The Presence of Bisphenol A in Thermal Paper Receipts, and Its Extraction in Three Media: Water, Sanitizers, and Artificial Perspiration (Sweat)

Abstract

Bisphenol A (BPA) is a synthetic chemical commonly found in thermal paper receipts, which are widely used in various sectors such as supermarkets, gas stations, and ATMs. BPA is known to have adverse effects on human health, including endocrine disruption, reproductive toxicity, neurobehavioral disorders, and carcinogenicity. This study aims to evaluate different extraction methods to identify the optimal ratio for recovering BPA from thermal paper receipts in the Saudi Arabian market. Using liquid chromatography-tandem mass spectrometry (LC-MS/MS), we compared six extraction solutions: distilled water, distilled water/methanol (30:70), artificial acidic sweat/methanol (50:50), artificial alkaline sweat/methanol (50:50), artificial acidic sweat, and artificial alkaline sweat. Our findings indicate that the 30:70 distilled water/methanol and 50:50 artificial alkaline sweat/methanol solutions provided the highest BPA recovery, suggesting these ratios as the most effective for BPA extraction.

Share and Cite:

Sufyani, S.M., Aljohani, A.M., Alyamani, A.S., Alshammri, M.A., Theeb, M.H., Almahmoud, S.A.J. and Alhamzani, A.G. (2026) The Presence of Bisphenol A in Thermal Paper Receipts, and Its Extraction in Three Media: Water, Sanitizers, and Artificial Perspiration (Sweat). <i>International Journal of Analytical Mass Spectrometry and Chromatography</i>, <b>14</b>, 17-32. doi: <a href='https://doi.org/10.4236/ijamsc.2026.142002' target='_blank' onclick='SetNum(151243)'>10.4236/ijamsc.2026.142002</a>.

1. Introduction

Thermal paper receipts are widely used in various sectors and settings, such as event tickets, retail, banking, healthcare, and education, to provide customers with a record of their transactions. However, these receipts may pose a potential health and environmental hazard, as they often contain Bisphenol A (BPA), 2,2-bis(4-hydroxyphenyl) propane, a synthetic chemical substance. as shown in Figure 1 [38]. There are concerns when touching these thermal papers, as Bisphenol A may leach from the paper surface and enter the human body through dermal absorption, ingestion, or inhalation [1].

Figure 1. Bisphenol A, 2,2-bis(4-hydroxyphenyl) propane, Chemical Formula: C15H16O2, M. Wt.: 228.29.

BPA is the most common color developer found in thermal paper, and it is easily released into the environment, which may cause dermal harms for those who are working in cashiers during their working hours [2]-[4]. BPA can be absorbed throughout the skin or enter the bloodstream of individuals touching their mouths after coming into contact with it [5]. More importantly, many individuals fail to recognize the potential risks associated with the transfer of substances from the thermal paper to their bodies. This concern is especially critical under conditions of humidity, sweating, or following the use of alcohol-based sanitizers.

There are four common chemicals (e. g., Lueco dyes, Developers, sanitizers, and stabilizers) that are used in the production of thermal paper. When they are combined, a clear and long-lasting image is created. The developers, such as Bisphenol A (BPA) and Bisphenol S (BPS), as well as sulfonylureas and zinc salts, are the essential components to produce the color [6]. When the dye is subjected to temperatures above its melting point within an acidic environment, the dye then becomes colored. When all of the aforementioned substances are mixed together under a heat source, the dye becomes dark. To ensure the chemical reaction is controlled and successful, sensitizers also have to be included, which are usually 3-methyl-phenoxy or 2-(benzyloxy) naphthalene, that melt around 100˚C. Sensitizers help improve the thermal reaction of the coating, facilitating dye and developer integration during melting, while stabilizers ensure smudge-free, durable prints [7].

It was reported that BPA is an endocrine disruptor that can interfere with the hormonal system and cause various adverse effects, such as reproductive dysfunction, metabolic disorders, neurodevelopmental impairment, and increased risk of cancer [8] [9]. Studies in mice show BPA exposure can result in increased prostate weight, brain and reproductive development issues, disrupted testosterone and sperm production, and reduced semen quality with DNA damage [10]. Moreover, BPA can also contaminate the environment and affect wildlife and ecosystems, as thermal paper receipts are frequently discarded and not recycled properly [11]-[12]. Therefore, the use of this substance in baby and drinking bottles was prohibited in the European Union in 2011. (Directive, 2011/8/EU) [13].

