Doi, Y., Wakana, D., Takeda, H., Tanaka, E. and Hosoe, T. (2022) Production of Ergot Alkaloids by the Japanese Isolate Claviceps purpurea var. agropyri on Rice Medium. Advances in Microbiology, 12, 254-269.
https://doi.org/10.4236/aim.2022.124019

has been cited by the following article:

• TITLE: Secalonic Acid and Benzoic Acid Analogues Exhibiting Cyto toxicity against Cancer Cells Isolated from Claviceps yanagawaensis

  AUTHORS: Yuji Doi, Daigo Wakana, Satoshi Kitaoka, Hisashi Takeda, Eiji Tanaka, Tomoo Hosoe

  KEYWORDS: Claviceps, Secalonic Acid, MTT Assay

  JOURNAL NAME: Advances in Microbiology, Vol.12 No.12, December 20, 2022

  ABSTRACT: The genus Claviceps (Clavicipitaceae) is noted for producing ergot alkaloids that cause ergotism. Claviceps yanagawaensis, a parasite of Zoysia japonica (family: Poaceae), has been isolated in Japan. Bioactivity screening showed that a methanol extract from a rice culture of C. yanagawaensis was cytotoxic to cancer cells. In our search for active substances, the new secalonic acid analogues (-)-5-epi-F-7 (1) and ergochrysin C (2) and a new benzoic acid analogue, dimethyl bigutol (3), were isolated along with the known compounds 3,4-dihydroxy-5-(3-methyl-2-buten-1-yl)benzoic acid (4) and methyl veratrate (5). The structures of 1 - 3 were elucidated by NMR, MS, and circular dichroism spectroscopy. MTT assays of 1 - 5 using cancer cell lines (HepG2, HL60, HT29, PANC-1, and T98G) showed that 1 - 4 exhibited cytotoxicity against cancer cells.