TITLE:
Comparative Study of Bunt Pathogen Resistance to the Effects of Fungicides in Callus Co-Cultures Triticum aestivum with Tilletia caries
AUTHORS:
L. G. Yarullina, R. I. Kasimova, B. R. Kuluev, O. B. Surina, L. M. Yarullina, R. I. Ibragimov
KEYWORDS:
Wheat, Tilletia caries, Calli, Triadimenol, 18S RNA, Resistance to Fungicides
JOURNAL NAME:
Agricultural Sciences,
Vol.5 No.10,
August
28,
2014
ABSTRACT:
The morphophysiological and molecular-genetic parameters of T. caries isolates collected from
various fields of Southern Urals agrocenoses have been analysed. Isolate 1 of
wheat callus had a high growth rate in
vitro even in the presence of 0.1 mg/l fungicide Baitan. Isolate 2 of wheat
callus had a low growth rate whereas 0.1 mg/l Baitan significantly inhibited
its growth. Comparison of nucleotide sequences of 18S RNA gene of the two
isolates showed high level of homology between them, but a large number of
nucleotide substitutions have been found. The most characteristic excision was
five nucleotides at position 461 of the isolate 2, compared with the isolate 1.
Our results allow to assume that the environmental stress including high
pesticide concentration may cause the resistance of T. caries pathogen to fungicides.