Large quantities of BPA has been used in industry for manufacturing polycarbonate plastic, epoxy resin, dental materials as well as paper industry [14]. The production of BPA reached 8000 tons as market size volume by 2023 [15]. Forty-six percent of production is in Asia, followed by Europe (⁓28%), and America (26%) [10]. Globally, BPA is a key components of the production of polycarbonate plastics used for food packaging, epoxy resins, medical devices, printing, coatings, printer inks, and flame retardants raising concerns about human exposure [16].

Thermal papers that are coated with BPA have the potential to readily transfer BPA to human skin upon contact, hence, it cannot be negligible [3]. In literature, people involved in direct handling of thermal paper are exposed to BPA at levels approximately 50 to 3366 folds higher than those not in contact with it [17]-[19]. These studies are based on a constructed layout of exposure, which may lead to conflicting observations. In another study, voluntary employees (n = 32), who are in continuous contact with thermal papers receipts for at least 2 hours, showed higher levels of BPA [20]. There is an increasing concern about BPA in receipts, in terms of its harmanes and Extraction for both human and environment, respectively. In Europe, the tolerable daily intake (TDI) levels for BPA was established to avoid health risks, therefore, risk management should keep daily human exposure below these limits [21]. Recent studies have shown that the average total amount of BPA in urine in people regularly exposed to thermal paper was 2.5 times higher than in the control group. Therefore, to avoid exposure to BPA from receipts, the European Union has set a limit of <0.02 wt% (0.2 mg/g, 200 μg/g or ng/mg) for concentrations BPA in thermal paper and this restriction is in effect, since January 2020 [22]. Some EU Member States, such as Denmark, Belgium, Sweden, and France, have implemented additional national bans on the use of BPA in food contact materials and coatings. For instance, France banned BPA from all food packaging, causing a conflict with the plastic industry [23]. The Saudi Food and Drug Authority (SFDA) has implemented regulations and standards that worn against the exceeding limits of bisphenol A in the packaging of food and beverages, emphasizing the risks associated with elevated levels [24]. Many studies have shown that there is a variation in the amounts of BPA in the thermal papers, some of which are slightly higher than the permissible percentages. Semerjian et al. [22] reported detection of BPA in thermal papers receipt samples from various locations in Sharjah, United Arab Emirates, with most samples exceeding the EU limit of 200 ng/mg. The estimated daily intakes (EDI) ranged from 8.22 × 1011 to 8.12 × 104 μg∙kg1∙bw∙day1, and for the cashiers, the study showed that EDI was between 7.89 × 10−9 and 0.0681 μg/kg bw/day. Therefore, all calculated TDIs, (4 μg/kg body weight/day), are below the European Food Safety Authority’s, while the possible daily intake of Health Canada established the provisional tolerance of 25 ug/kg body weight/day according to various documents [16]. The first UAE study [22] on BPA in thermal paper receipts, highlights the importance of BPA limits set by the EU’s and emphasizing that policies, education, and awareness can reduce dermal BPA exposure in the general and occupational populations.

Various extraction methods have been employed in previous studies to detect and quantify Bisphenol A (BPA) in thermal papers [25]. Traditional solvent extraction methods, such as those using methanol and acetonitrile, have been commonly applied due to their effectiveness in dissolving BPA [5] [26]. These organic solvents efficiently disrupt the thermal paper matrix, releasing the trapped BPA molecules.

Solid-liquid extraction procedures have been further optimized to enhance the efficiency of BPA recovery. Studies have explored solvents like distilled water and methanol, often at elevated temperatures or with sonication (ultrasound) to improve extraction efficiency [3] [27]. For instance, Liao and Kannan (2011) employed a methanol-based extraction method at 35˚C for one hour to determine BPA concentrations in thermal paper receipts, demonstrating significant recovery rates exceeding 80% [3]. Similarly, research by Hormann et al. (2014) and Biedermann et al. (2010) utilized combinations of water and organic solvents, such as water-methanol mixtures, to improve BPA extraction from thermal paper [5] [26]. These studies achieved reliable and reproducible results, highlighting the effectiveness of these optimized techniques.

