TITLE:
Poly (N-Isopropyl Acrylamide-Co-Vanillin Acrylate) Dual Responsive Functional Copolymers for Grafting Biomolecules by Schiff’s Base Click Reaction
AUTHORS:
Momen S. A. Abdelaty, Dirk Kuckling
KEYWORDS:
Dual Responsive Polymers, Functional Polymers, Grafting, Amino Acids, Schiff’s Base, Click Reactions
JOURNAL NAME:
Open Journal of Organic Polymer Materials,
Vol.8 No.2,
April
30,
2018
ABSTRACT: This article reports on the
synthesis of acrylate monomer from renewable material. Vanillin was selected to
be the start material to produce new monomer called vanillin acrylate and
abbreviated by (VA). It has been successfully investigated by 1H, 13C
NMR, IR and UV and all results were in logic state. The next step was to
synthetize three different thermo-responsive functional copolymers by
incorporation of three different molar ratios of vanillin acrylate (10, 20, 30
mol%) with N-Isopropylacrylamide via free radical polymerization by AIBN
as initiator in solution. All copolymers were deduced by 1NMR and IR
and all showed the presence of aldehyde group. The copolymer was used for
grafting of tryptophan and β-alanine through the chemical link between amino
group and the active aldehyde group by click reactions to form Schiff’s base
imine compounds. Moreover, polymers were also elucidated by 1HNMR,
IR and UV, Size Exclusion Chromatography (SEC) was used for the molecular
weight determination, differential scanning calorimeter (DSC) for glass
temperature of solid polymers, XRD for crystallinity. UV-vis Spectroscopy was
used for the determination of phase separation or the lower critical solution
temperature (Tc) of polymers solution not only in deionized
water but in pH5 and pH11. The mount of conversation and linked amino acid was
determined by UV-vis Spectroscopy.