TITLE:
Effect of zeta potentials on bovine serum albumin adsorption on crown composite resin surfaces in vitro
AUTHORS:
Nahoko Miyake, Tadashi Miura, Toru Sato, Masao Yoshinari
KEYWORDS:
Zeta Potential; Crown Composite Resins; Bovine Serum Albumin; Adsorption; Electrostatic Interactions
JOURNAL NAME:
Journal of Biomedical Science and Engineering,
Vol.6 No.3,
March
21,
2013
ABSTRACT: We previously studied the mechanism underlying the
adsorption of oral bacteria on the surfaces of dental prosthetic materials such
as ceramics and resins in vitro. The aim of the present study was to
examine bovine serum albumin (BSA) adsorption on crown
composite resin surfaces by means of zeta potential.
We measured the zeta potentials of resins alone, BSA alone, and resins after
BSA adsorption. Eight resins were pulverized into powders (300 - 1000 nm). All
experiments were conducted in 10 mM sodium chloride solution (pH 6.5). BSA
was dissolved in 10 mM NaCl with a concentration of 2.0 × 10-5 mol/l. An adsorption assay was performed for one hour at 37°C under
continuous rotation (6 rpm). The zeta potentials of both resins and BSA were
negative, with BSA itself less negative than the resins themselves as an
absolute value (p