Beyond traditional methods, innovative approaches like the use of artificial sweat solutions have been explored to simulate human exposure conditions more realistically [24]. Artificial perspiration, formulated to mimic both the acidic and alkaline pH of human sweat, has been used to evaluate BPA Extraction from thermal paper under conditions mimicking skin contact. These studies indicate that the pH and composition of artificial sweat can influence BPA extraction efficiency. For example, studies have shown that acidic sweat solutions may extract lower amounts of BPA compared to alkaline solutions, reflecting the potential impact of sweat pH on BPA Extraction from thermal paper [16] [21]. This research provides valuable insights into potential exposure risks associated with the everyday handling of thermal paper receipts.

This study aims to establish the best method for extracting bisphenol A (BPA) from thermal papers like receipts and tickets, considering the potential risks associated with BPA exposure and the insufficient information regarding its concentration in thermal papers within Saudi Arabia. The research will evaluate six extraction techniques to determine which one maximizes BPA recovery from thermal paper receipts, thereby determining the most effective and dependable method for subsequent BPA research in the region. This study will use LC-MS/MS as a highly sensitive and precise method, to separate and measure BPA and other substances in present thermal paper.

2. Experimental Section

2.1. Materials

Standard of bisphenol A (99% purity) as the reference material was purchased from LGC limited, Teddington, UK. Methanol, an HPLC-grade was from Sigma-Aldrich (Saint Louis, USA). High-purity deionized water (Sartorius 900Portable, Gottingen, Germany) was used for all experiments. Acidic and alkaline artificial perspirations (sweats) solutions were freshly prepared based on the procedure that used in ISO 105-E04 obtained from the Textile Products Laboratory department at Saudi Standards, Metrology, and Quality Organization (SASO).

2.2. Collection and Preparation of Samples

Eight samples of different brands of unprinted Thermal paper rolls were randomly obtained from markets in Riyadh city, Saudi Arabia. The samples were assigned numerical codes ranging from 1 to 8. The names of the brand were not revealed. The samples were cut into small pieces (0.3 to 0.5 cm) using stainless scissor to prepare them for the extraction setup.

2.3. Extraction Procedure

The solid-liquid extraction procedures were carried out into six different media of solutions as follow; 1) distilled water, 2) distilled water/methanol (30:70), 3) artificial acidic sweat/methanol (50:50), 4) artificial alkaline sweat/methanol (50:50), 5) artificial acidic sweat, and 6) artificial alkaline sweat.

Based on extraction processes that are reported in literature [28]-[30]. Using water and organic solvents like methanol, we have designed the extraction procedures by the aforementioned solvents. Our method was optimized by adding artificial perspirations as another solvent extraction, and by changing the solvents’ mixtures ratio. In addition to that, the sonication-assisted extraction and the temperature was performed for each solvent medium.

Accurately, one gram of the unprinted thermal paper samples was added into a 100 mL solution of each of the mentioned above solutions. Each mixture was shaken in a shaker for 60 minutes at room temperature. A sonication for 60 minutes at 50 Hz was employed for the extraction by using mixtures that contain methanol to validate the method. After extraction process, each mixture was filtrated using syringe filter (0.45 μm) membrane, and 10 μL aliquot was transferred into a vial for a direct detection via (LC MS/MS).

2.4. Liquid Chromatography-Mass Spectrometry

The liquid chromatography-tandem mass spectrometry (LC MS/MS) analysis was carried out using the Agilent 1260 Infinity II LC chromatographic system connected to an Agilent 6420 triple quadrupole mass spectrometer. 5 μL of samples were injected into a Zorbax Eclipse plus C18 column (Agilent, 2.1 × 100 mm 1.8-Micron) maintained at 30˚C. The mobile phase employed in the analysis consisted of 65% methanol and 35% deionized water at a flow rate of 0.3 mL/min with the following isocratic: 35% deionized water; 65% methanol. The mass spectrometer operated in a negative ion mode MRM of 227.1 to 211 (quantitative) and 227.1 to 133 (qualitative), with the electrospray ionization source directing the chromatographic column effluent to it. The ion source and MS settings were as follows: desolvation gas flow 650 L/hr, source temperature 350˚C, capillary voltage - 1000 V, and cone voltage-31V.

