Evaluation of Phenolic Content and Antioxidant Property of Crossandra infundibuliformis Leaves Extracts


The present study was aimed to investigate the phenolic content and antioxidant property of Crossandra infundibuliformis leaves. Shade dried leaves were successively extracted and determined phenolic and flavonol content of the extracts. Six different in vitro antioxidant assays such as 1, 1’-diphenyl-2-picrylhydrazyl (DPPH), lipid peroxidation (LPO), nitric oxide, hydrogen peroxide radical scavenging, reducing power of the extracts and total antioxidant capacity (values were expressed as mg ascorbic acid (vitamin C) equivalent antioxidant capacity per gram of extracts) assays were followed to determine the antioxidant capacity of the extracts. The results of this study indicated that methanol extract possess higher level of phenolic (98.52 mg of gallic acid/g of extract) and flavonol (84.59 mg of rutin/g of extract) constituents. The antioxidant property of ethyl acetate extract was significantly higher than other extracts in DPPH method. Moreover, methanol extract has significantly higher antioxidant activity in LPO inhibition (IC50 value of 70.66 μg/ml), hydrogen peroxide (IC50 value 130.33 μg/ml) and reducing capacity of the extract (1.19) than other chloroform and ethyl acetate extracts. The strong correlation was observed for phenolic content and antioxidant activities of the extracts ensuring the involvement of phenols in antioxidant activity. However, results suggests that Crossandra infundibuliformis leaves possess antioxidant activity.

Share and Cite:

