Nanodiamond-Based Tests Systems for Biochemical Determination of Glucose and Cholesterol


Model test-systems for biochemical determination of glucose and cholesterol are designed by covalent coupling of enzymes to the surface of modified nanodiamonds (MND). In both cases after immobilization on MND the enzymes exhibit activity and catalyze the formation of a colored product in the chain of successive enzymatic reactions of oxidative azocoupling (Н2О2—4-aminoantipyrine—phenol). Produced MND-enzymes complexes: function in DI water and various buffers, over a wide range of temperature and pH, provide for the linear product yield over a broad concentration range of analyzed substances, make possible to test cholesterol and glucose in vitro many times, exhibit functional activity after long storage at + 4°C.

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N. Ronzhin, A. Baron, E. Mamaeva, A. Puzyr and V. Bondar, "Nanodiamond-Based Tests Systems for Biochemical Determination of Glucose and Cholesterol," Journal of Biomaterials and Nanobiotechnology, Vol. 4 No. 3, 2013, pp. 242-246. doi: 10.4236/jbnb.2013.43030.

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The authors declare no conflicts of interest.


[1] K. B. Holt, “Diamond at the Nanoscale: Applications of Diamond Nanoparticles from Cellular Biomarkers to Quantum Computing,” Philosophical Transactions of the Royal Society A, Vol. 365, No. 1861, 2007, pp. 2845-2861. doi:10.1098/rsta.2007.0005
[2] A. Schrand, S. A. C. Hens and O. A. Shenderova, “Nanodiamond Particles: Properties and Perspectives for Bioapplications,” Critical Reviews in Solid State and Materials Sciences, Vol. 34, No. 1-2, 2009, pp. 18-74. doi:10.1080/10408430902831987
[3] J. M. Say, C. van Vreden, D. J. Reilly, L. J. Brown, J. R. Rabeau and N. J. C. King, “Luminescent Nanodiamonds for Biomedical Applications,” Biophysical Reviews, Vol. 3, No. 4, 2011, pp. 171-184. doi:10.1007/s12551-011-0056-5
[4] V. N. Mochalin, O. Shenderova, D. Ho and Y. Gogotsi, “The Properties and Applications of Nanodiamonds,” Nature Nanotechnology, Vol. 7, No. 1, 2012, pp. 11-23. doi:10.1038/nnano.2011.209
[5] V. S. Bondar and A. P. Puzyr, “Nanodiamonds for Biological Investigations,” Physics of the Solid State, Vol. 46, No. 4, 2004, pp. 716-719. doi:10.1134/1.1711457
[6] A. P. Puzyr, A. V. Baron, K. V. Purtov, E. V. Bortnikov, N. N. Skobelev, O. A. Mogilnaya and V. S. Bondar, “Nanodiamonds with Novel Properties: A Biological Study,” Diamond and Related Materials, Vol. 16, No. 12, 2007, pp. 2124-2128. doi:10.1016/j.diamond.2007.07.025
[7] V. S. Bondar, I. O. Pozdnyakova and A. P. Puzyr, “Applications of Nanodiamonds for Separation and Purification of Proteins,” Physics of the Solid State, Vol. 46, No. 4, 2004, pp. 758-760. doi:10.1134/1.1711468
[8] K. V. Purtov, A. I. Petunin, A. E. Burov, A. P. Puzyr and V. S. Bondar, “Nanodiamonds as Carriers for Address Delivery of Biologically Active Substances,” Nanoscale Research Letters, Vol. 5, No. 3, 2010, pp. 631-636. doi:10.1007/s11671-010-9526-0
[9] A. P. Puzyr, V. S. Bondar, A. A. Bukayemsky, G. E. Selyutin and V. F. Kargin, “Physical and Chemical Properties of Modified Nanodiamonds,” NATO Science Series II: Mathematics, Physics and Chemistry, Vol. 192, 2005, pp. 261-270. doi:10.1007/1-4020-3322-2_20
[10] N. Gibson, O. Shenderova, T. J. M. Luo, S. Moseenkov, V. Bondar, A. Puzyr, K. Purtov, Z. Fitzgerald and D. W. Brenner, “Colloidal Stability of Modified Nanodiamond Particles,” Diamond and Related Materials, Vol. 18, No. 4, 2009, pp. 620-626. doi:10.1016/j.diamond.2008.10.049
[11] A. P. Puzyr and V. S. Bondar, “Method of Production of Nanodiamonds of Explosive Synthesis with an Increased Colloidal Stability,” RU Patent No. 2252192, 2003.
[12] J. Brandt, L. O. Andersson and J. Porath, “Covalent Attachment of Proteins to Polysaccharide Carriers by Means of Benzoquinone,” Biochimica et Biophysica Acta—Protein Structure, Vol. 386, No. 1, 1975, pp. 196-202. doi:10.1016/0005-2795(75)90259-7
[13] L. A. Osterman, “Chromatography of Proteins and Nucleic Acids,” Nauka, Moscow, 1985.
[14] M.-A. Mateescu, G. Weltrowska, E. Agostinelli, R. SaintAndre, M. Weltrowski and B. Mondovi, “Ready to Use PBenzoquinone-Activated Supports for Biochemical Coupling, with Special Applications for Laccase Immobilization,” Biotechnology Techniques, Vol. 3, No. 6, 1989, pp. 415-420. doi:10.1007/BF01875011
[15] A. N. Yeremin, T. V. Semashko and R. V. Mikhailova “Cooxidation of Phenol and 4-Aminoantipyrine, Catalyzed by Polymers and Co-Polymers of Horse Radish Root and Glucose Oxidase of Penicillium Funiculosum 46.1,” Applied Microbiology and Biotechnology, Vol. 42, No. 4, 2006, pp. 452-461.
[16] Official Website of the OMB Medical Company. =123

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