2.5. Standard Calibration Method

Standard stock solutions were prepared in methanol at a concentration of 100 mg·mL1. From the stock solution, standard solutions (20, 40, 80, 100, 150, 200 and 250 μg·L1 were prepared by dilution. First, the calibration range was tested at seven levels, then, six levels were used. The detection limit and quantitation were calculated based on the standard deviation (SD) of standard sample (20 mg·L1) in the blank solvent (distilled water/Methanol) in 30:70 ratio.

2.6. Validation

In order to demonstrate the consistency in the results, the method employed was validated following Saudi Standards, Metrology, and Quality Organization (SASO) guidelines. The method validation was performed in accordance with the requirements of ISO/IEC 17025:2017 to ensure the reliability and accuracy of the analytical results [39]. Parameters for validation are reported in Table 1.

Table 1. Validation parameters and reference materials analysis.

Sample Type

Mean (ppm)

SD (ppm)

RSD%

LOD (ppm)

LOQ (ppm)

Recovery%

Accuracy%

Precision

Blank Water

0.02088

0.00005

0.24%

0.000165

0.00050

Excellent

200 ppm Standard

224.075

15.894

7.09%

106.95%

106.95%

Good

The limits of detection (LOD) and quantitation (LOQ) were calculated using the standard deviation of the true blank (Blank Water), in accordance with the methodology described in 40 CFR 136 Appendix B. The following equations were applied:

LOD=3.3× ( SD blank ) Slope

LDQ=10× ( SD blank ) Slope

As a result, the LOD and LOQ values are significantly lower than 20 ppm, which means that the concentrations of 20 ppm and 40 ppm fall well within the quantitative range of the method and are not near the detection limits, contrary to what appeared in the previous version. After re-evaluating the analytical data, we identified an unintentional error during data entry. The blank values were mistakenly entered in ppb instead of ppm before performing the statistical calculations (Mean, SD, RSD%, LOD, LOQ). This unit‑conversion error resulted in unrealistically low blank values and consequently affected some statistical outputs. All blank values have now been recalculated using the correct unit (ppm), and the updated results are fully consistent with the raw data and the expected performance of the analytical instrument.

3. Results and Discussion

Calibration curve was tested for seven levels. It was done by preparing standard solutions from the standard Bisphenol A (99% purity), LGC limited, Teddington, UK. The calibration was performed using standard solutions prepared in the same extraction medium used for sample analysis, ensuring matrix matching and minimizing matrix effects. The validity of the calibration for the other extraction media was confirmed through spike recovery experiments, which showed acceptable recovery values within ±10%, demonstrating that the calibration is suitable for all extraction media used in this study (Table 2).

Table 2. The validity of the calibration for the other extraction media.

Replicate

With spike (10 ppm)

Without spike

Difference

Recovery (%)

1

210.416

200.282

10.134

2

208.741

198.875

9.866

3

212.184

202.132

10.052

Mean

210.447

200.430

10.017

100.17%

A spike recovery experiment was conducted at a fortification level of 10 ppm in the extraction medium consisting of 70% methanol and 30% water. The mean concentration of the spiked sample was 210.447, while the unspiked sample showed a mean concentration of 200.430. The difference between the two means (10.017) closely matched the nominal spike level, resulting in a calculated recovery of 100.17%. This recovery value falls well within the internationally accepted accuracy range of 80% - 120%, demonstrating that the analytical method provides reliable quantification and performs accurately within this extraction matrix.

The linearity showed a correlation coefficient of a good linearity (R2 = 0.998). The calibration curve, (Figure 2) was constructed and validated using concentration levels expressed in ppm, as this unit accurately reflects the working range of the analytical method and corresponds to the expected concentration levels in real samples. Although the raw instrument signals may appear numerically similar to ppb-scale values, the calibration standards themselves were prepared and verified on a ppm basis. Consequently, all calculations of mean response, standard deviation, %RSD, recovery, accuracy, and bias were performed relative to the true ppm concentrations.

Using ppm ensures full consistency with the method’s quantitative range, the LOD/LOQ values reported in the study, and the regulatory framework under which the method is intended to operate. The results in Table 3 demonstrate that the method provides excellent precision (%RSD 0.32% - 2.58%) and acceptable accuracy (97.24% - 103.12%) across the 20 - 200 ppm range. These findings confirm that expressing the calibration curve in ppm is scientifically justified and aligned with the analytical purpose of the method.

Three replicates of each sample were analyzed, and the BPA concentrations

Figure 2. Calibration curve of standard BPA solutions.

