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http://dx.doi.org/10.1016/S0167-8809(01)00251-1

has been cited by the following article:

• TITLE: Activity of Secondary Bacterial Metabolites in the Control of Citrus Canker

  AUTHORS: Letícia Sayuri Murate, Admilton Gonçalves de Oliveira, Allan Yukio Higashi, André Riedi Barazetti, Ane Stefano Simionato, Caroline Santos da Silva, Glenda Cavalari Simões, Igor Matheus Oliveira dos Santos, Marlon Renan Ferreira, Martha Viviana Torres Cely, Miguel Octavio Pérez Navarro, Vanessa Fogaça de Freitas, Marco Antonio Nogueira, João Carlos Palazzo de Mello, Rui Pereira Leite Jr., Galdino Andrade

  KEYWORDS: X. axonopodis pv. citri, Citrus sinensis, Antibiosis, Biological Control, Natural Antibiotic

  JOURNAL NAME: Agricultural Sciences, Vol.6 No.3, March 10, 2015

  ABSTRACT: This study investigated the protective effects of secondary bacterial metabolites, produced by Pseudomonas sp. (bacterium strain LN), on citrus canker disease caused by Xanthomonas axonopodis pv. citri (Xac 306). The LN bacteria strain was cultured in liquid medium and the supernatant free-cells was treated with methanol (AMF) and ethyl acetate (AEF), respectively, and then the extract was concentrated, filtrated, lyophilized and fractionated by vacuum liquid chromatography (VLC). After VLC, eight fractions were obtained. All fractions’ activity against Xac 306 by agar well diffusion assay and minimum inhibitory concentration but in different concentrationswere tested. Cytotoxicity effects were observed in all fractions in 50 μg·mL-1 concentration. The comet assay demonstrated that the fractions EAF, VLC2 and VLC3 presented no genotoxic effects at tested concentrations. In plants only VLC3 showed significant results (p