Effects of Addition of Lactobacillus plantarum and Enterococcus faecium Inoculants to High-Nitrogen Fertilized Timothy (Phleum pratense L.) on Fermentation, Nutritive Value, and Feed Intake of Silage


The objective of this study was to examine the effects of addition of inoculant to high-nitrogen (N) fertilized timothy on fermentation, nutritive value, and feed intake of silage. The silage of timothy cultivated with a N fertilizer rate at two levels (high level (H), standard level (S)) was prepared with (SI, HI) or without (SC, HC) an inoculants. The CP content of H increased by 38 g·kg-1 DM compared with that of S, and the WSC and ADF contents decreased compared with those of S. Regarding the fermentation of silage, the pH and NH3-N ratio was significantly lower in the silage with inoculant (SI and HI), showing improvement of the fermentation compared with those of the silage without the addition (SC and HC). The CP content was significantly higher in HC and HI than in SC and SI, and the NDF content was the lowest in HI among the four treatments. The CP digestibility of HC and HI was significantly higher than those of SC and SI. The EE digestibility of SI was significantly higher than that of SC, and that of HI was significantly higher than that of HC. The DCP contents were significantly higher in HC and HI than in SC and SI. The TDN content was the lowest in SC and highest in HI. The DCP intakes of HC and HI were significantly higher than those of SC and SI. When the N fertilizer rate was increased, the DCP content and DCP intake of the silage increased, and the addition of inoculant improved the fermentation and increased the TDN content.

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Wang, P. , Souma, K. , Okamoto, H. , Yano, T. , Nakano, M. , Furudate, A. , Sato, C. , Zhang, J. and Masuko, T. (2014) Effects of Addition of Lactobacillus plantarum and Enterococcus faecium Inoculants to High-Nitrogen Fertilized Timothy (Phleum pratense L.) on Fermentation, Nutritive Value, and Feed Intake of Silage. American Journal of Plant Sciences, 5, 3889-3897. doi: 10.4236/ajps.2014.526407.

Conflicts of Interest

The authors declare no conflicts of interest.


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