Table 3. Accuracy and precision of calibration curve (ppm Basis).

True concentration (ppm)

Mean response

SD

%RSD

Recovery (%)

Accuracy (%)

Bias (%)

Precision (%)

20

20.051

63.92

0.32

100.25

100.25

+0.25

0.32

100

103.123

1326.57

1.29

103.12

103.12

+3.12

1.29

200

194.478

5014.61

2.58

97.24

97.24

−2.76

2.58

were evaluated as the mean of the three replicates. Validation parameters which characterize the analytical procedure are reported in Table 3.

The precision and accuracy for the BPA are summarized in Table 3. Samples of 20, 100, and 200 ppm of standard materials (BPA) with nine replicates were analyzed under the same conditions (30:70) distilled water and methanol.

The extraction process of BPA from unprinted thermal papers rolls was done according to previous reported methods [5] [23] [24] [26]. The concentration of BPA in the eight thermal papers samples was detected using different solutions mixtures as illustrated in Figure 3.

The BPA concentrations was found to range from 2.24 to 434.4 mg∙L−1 for the samples. The Technical Regulation for Paper and Cardboard by SASO, (SASO-2934), has reported that 200 mg∙L−1 is the allowed limit of BPA in thermal papers [31]. In the solid-liquid extraction, we have noticed that the Extraction of BPA in the different mixtures varied according to each solvent’s property. Solid-liquid extraction by aqueous 70% methanol and alkaline sweat/methanol for each sample showed higher Extraction of the BPA, as extractable compound, in comparison to other solvents. This result is consistent with the reported data for BPA extraction from plastic products [32] [33] BPA, in samples number 1, 4, 5, 6, and 8, was found above the allowed limit that set by SASO, compared to other samples, (2, 3, and 7) which is detected less than the permissible limit. The average concentration of BPA in each thermal paper sample after extraction with distilled

Figure 3. Concentration of BPA in the eight-samples of thermal papers.

water, distilled water/methanol, alkaline sweat/methanol, acidic sweat/methanol, alkaline sweat, and acidic sweat are 220.15, 11.7, 25.56, 307.1, 220.1, 145.6, 17.7, and 215.7 mg∙L−1, respectively as illustrated in Figure 3.

For samples that subjected to extraction with solution containing methanol have showed significant higher extraction of BPA, which is consistent with reported data in literature [34] [35]. Interestingly, the extraction of the BPA when using both basic sweat and acidic sweat without methanol is higher than that of distilled water. This is an alert since many people deal with receipts and tickets made of thermal papers, they may be exposed to the substance, especially in hot climates such as that of Riyadh region.

When comparing the Extraction of BPA into alkaline and acidic artificial sweats, the results showed almost similar concentration of the BPA for most of the thermal paper samples, with a slight increase noted for the samples that were treated with the alkaline artificial sweat, (Figure 4). These results are attributed to the chemical composition and pH values for the artificial sweats [36]. Still it falls into the risk for people health through dermal exposure. More investigations need to be carried out about transdermal exposure in terms of temperatures and humidity. Based on this Detection, samples 2, 3, and 7 showed the lowest concentration of BPA even after heating for a period of time. This is an interesting observation, it reveals that the BPA embedded in these thermal papers, is difficult to extract unlike in other thermal papers and hence may be safer to use in humid and hot climates.

2.4. Statistical Analysis

The descriptive statistics for BPA concentration in different media extractions are shown in the Table 4. The descriptive statistics reveal that each media of extraction was tested with 8 samples, and there is notable variation in the mean BPA

Figure 4. Concentration of BPA in the alkaline sweat and acid sweat.

Table 4. Descriptive statistics for different concentration methods of BPA (mg/L).

Method of Extraction

N

Mean

Std. Deviation

95% Confidence Interval for Mean

Min

Max

Lower Bound

Upper Bound

(Method1) D. W.

