TITLE:
Combinatorial Enzyme Approach to Convert Wheat Insoluble Arabinoxylan to Bioactive Oligosaccharides
AUTHORS:
Dominic W. S. Wong, Sarah Batt, William H. Orts
KEYWORDS:
Combinatorial Enzyme Approach, Wheat Insoluble Arabinoxylan, Bioactive Oligosaccharides
JOURNAL NAME:
Advances in Enzyme Research,
Vol.11 No.1,
March
31,
2023
ABSTRACT: Combinatorial enzyme technology was applied for the conversion of wheat insoluble arabinoxylan to oligosaccharide structural variants. The digestive products were fractionated by Bio-Gel P4 column and screened for bioactivity. One fraction pool was observed to exhibit antimicrobial property resulting in the suppression of cell growth of the test organism ATCC 8739 E. coli. It has a MIC value of 1.5% (w/v, 35°C, 20 hr) and could be useful as a new source of prebiotics or preservatives. The present results further confirm the science and useful application of combinatorial enzyme approach.