Use of a Model of a Blood-Induced Bruise for the Evaluation of Formulations on Bruising

DOI: 10.4236/jcdsa.2015.51002   PDF   HTML   XML   3,162 Downloads   3,694 Views   Citations


Esthetic treatments can induce swelling and bruises. Thus, a treatment that would prevent or hasten the resolution of bruising should be very useful. Generally, the regression of bruising was conducted with patients or animals models. So we decided firstly to develop an ex vivo model in order to test antibruising properties of topical formulations and secondly to evaluate a curative effect of a cream (mixture of arnica extract and apigenin) in comparison with a positive control (vulnerary cream) and also to estimate the preventive interest of this cream. The results showed that the injection of 25 μl of blood into the dermis of skin fragments was sufficient to create a model of induced-bruise. The duration of 24 hours was chosen to compare the effects of actives on the decrease in the size of the bruise. Joint effects of a pretreatment and a treatment of a mixture of arnica extract and apigenin decreased significantly the area of bruising compared to the treatment group, the control group and the positive control group. Many topical products claim to improve bruising on their package label. Our model can demonstrate their efficacy and determinate the best topical antibruising formulation. The mechanism involved in anti-inflammatory activity of active compounds of topical formulations is often not fully understood. Our blood-induced model may bring some responses through the study of mediators of the inflammation.