8

49.21

45.59

11.09

87.33

2.25

123.35

(Method2) DW/MeOH

8

220.02

175.64

73.18

366.87

6.84

411.52

(Method3) Alkaline sweat: MeOH)

8

228.03

187.22

71.51

384.55

9.42

409.86

(Method4) Acidic sweat: MeOH)

8

137.23

137.06

22.65

251.82

3.52

345.94

(Method5) Alkaline sweat

8

157.98

137.59

42.95

273.01

6.82

434.42

(Method6) Acidic sweat

8

80.09

82.96

10.73

149.45

5.69

253.79

Total

48

145.43

145.66

103.13

187.72

2.25

434.42

concentration across the methods. Extraction with DW/MeOH mixture and Alkaline sweat/MeOH mixtures recorded the highest mean concentrations, while extraction with using distilled water and acidic sweat had the lowest. Method 3 also exhibited the highest variability in BPA concentrations, as indicated by its standard deviation, whereas Method 1 had the least variability. The 95% confidence intervals for the mean BPA concentrations provide a range within which the true mean is likely to fall, with Method 2, for example, ranging from 73.18 to 366.87 mg/L.

One-way analysis of variance (ANOVA) was employed to statistically analyze the BPA concentrations in thermal papers samples. ANOVA facilitates comparing the average contents between the different extraction methods. Data were considered statistically significant at p < 0.05. Table 5 shows the one-way ANOVA for different concentration of BPA obtained by the extraction methods.

The ANOVA analysis aimed to determine whether there are statistically significant differences in BPA concentration across the six methods of extraction. The resulting p-value is 0.069, which is slightly above the conventional significance threshold of 0.05. This indicates that the differences in BPA concentrations between the extraction methods are not statistically significant, but the result is close to the significance level, suggesting a potential trend worth further investigation.

Post-Hoc Test

Table 6 shows the multiple comparison analysis reveals for different concentration of the BPA in mg/L. The LSD (Least Significant Difference) multiple

Table 5. One-way ANOVA for different concentration methods of BPA (mg/L).

Sum of Squares

df

Mean Square

F

Sig.

Between Groups

209,107.10

5

41,821.42

2.22

0.07

Within Groups

788,089.87

42

18,764.04

Total

997,196.97

47

Table 6. Multiple comparisons using LSD for different concentrations of BPA (mg/L) [37].

(I) Method

(J) Method

Mean Difference (I-J)

Sig.

95% Confidence Interval

Lower Bound

Upper Bound

Method1

D. W.

Method 2

−170.81

0.01

−309.03

−32.59

Method 3

−178.81

0.01

−317.04

−40.59

Method 4

−88.02

0.20

−226.24

50.19

Method 5

−108.76

0.12

−246.98

29.45

Method 6

−30.88

0.65

−169.10

107.33

Method2

DW/MeOH

Method 3

−8.00

0.90

−146.22

130.21

Method 4

82.78

0.23

−55.43

221.00

Method 5

62.04

0.37

−76.17

200.26

Method 6

139.92

0.04

1.70

278.14

Method3

Alkaline sweat/MeOH

Method 4

90.79

0.19

−47.42

229.01

Method 5

70.05

0.31

−68.16

208.27

Method 6

147.93

0.03

9.71

286.15

Method4

Acidic sweat/MeOH

Method 5

−20.74

0.76

−158.96

117.47

Method 6

57.13

0.40

−81.08

195.35

Method5

Method 6

77.88

0.26

−60.33

216.10

comparison analysis reveals several important findings regarding the BPA concentration significant differences across the methods of extraction media:

  • Method 1 vs. Method 2 and Method 3: There are significant differences in BPA concentrations between Method 1 and both Method 2 and Method 3, with p-values of 0.017 and 0.012, respectively. The mean BPA concentrations for Method 2 and Method 3 are significantly higher than those for Method 1, as indicated by the negative mean differences.

  • Method 2 vs. Method 6: There is also a significant difference between Method 2 and Method 6, with a p-value of 0.047, where Method 2 has a higher mean BPA concentration.

  • Method 3 vs. Method 6: There is also a significant difference between Method 3 and Method 6, with a p-value of 0.037, where Method 3 has a higher mean BPA concentration.

