TITLE:
Rapid and Facile Purification of Apolipoprotein A-I from Human Plasma Using Thermoresponsive Nanoparticles
AUTHORS:
Martin Lundqvist, Tord Berggård, Erik Hellstrand, Iseult Lynch, Kenneth A. Dawson, Sara Linse, Tommy Cedervall
KEYWORDS:
Apolipoprotein A-I, nanoparticles, selective purification
JOURNAL NAME:
Journal of Biomaterials and Nanobiotechnology,
Vol.2 No.3,
May
20,
2011
ABSTRACT: Nanoparticles can be used to purify proteins from plasma. We report here the purification of apolipoprotein A-I (apoA-I) with high specificity from human plasma using copolymeric nanoparticles. We present an optimized protocol using 50:50 NiPAM:BAM copolymer nanoparticles with thermo-responsive properties as an affinity resin. Repeated pelleting and washing of nanoparticle-captured apoA-I is achieved through temperature cycling. The protein is then eluted using urea followed by an ion exchange step for protein concentration and depletion of nanoparticles.