TITLE:
Chemical Characterization and Cytotoxic Activity of Antarctic Macroalgae Extracts against Colorectal Cancer
AUTHORS:
Rafaele Frassini, Yeslei Paulino da Silva, Sidnei Moura, Leonardo Zambotti Villela, Aline Paternostro Martins, Pio Colepicolo, Mutue Toyota Fujii, Nair S. Yokoya, Cláudio Martin Pereira de Pereira, Vagner Reinaldo Zingalli Bueno Pereira, João Antonio Pêgas Henriques, Mariana Roesch-Ely
KEYWORDS:
Antarctic Seaweeds, Antitumor Activity, Colorectal Cancer
JOURNAL NAME:
Advances in Biological Chemistry,
Vol.9 No.5,
October
29,
2019
ABSTRACT: Background/Aim:
Antarctic seaweeds are considered a promising source of compounds with
anticancer activity. Colorectal cancer (CRC) is one of the most incident
cancers with high mortality rates worldwide. This work aimed to characterize
chemically extracts of the Antarctic macroalgae Iridaea cordata, Cystosphaera jacquinotii and Desmarestia anceps and to evaluate the cytotoxic
effects against human colon cancer HCT 116 cell line. Materials and Methods:
The extracts were obtained by depletion using an ultrasound probe and were identified by High-Performance Liquid Chromatography (HPLC) and Gas Chromatography
coupled with Mass Spectrometry (GC-MS). Cell viability was determined by MTT
assay. Results: Hexanic and chloroform extracts of the I. cordata and the hexanic,
chloroform and methanolic extracts of D. anceps were able to inhibit growth of
colorectal cancer cells in the three different incubation times (24, 48 and 72 h).
Through GC analysis, 01 compounds were identified in the hexane extract and 02
compounds in the chloroform extract of the algae I. cordata. The hexane extract of D. anceps macroalgae presented 5
compounds, chloroform extract 10 and methanolic extract 3 respectively, with special highlight to fucosterol. Carotenoid
analysis by HPLC identified β-carotene
in all species, while zeaxanthin was present in the spectrum of I. cordata and C. jacquinotii. Fucoxanthin and violaxanthin were confirmed in the
brown seaweeds C. jacquinotii and D. anceps.
Conclusion: Extracts of macroalgae I. Cordata and D. anceps may be a source
of therapeutic agents against CRC.