Rapid and Facile Purification of Apolipoprotein A-I from Human Plasma Using Thermoresponsive Nanoparticles

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DOI: 10.4236/jbnb.2011.23033   PDF   HTML     5,958 Downloads   10,646 Views   Citations

Abstract

Nanoparticles can be used to purify proteins from plasma. We report here the purification of apolipoprotein A-I (apoA-I) with high specificity from human plasma using copolymeric nanoparticles. We present an optimized protocol using 50:50 NiPAM:BAM copolymer nanoparticles with thermo-responsive properties as an affinity resin. Repeated pelleting and washing of nanoparticle-captured apoA-I is achieved through temperature cycling. The protein is then eluted using urea followed by an ion exchange step for protein concentration and depletion of nanoparticles.

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M. Lundqvist, T. Berggård, E. Hellstrand, I. Lynch, K. Dawson, S. Linse and T. Cedervall, "Rapid and Facile Purification of Apolipoprotein A-I from Human Plasma Using Thermoresponsive Nanoparticles," Journal of Biomaterials and Nanobiotechnology, Vol. 2 No. 3, 2011, pp. 258-266. doi: 10.4236/jbnb.2011.23033.

Conflicts of Interest

The authors declare no conflicts of interest.

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