4. Conclusion

In this study, eight samples of thermal paper rolls, from Saudi Arabia markets, were randomly collected and analyzed for determining the concentrations of Bisphenol A (BPA) in thermal paper receipts. The method involved solid-liquid extraction and analysis via liquid chromatography-tandem mass spectrometry (LC-MS/MS). The results of our study provide important insights into the presence and variability of BPA in thermal paper receipts and its potential implications for human health and the environment. The method of analysis was validated and statistically analyzed, and it showed good linearity, trueness, and precision. Regardless of the extraction method. For BPA in thermal paper receipts set by the Saudi Standards, Metrology and Quality Organization (SASO) and the European Union (EU) BPA concentrations vary depending on the amount and type of BPA present in the paper coating, as well as the brand and source of thermal paper receipts. It is important to conduct further studies on a wide range of samples from different sources and applications of thermal paper, such as restaurants, banks, hospitals, schools, etc. This would help assess the prevalence and diversity of BPA in thermal paper receipts and estimate exposure and risks for different segments of the population, especially those most vulnerable to the effects of BPA. The results of this study will help raise awareness of the prevalence of BPA in thermal paper receipts in the Saudi Arabian market and aid in developing policies and strategies to mitigate the potential health and environmental risks associated with this synthetic chemical. Based on our analysis, we found that BPA concentrations in thermal paper receipts varied depending on the brand and source of the receipts. The levels of BPA detected in some samples exceeded the regulatory limits and guidelines set by the Saudi Standards, Metrology and Quality Organization (SASO) and the European Union (EU). This suggests that there may be a potential health risk associated with the use of thermal paper receipts in Saudi Arabia. Further research is needed to expand the scope of this study and investigate a wider range of thermal paper receipt samples from different sources and applications, such as restaurants, banks, hospitals, schools, etc. This would help provide a more comprehensive understanding of the prevalence and diversity of BPA in thermal paper receipts in Saudi Arabia and allow for a more accurate estimation of exposure and risks.

Acknowledgements

The authors thank manager and supervisors at the Chemical and Petroleum Materials Laboratory at SASO for their support and engorgement, and the instruments laboratory staff for experimental assistance on varying parts of the current study. The authors also extend their appreciation and thanks to the Department of Chemical technology at Riyadh College of Technology and the Department of Chemistry at Imam Mohammad Ibn Saud Islamic University (IMSIU) for their academic support of this study.

Author Contributions:

Conceptualization, S. S and A. M. A.; data curation, S. M. S., A. M. A., A. S. A, and M.A.A., and M. H. T.; formal analysis, A. G. A. and S. A.J.A.; investigation, A.G.A., S. M. S., A. M. A., A. S. A, and M.A.A., and M. H. T; methodology, S.M.S, A.M.A, A.G.A; project administration, A.G.A and S.M.S.; software; writing—original draft, A.G.A and S.M.S; validation, S. M. S., A. M. A., A. S. A, S.J.A.A and M.A.A., and M. H. T writing—review and editing, A.G.A and S.A.J.A. All authors have read and agreed to the published version of the manuscript.

Data Availability Statement

The data presented in this study are available on request from the corresponding author. The data are not publicly available due to privacy and ethical reasons.

Conflicts of Interest

The authors declare no conflicts of interest regarding the publication of this paper.

