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has been cited by the following article:

• TITLE: A Protocol for a Highly Consistent, High Level Production in Vivo of Plasmodium falciparum Oocysts and Sporozoites

  AUTHORS: Martin Looker, Andrew W. Taylor-Robinson

  KEYWORDS: Malaria, Plasmodium falciparum, Cultivation, Oocyst, Sporozoite, Infectivity

  JOURNAL NAME: Advances in Bioscience and Biotechnology, Vol.5 No.13, November 7, 2014

  ABSTRACT: Investigation of the intimate relationship between the human malaria parasite Plasmodium falciparum and its Anopheles vector requires the reliable production and isolation of successive sexual stages of the parasite from infected mosquitoes. Such an advance in propagation would benefit a range of molecular, cellular, immunochemical and transmission-blocking research studies. Parasite cultivation, mosquito rearing, infection and subsequent dissection of mosquitoes are all highly technical procedures that require both skill and experience to perform with competence. Furthermore, to produce mosquitoes of an appropriate age to infect during the short period in which parasites are viable for infection demands precise planning in order to coordinate the interacting life cycles of the parasite and vector. Here, a protocol is described for the complete development of P. falciparum within Anopheles stephensi. A very consistent, high level production in vivo of P. falciparum oocysts and sporozoites is demonstrable by dissection of the mosquito midgut and salivary glands, respectively.