Share This Article:

Effect of zeta potentials on bovine serum albumin adsorption on crown composite resin surfaces in vitro

Abstract Full-Text HTML XML Download Download as PDF (Size:202KB) PP. 273-276
DOI: 10.4236/jbise.2013.63034    3,906 Downloads   5,896 Views   Citations

ABSTRACT

We previously studied the mechanism underlying the adsorption of oral bacteria on the surfaces of dental prosthetic materials such as ceramics and resins in vitro. The aim of the present study was to examine bovine serum albumin (BSA) adsorption on crown composite resin surfaces by means of zeta potential. We measured the zeta potentials of resins alone, BSA alone, and resins after BSA adsorption. Eight resins were pulverized into powders (300 - 1000 nm). All experiments were conducted in 10 mM sodium chloride solution (pH 6.5). BSA was dissolved in 10 mM NaCl with a concentration of 2.0 × 10-5 mol/l. An adsorption assay was performed for one hour at 37°C under continuous rotation (6 rpm). The zeta potentials of both resins and BSA were negative, with BSA itself less negative than the resins themselves as an absolute value (p < 0.0001). The zeta potentials of seven resin surfaces after BSA adsorption were significantly less negative than were those of the resins without BSA adsorption (p < 0.0001). Eight resins were divided into two classes based on the size of the surface potential difference between each resin and the BSA. The difference in surface potential between the resins and the BSA were small, leading to the theory that particles with identical charges repulse each other, and the amounts of adsorbed BSA on these resins might be less. On the other, when the differences between the other resins and BSA are large, so that the repulsive force between two nonidentical particles becomes zero and an attractive force might be generated, then more BSA might be adsorbed on those resins. Therefore, the zeta potentials were affected by BSA adsorption and became less negative. These results suggested that electrostatic interactions play an important role in the adsorption of BSA on resin surfaces.

Conflicts of Interest

The authors declare no conflicts of interest.

Cite this paper

Miyake, N. , Miura, T. , Sato, T. and Yoshinari, M. (2013) Effect of zeta potentials on bovine serum albumin adsorption on crown composite resin surfaces in vitro. Journal of Biomedical Science and Engineering, 6, 273-276. doi: 10.4236/jbise.2013.63034.

References

[1] Kasahara, Y. and Nigaki, T. (1998) Surface characteristic of bacteria. In: Morisaki, H., Ohshima, H. and Isobe, K. Eds., Biofilm, Science Forum, Tokyo, 72-74.
[2] Furusawa, K. (1995) Adsorption and zeta potential. In: Kitahara, F., Furusawa, K., Ozaki, M. and Ohshima, H. Eds., Zeta Potential, Scientist, Tokyo, 114-132.
[3] Nakamura, N. (1995) Oral bacterial adsorption to the materials for prosthodontics in vitro. Shikwa Gakuho, 95, 375-390.
[4] Gibbons, R.J. and Etherden, I. (1985) Albumin as a blocking agent in studies of streptococcal adsorption to experimental salivary pellicles. Infection and Immunity, 50, 592-594.
[5] Miyake, N., Sato, T. and Maki, Y. (2010) Effect zeta potential on bovine serum albumin adsorption to hydroxyapatite surface in vitro. Shikwa Gakuho, 110, 105-109.
[6] Miura, T., Miyake, N., Tanabe, K. and Yoshinari, M. (2011) Change in zeta potential with physicochemical treatment of surface of anataseform tinania particles. Journal of Oral Tissue Engineering, 9, 64-70.
[7] Weerkamp, A.H., Uyen, H.M. and Busscher, H. J. (1988) Effect of zeta potential and surface energy on bacterial adhesion to uncoated and saliva-coated human enamel and dentin. Journal of Dental Research, 67, 1483-1487. doi:10.1177/00220345880670120801
[8] Van der Mei, H.C., Meijer, S. and Busscher, H. J. (1998) Electrophoretic mobilities of protein-coated Hexadecane droplets at different pH. Journal of Colloid and Interface Science, 205, 185-190. doi:10.1006/jcis.1998.5669
[9] Kambara, M. and Norde, W. (1995) Influence of fluoride applications on some physicochemical surface properties of synthetic hydroxyapatite and human dental enamel and its consequences for protein adsorption. Caries Research, 29, 210-217. doi:10.1159/000262071
[10] Young, A., Smistad, G., Karlsen, J., R?lla, G. and Rykke, M. (1997) Zeta potentials of human enamel and crown composite resins as measured by the coulter DELSA 440. Advances in Dental Research, 11, 560-565. doi:10.1177/08959374970110042501
[11] Longsworth, L.G. and Jacobsen, C.F. (1949) An electrophoretic study of the binding of salt ions by beta-lactoglobulin and bovine serum albumin. Journal of Physical Chemistry, 53, 126-135. doi:10.1021/j150466a010
[12] Bierman, A. (1955) Electrostatic forces between nonidentical colloidal particles. Journal of Colloid Science, 10, 231-245. doi:10.1016/0095-8522(55)90036-2
[13] Morisaki, H. (2009) Surface characteristics of bacterial cells living in natural environments and its relevance with bacterial survival strategy. Proceedings of the 48th Annual Meeting of Japan Oil Chemists’ Society, Nagoya, 10-12 September 2009, 86-87.
[14] Weerkamp, A.H., Quirynen, M., Marechal, M., Van der Mei, H.C., Van Steenberghe, D. and Busscher, H. J. (1989) The role of surface free energy in vivo formation of dental plaque on human enamel and polymeric substrata. Microbial Ecology in Health and Disease, 2, 11-18. doi:10.3109/08910608909140196

  
comments powered by Disqus

Copyright © 2019 by authors and Scientific Research Publishing Inc.

Creative Commons License

This work and the related PDF file are licensed under a Creative Commons Attribution 4.0 International License.