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Robin, S. , Courderot-Masuyer, C. , Tauzin, H. , Harbon, S. , Chavagnac-Bonneville, M. , Cadars, B. , Jourdan, E. , Trompezinski, S. and Humbert, P. (2015) Use of a Model of a Blood-Induced Bruise for the Evaluation of Formulations on Bruising. Journal of Cosmetics, Dermatological Sciences and Applications, 5, 7-14. doi: 10.4236/jcdsa.2015.51002.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] Langlois, N.E. and Gresham, G.A. (1991) The Ageing of Bruises: A Review and Study of the Colour Changes with Time. Forensic Science International, 50, 227-238.
[2] Stephenson, T. (1997) Ageing of Bruising in Children. Journal of the Royal Society of Medicine, 90, 312-314.
[3] Stevinson, C., Devaraj, V.S., Fountain-Barber, A. and Hawkins, S. (2003) Homeopathic Arnica for Prevention of Pain and Bruising: Randomized Placebo-Controlled Trial in Hand Surgery. Journal of the Royal Society of Medicine, 96, 60-65.
[4] Winter, C.A., Risley, E.A. and Nuss, G.W. (1962) Carrageenin-Induced Edema in Hind Paw of the Rat as an Assay for Anti-Inflammatory Drug. Proceedings of the Society for Experimental Biology and Medicine, 111, 544-547.
[5] Dimartino, M.J., Campbell Jr., G.K., Wolff, C.E. and Hanna, N. (1987) The Pharmacology of Arachidonic Acid-Induced Rat Paw Edema. Agents and Actions, 21, 303-305.
[6] Panthong, A., Kanjanapothi, D., Taesotikul, T. and Reutrakul, V. (2003) Anti-Inflammatory and Antipyretic Properties of Clerodendrum petasites S. Moore. Journal of Ethnopharmacology, 85, 151-156.
[7] Lussignoli, S., Bertani, S., Metelmann, H., et al. (1999) Effect of Traumeel S, a Homeopathic Formulation, on Blood Induced Inflammation in Rats. Complementary Therapies in Medicine, 7, 225-230.
[8] Conforti, A., Bellavite, P., Bertani, S., et al. (2007) Rat Models of Acute Inflammation: A Randomized Controlled Study on the Effects of Homeopathic Remedies. BMC Complementary and Alternative Medicine, 7, 1-10.
[9] Panés, J., Gerritsen, M.E., Anderson, D.C., et al. (1996) Apigenin Inhibits Tumor Necrosis Factor-Induced Intercellular Adhesion Molecule-1 Upregulation in Vivo. Microcirculation, 3, 279-286.
[10] Della Loggia, R., Tubaro, A., Dri, P., et al. (1986) The Role of Flavonoids in the Anti-Inflammatory Activity of Chamomilla recutita. Progress in Clinical Biological Research, 213, 481-484.
[11] Organisation for Economic Cooperation and Development (OECD) (2004) Test Guideline 427: In Vitro Method. Paris.
[12] Franz, T.J., Lehman, P.A. and Raney, S.G. (2009) Use of Excised Human Skin to Assess the Bioequivalence of Topical Products. Skin Pharmacology and Physiology, 22, 276-286.
[13] Leveque, N., Muret, P., Makki, S., Mac-Mary, S., Kantelip, J.P. and Humbert, P. (2004) Ex Vivo Cutaneous Absorption Assessment of a Stabilized Ascorbic Acid Formulation Using a Microdialysis System. Skin Pharmacology and Physiology, 17, 298-303.
[14] Courderot-Masuyer, C., Robin, S., Tauzin, H., Harbon, S., Mac-Mary, S., Guichard, A., et al. (2013) Evaluation of the Effects of Corticosteroids on Histamine Release by ex Vivo Cutaneous Microdialysis. Journal of Cosmetics, Dermatological Sciences and Applications, 3, 228-233.
[15] LyB, G., Knorre, A., Schmidt, T.J., Pahl, H.L. and Merfort, I. (1998) The Anti-Inflammatory Sesquiterpene Lactone Helenalin Inhibits the Transcription Factor NF-κB by Directly Targeting p65. Journal of Biological Chemistry, 273, 33508-33516.
[16] Wagner, S. and Merfort, I. (2003) Skin Penetration Behaviour of Sesquiterpene Lactones from Different Arnica Preparations Using a Validated GC-MSD Method. Journal of Pharmaceutical and Biomedical Analysis, 43, 32-38.
[17] Smolinski, A.T. and Pestka, J.J. (2003) Modulation of Lipopolysaccharide-Induced Proinflammatory Cytokine Production in Vitro and in Vivo by the Herbal Constituents Apigenin (Chamomile), Ginsenoside Rb1 (Ginseng) and Parthenolide (Feverfew). Food and Chemical Toxicology, 41, 1381-1390.
[18] Kim, H.R., Pham, H.T. and Ziboh, V.A. (2001) Flavonoids Differentially Inhibit Guinea Pig Epidermal Cytosolic Phospholipase A2. Prostaglandins, Leukotrienes and Essential Fatty Acids, 65, 281-286.
[19] Van Dross, R.T., Hong, X. and Pelling, J.C. (2005) Inhibition of TPA-Induced Cyclooxygenase-2 (COX-2) Expression by Apigenin through Downregulation of Akt Signal Transduction in Human Keratinocytes. Molecular Carcinogenesis, 44, 83-91.
[20] Chen, G.M., Zhang, J.Y., Hong, G.F. and Liu, H.M. (2006) Determination of Flavonoids Content for Verbena officinalis. Chinese Journal of Modern Applied Pharmacy, 3, 798-799.
[21] Chen, G.M., Zhang, J.Y., Zang, X.P. and Liu, H.M. (2006) Studies on Chemical Constituents of Flavonoid from Verbena officinals. Journal of Chinese Medicinal Materials, 29, 677-679.
[22] Duarte, C.M., Quirino, M.R., Patrocínio, M.C. and Anbinder, A.L. (2011) Effects of Chamomilla recutita (L.) on Oral Wound Healing in Rats. Medicina Oral, Patología Oral y Cirugía Bucal, 16, e716-e721.
[23] Liu, H.M., Bao, F.Y. and Yan, X.B. (2002) Studies on Chemical Constituents of Verbena officinalis. Journal of Chinese Medicinal Materials, 33, 492-494.
[24] Raso, G.M., Meli, R., Di Carlo, G., Pacilio, M. and Di Carlo, R. (2001) Inhibition of Inducible Nitric Oxide Synthase and Cyclooxygenase-2 Expression by Flavonoids in Macrophage J774A.1. Life Sciences, 68, 921-931.
[25] Liang, Y.C., Huang, Y.T., Tsai, S.H., Lin-Shiau, S.Y., Chen, C.F. and Lin, J.K. (1999) Suppression of Inducible Cyclooxygenase and Inducible Nitric Oxide Synthase by Apigenin and Related Flavonoids in Mouse Macrophages. Carcinogenesis, 20, 1945-1952.

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