TITLE:
Use of a Model of a Blood-Induced Bruise for the Evaluation of Formulations on Bruising
AUTHORS:
Sophie Robin, Carol Courderot-Masuyer, Hélène Tauzin, Sylvain Harbon, Marlène Chavagnac-Bonneville, Benoît Cadars, Eric Jourdan, Sandra Trompezinski, Philippe Humbert
KEYWORDS:
Bruise, Apigenin, Arnica, Skin Explants
JOURNAL NAME:
Journal of Cosmetics, Dermatological Sciences and Applications,
Vol.5 No.1,
February
4,
2015
ABSTRACT: Esthetic treatments can induce swelling and bruises. Thus, a treatment
that would prevent or hasten the resolution of bruising should be very useful.
Generally, the regression of bruising was conducted with patients or animals
models. So we decided firstly to develop an ex
vivo model in order to test antibruising properties of topical formulations
and secondly to evaluate a curative effect of a cream (mixture of arnica
extract and apigenin) in comparison with a positive control (vulnerary cream)
and also to estimate the preventive interest of this cream. The results showed
that the injection of 25 μl of blood into the dermis of skin fragments was
sufficient to create a model of induced-bruise. The duration of 24 hours was
chosen to compare the effects of actives on the decrease in the size of the
bruise. Joint effects of a pretreatment and a treatment of a mixture of arnica
extract and apigenin decreased significantly the area of bruising compared to
the treatment group, the control group and the positive control group. Many
topical products claim to improve bruising on their package label. Our model
can demonstrate their efficacy and determinate the best topical antibruising
formulation. The mechanism involved in anti-inflammatory activity of active
compounds of topical formulations is often not fully understood. Our
blood-induced model may bring some responses through the study of mediators of
the inflammation.