TITLE:
Comparing the Effect of Different Concentration of Sulfuric Acid Differentiation Solution on Acid Fast Dyeing
AUTHORS:
Hongsen Liang, Yimei Zhang, Guangchang Dan, Bingyang Wang
KEYWORDS:
Acid-Fast Dyeing, Sulfuric Acid, Differentiation Solution, Dyeing Effect, Hydrochloric Acid
JOURNAL NAME:
Advances in Bioscience and Biotechnology,
Vol.13 No.9,
September
9,
2022
ABSTRACT: Objective: In the acid-fast staining experiment of pathological
tissues, sulfuric acid and hydrochloric acid were used to evaluate the staining
results, so as to get the best staining method. Methods: Using sulfuric
acid differentiation solution and
hydrochloric acid differentiation solution, the paraffin blocks of
pathological tissues known to contain Mycobacterium tuberculosis were compared
to evaluate the staining effect. Results: When 0.5% hydrochloric acid
differentiation solution is used and the differentiation time is 6 s, the dyeing effect is
better than that of sulfuric acid differentiation solution.