Patil, K. , Jaishree, V. and Tejaswi, H. (2014) Evaluation of Phenolic Content and Antioxidant Property of Crossandra infundibuliformis Leaves Extracts. American Journal of Plant Sciences, 5, 1133-1138. doi: 10.4236/ajps.2014.59126.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] Finkel, T. and Holbrook, N.J. (2000) Oxidants, Oxidative Stress and the Biology of Ageing. Nature, 408, 239-247.
[2] Radisky, D.C., Levy, D.D., Littlepage, L.E., Liu, H., Nelson, C.M. and Fata, J.E., et al. (2005) Rac1b and Reactive Oxygen Species Mediate MMP-3-Induced EMT and Genomic Instability. Nature, 436, 123-127.
[3] Houstis, N., Rosen, E.D. and Lander, E.S (2006) Reactive Oxygen Species Have a Causal Role in Multiple Forms of Insulin Resistance. Nature, 440, 944-948. http://dx.doi.org/10.1038/nature04634
[4] Fresquet, F., Pourageaud, F., Leblais, V., Brandes, R.P., Savineau, J.P. and Marthan, R., et al. (2006) Role of Reactive Oxygen Species and gp91phox in Endothelial Dysfunction of Pulmonary Arteries Induced by Chronic Hypoxia. British Journal of Pharmacology, 148, 714-723. http://dx.doi.org/10.1038/sj.bjp.0706779
[5] Jainu, M. and Shyamaladevi, C.S. (2005) In Vitro and in Vivo Evaluation of Free Radical Scavenging Potential of Cissus quadrangularis. African Journal Biomedical Reseach, 8, 95-99.
[6] Srinivasan, R., Chandrasekar, M.J.N. and Nanjan, M.J. and Suresh, B. (2007) Antioxidant Activity of Caesalpinia digyna Root. Journal of Ethnopharmacology, 113, 284-291. http://dx.doi.org/10.1016/j.jep.2007.06.006
[7] Jayaprakasha, G.K., Selvi, T. and Sakariah, K.K. (2003) Antibacterial and Antioxidant Activities of Grape (Vitis vinifera) Seed Extract. Food Research International, 36, 117-122. http://dx.doi.org/10.1016/S0963-9969(02)00116-3
[8] Pietta, P., Simonetti, P. and Mauri, P. (1998) Antioxidant Activity of Selected Medicinal Plants. Journal of Agriculture and Food Chemistry, 46, 4487-4490. http://dx.doi.org/10.1021/jf980310p
[9] Kirtikar, K.R. and Basu, B.D. (2001) Indian Medicinal Plants. 2nd Edition, Oriental Enterprises, Uttaranchal, Volume 8, 2604.
[10] Abhijit, D. and De, J.N. (2010) Ethnoveterinary Uses of Medicinal Plants by the Aboriginals of Purulia District, West Bengal. India Science Alert, 95, 551-559.
[11] Madhumitha, G., Saral, A.M., Senthilkumar, B. and Sivaraj, A. (2010) Hepatoprotective Potential of Petroleum Ether Leaf Extract of Crossandra infundibuliformis on CCl4 Induced Liver Toxicity in Albino Mice. Asian Pacific Journal of Tropical Medicine, 3, 788-790.
[12] Madhumitha, G. and A. M. Saral (2011) Preliminary Phytochemical Analysis, Antibacterial, Antifungal and Anticandidal Activities of Successive Extracts of Crossandra infundibuliformis. Asian Pacific Journal of Tropical Medicine, 4, 192-195.
[13] Meda, A., Lamien, C.E., Romito, M., Millogo, J. and. Nacoulma, O.G (2005) Determination of the Total Phenolic, Flavonoid and Praline Contents in Burkina Fasan Honey, As Well As Their Radical Scavenging Activity. Food Chemistry, 91, 571-577. http://dx.doi.org/10.1016/j.foodchem.2004.10.006
[14] Willet, W.C. (2002) Balancing Life-Style and Genomics Research for Disease Prevention. Science, 296, 695-698.
[15] Huong, N.T.T., Malsumato, K., Kasai, R., Yamasaki, K. and Watana, B. (1998) In Vitro Antioxidant Activity of Vietnamese ginseng saponin and Its Components. Biological and Pharmaceutical Bulletin, 21, 978-981.
[16] Blois, M.S. (1958) Antioxidant Determinations by the Use of a Stable Free Radical. Nature, 181, 1199-1200.
[17] Jaishree, V., Badami, S. and Suresh, B. (2008) In Vitro Antioxidant Activity of Enicostemma axillare. Journal of Health Science, 54, 524-528. http://dx.doi.org/10.1248/jhs.54.524
[18] Athukorala, Y., Kim, K.N. and Jeon, Y.J. (2006) Antiproliferative and Antioxidant Properties of an Enzymatic Hydrolysate from Brown Alga Ecklonia cava. Food and Chemical Toxicology, 44, 1065-1074.
[19] Hseu, Y.C., Chang, W.H., Chen, C.S., Liao, J.W., Huang, C.J., Lu, F.J., et al. (2008) Antioxidant Activities of Toona sinensis Leaves Extracts Using Different Antioxidant models. Food and Chemical Toxicology, 46, 105-114.
[20] Aoshima, H., Tsunoue, H., Koda H. and Kiso, Y. (2004) Aging of Whiskey Increases 1, 1-Diphenyl-2-Picrylhydrazyl Radical Scavenging Activity. Journal of Agriculture and Food Chemistry, 52, 5240-5244.
[21] Coyle, J.T. and Puttfarcken, P. (1993) Oxidative Stress, Glutamate and Neurodegenerative Diseases. Science, 219, 1184. http://dx.doi.org/10.1126/science.6338589
[22] Ialenti, A., Moncada, S. and Dirosa, M. (1993) Modulation of Adjuvant Arthritis by Endogenous Nitric Oxide. British Journal of Pharmacology, 110, 701. http://dx.doi.org/10.1111/j.1476-5381.1993.tb13868.x
[23] Oyaizu, M. (1986) Studies on Product of Browning Reaction Prepared from Glucose Amine. Japanese Journal of Nutrition, 44, 307-315. http://dx.doi.org/10.5264/eiyogakuzashi.44.307

Copyright © 2024 by authors and Scientific Research Publishing Inc.

Creative Commons License

This work and the related PDF file are licensed under a Creative Commons Attribution 4.0 International License.