TITLE:
High concentration but low biological activity of hepatocyte growth factor in patients with chronic renal failure
AUTHORS:
Johanna Lönn, Faisal Shahzad, Fredrik Uhlin, Torbjörn Bengtsson, Gabriel Almroth, Fariba Nayeri
KEYWORDS:
Chronic Renal Failure; Hepatocyte Growth Factor; Biological Activity; Neutrophils
JOURNAL NAME:
Advances in Bioscience and Biotechnology,
Vol.3 No.4A,
August
24,
2012
ABSTRACT: Hepatocyte growth factor (HGF) is a renotropic, antifibrotic and regenerative factor with cytoprotective effects that is produced by mesenchymal cells and shows high affinity to components of extra cellular matrix, such as heparan sulphate proteoglycan (HS-PG), in healthy. Patients with chronic renal failure (CRF) suffer from a chronic inflammatory disorder. In order to assess the underlying mechanisms for development of CRF we aimed to assess the amounts and affinity of HGF in this patient group. Elisa, western blot and surface plasmon resonance (SPR) were used to study HGF in blood samples, as well as in isolated neutrophils, in CRF patients compared to healthy controls. Patients with CRF showed higher HGF levels in serum (P P