. The result showed that does with C₁C₁C₃T₃ and C₁C₂C₃T₃ had higher milk yield than those with C₁C₂C₃C₃ (P < 0.05). In addition, the combined effect of C₁C₂C₃C₃ on milk fat percentage was the highest in comparison with other combination genotypes (P<0.05).

Keywords

Dairy Goat; Milk Production Traits; Fat Percentage; Pedigree

Conflicts of Interest

The authors declare no conflicts of interest.

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