Preliminary Report of PGR’s Influence to Multiple Shoots Induction and Plant Regeneration on Plumbago auriculata


With the explants of young nodals and leaves of Plumbago auriculata, we studied multiple shoots induction and plant regeneration in the medium with different PGR proportions. The results showed the nodals of Plumbago auriculata could be used as the explant to induce multiple shoots. After culturing young nodals for 30 d, optimum single shoot formation was achieved on MS medium supplemented with 0.3 mg/L 6-BA and the induction rate was 70.79%. After being transferred to the medium containing 1.0 mg/L 6-BA, 1.0 mg/L NAA and 0.2 mg/L IAA for 1 - 2 generation, single shoot was induced to form multiple shoots with the multiplication rate was 506.45%. After multiple shoots grew to 3 cm high, the multiple shoots were cut into single shoots and transferred onto half-strength MS medium supplemented with 0.5 mg/L NAA and the rooted rate was 94.33%. Finally the intact plants were obtained.

Share and Cite:

Chen, Y. and Gao, S. (2013) Preliminary Report of PGR’s Influence to Multiple Shoots Induction and Plant Regeneration on Plumbago auriculata. American Journal of Plant Sciences, 4, 23-29. doi: 10.4236/ajps.2013.45A004.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] H. Qin, “Plumbago Auriculata, Rookie of the Landscape,” China Flowers & Horticulture, Vol. 24, 2010, p. 24.
[2] I. J. Crouch, J. F. Finnie and J. van Staden, “Studies on the Isolation of Plumbagin from in Vitro and in Vivo Grown Drosera Species,” Plant Cell, Tissue and Organ Culture, Vol. 21, No. 1, 1990, pp. 79-82. doi:10.1007/BF00034496
[3] Q. R. Zhang, “Research on the Chemical Composition of Plumbago zeylanica,” Traditional Chinese Medicines, Vol. 30, No. 5, 2007, pp. 558-560.
[4] J. M. Li, “Tutorial of Plant Tissue Culture,” China Agricultural University Press, Beijing, 1996.
[5] S. Chetia. “High Frequency in Vitro Shoot Multiplication of Plumbago indica, a Rare Medicinal Plant,” Phytochemistry, Vol. 62, 2003, p. 619.
[6] I. Sivanesan, “Shoot Regeneration and Somaclonal Variation from Leaf Callus Cultures of Plumbago zeylanica Linn,” Asian Journal of Plant Sciences, Vol. 6, No. 1, 2010, pp. 83-84.
[7] G. R. Rout, “Rapid Clonal Propagation of Plumbago zeylanica Linn,” Plant Growth Regulation, Vol. 28, No. 1, 1999, pp. 1-4. doi:10.1023/A:1006146713143
[8] M. Carola, “High Frequency of Plant Regeneration in Plumbago zeylanica Linn from Cotyledons Via-Somatic Embryogenesis,” Plant Cell Reports, Vol. 16, 2006, pp. 295-298.
[9] Z. X. Liu and X. H. Liao, “Technology of Plant Tissue Culture,” Chemical Industry Press, Beijing, 2007.
[10] S. C. Chen, “Plant Tissue Culture,” Chongqing University Press, Chongqing, 2006.
[11] J. F. Wang, “Tissue Culture and Rapid Propagation Technology of Flowers,” China Forestry Publishing, Beijing, 2006.
[12] X. Y. Feng, “Rapid Propagation of L. sinuatum by Tissue Culture,” Guizhou Agricultural Sciences, Vol. 30, No. 1, 2002, pp. 9-13.
[13] G. J. Tang, “Technology of Rapid Propagation of L. sinuatum,” China Flowers & Horticulture, Vol. 12, 2003, p. 16.
[14] L. L. Lu, Z. L. Li and Q. Jiang, “Rapid Propagation of L. sinuatum by Tissue Culture,” Acta Agriculturae Jiangxi, Vol. 21, No. 6, 2009, pp. 40-42.
[15] C. M. Yang, L. F. Wu and Zhang, “Selection of Virus- Free Seedling Medium of L. sinuatum,” Yunnan Agricultural Science and Technology, Vol. 1, 2005, pp. 778-784.
[16] X. F. Fan and Y. L. Yang, “Callus Induction and Plant Regeneration of Limonium aureum,” Acta Botanica Boreali-Occidentalia Sinica, Vol. 27, No. 2, 2010, pp. 0257- 0261.

Copyright © 2024 by authors and Scientific Research Publishing Inc.

Creative Commons License

This work and the related PDF file are licensed under a Creative Commons Attribution 4.0 International License.