TITLE:
Somatic Embryogenesis and Organogenesis for Regeneration of Endangered Multipurpose Desert Plant Leptadenia pyrotechnica Forsk. Decne in the Kingdom of Bahrain
AUTHORS:
Manal Ahmed Sadeq, Malabika Roy Pathak, Ahmed Ali Salih, Mohammed Abido, Asma Abahussain
KEYWORDS:
Endangered Plants, Ex Situ Conservation, Leptadenia pyrotechnica, Plant Regeneration, Organogenesis, Somatic Embryogenesis
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.5 No.15,
July
10,
2014
ABSTRACT:
Leptadenia pyrotechnica is an important
multipurpose endangered plant in the Kingdom of Bahrain with restricted
distribution. Nodal explants were cultured on Murashige and Skoog (MS) medium
supplemented with different concentrations and combinations of indole acetic
acid (IAA) and 6-benzylaminopurine (BAP). Initially, 80% and 60% explants
responded in direct shoot and callus initiation response respectively in
presence of 8.88 μM BAP with 5.71 μM IAA in modified MS media after two weeks of
culture. The highest frequency of plant regeneration was observed in presence
of 8.88 μM BAP with 1.14 μM IAA following organogenic pathway of
differentiation. Hundred percent callus proliferation was observed while initial
callus developed in presence of 4.44 μM BAP with 2.85 μM IAA and was
transferred in media containing 4.44 μM, 6.66 μM BAP with 2.85 μM IAA and 13.32
μM BAP with 5.71 μM IAA. The callus derived plants were regenerated following
the pathway of indirect somatic embryogenesis. The induction of somatic
embryogenesis and plant regeneration from callus was also observed in modified
MS media supplemented with 4.44 μM BAP and 2.85 μM IAA. The plant regeneration
protocol we developed for Leptadenia pyrotechnica will be very
beneficial for biodiversity conservation and environment protection of Bahrain.
Moreover, the present paper reports for the first time specifically the somatic
embryogenesis in this multipurpose desert plant Leptadenia pyrotechnica.