References

[1] LGC Standards (n.d.) “Bisphenol A”. LGC Standards.
https://www.lgcstandards.com/ES/en/Bisphenol-A/p/DRE-C10655500
[2] Reale, E., Vernez, D. and Hopf, N.B. (2020) Skin Absorption of Bisphenol A and Its Alternatives in Thermal Paper. Annals of Work Exposures and Health, 65, 206-218.[CrossRef] [PubMed]
[3] Geens, T., Goeyens, L., Kannan, K., Neels, H. and Covaci, A. (2012) Levels of Bisphenol-A in Thermal Paper Receipts from Belgium and Estimation of Human Exposure. Science of the Total Environment, 435, 30-33.[CrossRef] [PubMed]
[4] Liao, C. and Kannan, K. (2011) Widespread Occurrence of Bisphenol a in Paper and Paper Products: Implications for Human Exposure. Environmental Science & Technology, 45, 9372-9379.[CrossRef] [PubMed]
[5] Zalko, D., Jacques, C., Duplan, H., Bruel, S. and Perdu, E. (2011) Viable Skin Efficiently Absorbs and Metabolizes Bisphenol A. Chemosphere, 82, 424-430.[CrossRef] [PubMed]
[6] Biedermann, S., Tschudin, P. and Grob, K. (2010) Transfer of Bisphenol a from Thermal Printer Paper to the Skin. Analytical and Bioanalytical Chemistry, 398, 571-576.[CrossRef] [PubMed]
[7] Koehler Paper (2026) The Structure of Conventional Thermal Paper.
https://www.koehlerpaper.com/en/products/Thermal-paper/
[8] BPC (UK) Ltd (2023) The Science behind Thermal Paper Rolls.
https://bpcuk.com/the-science-behind-thermal-paper-rolls/
[9] Human Exposure to Bisphenol A in Europe.
https://www.eea.europa.eu/publications/peoples-exposure-to-bisphenol-a
[10] Rochester, J.R. and Bolden, A.L. (2015) Bisphenol S and F: A Systematic Review and Comparison of the Hormonal Activity of Bisphenol a Substitutes. Environmental Health Perspectives, 123, 643-650.[CrossRef] [PubMed]
[11] Björnsdotter, M.K., de Boer, J. and Ballesteros-Gómez, A. (2017) Bisphenol A and Replacements in Thermal Paper: A Review. Chemosphere, 182, 691-706.[CrossRef] [PubMed]
[12] Migeot, V., Dupuis, A., Cariot, A., Albouy-Llaty, M., Pierre, F. and Rabouan, S. (2013) Bisphenol A and Its Chlorinated Derivatives in Human Colostrum. Environmental Science & Technology, 47, 13791-13797.[CrossRef] [PubMed]
[13] Ballesteros-Gómez, A., Rubio, S. and Pérez-Bendito, D. (2009) Analytical Methods for the Determination of Bisphenol a in Food. Journal of Chromatography A, 1216, 449-469.[CrossRef] [PubMed]
[14] Barroso, J. (2011) Commission Directive 2011/8/EU of 28 January 2011 Amending Directive 2002/72/EC as Regards the Restriction of Use of Bisphenol A in Plastic Infant Feeding Bottles. Official Journal of the European Union, 54, 11-14.
[15] Geens, T., Aerts, D., Berthot, C., Bourguignon, J., Goeyens, L., Lecomte, P., et al. (2012) A Review of Dietary and Non-Dietary Exposure to Bisphenol-A. Food and Chemical Toxicology, 50, 3725-3740.[CrossRef] [PubMed]
[16] Bisphenol A Market Analysis.
https://www.chemanalyst.com/industry-report/bisphenol-a-market-57
[17] Panel on Food Contact Materials, Enzymes, Flavourings and Processing Aids (CEF) (2015) Scientific Opinion on the Risks to Public Health Related to the Presence of Bisphenol a (BPA) in Foodstuffs. EFSA Journal, 13, 3978.[CrossRef]
[18] Lu, S., Chang, W., Sojinu, S.O. and Ni, H. (2013) Bisphenol A in Supermarket Receipts and Its Exposure to Human in Shenzhen, China. Chemosphere, 92, 1190-1194.[CrossRef] [PubMed]
[19] Fan, R., Zeng, B., Liu, X., Chen, C., Zhuang, Q., Wang, Y., et al. (2015) Levels of Bisphenol-A in Different Paper Products in Guangzhou, China, and Assessment of Human Exposure via Dermal Contact. Environmental Science: Processes & Impacts, 17, 667-673.[CrossRef] [PubMed]
[20] Rocha, B.A., Azevedo, L.F., Gallimberti, M., Campiglia, A.D. and Barbosa, F. (2015) High Levels of Bisphenol a and Bisphenol S in Brazilian Thermal Paper Receipts and Estimation of Daily Exposure. Journal of Toxicology and Environmental Health, Part A, 78, 1181-1188.[CrossRef] [PubMed]
[21] Ehrlich, S., Calafat, A.M., Humblet, O., Smith, T. and Hauser, R. (2014) Handling of Thermal Receipts as a Source of Exposure to Bisphenol A. JAMA, 311, 859-860.[CrossRef] [PubMed]
[22] Lambré, C., Barat Baviera, J.M., Bolognesi, C., Chesson, A., Cocconcelli, P.S., Crebelli, R., et al. (2023) Re-Evaluation of the Risks to Public Health Related to the Presence of Bisphenol a (BPA) in Foodstuffs. EFSA Journal, 21, e06857.[CrossRef] [PubMed]
[23] Semerjian, L., Alawadhi, N. and Nazer, K. (2023) Detection of Bisphenol a in Thermal Paper Receipts and Assessment of Human Exposure: A Case Study from Sharjah, United Arab Emirates. PLOS ONE, 18, e0283675.[CrossRef] [PubMed]
[24] Laaninen, T. (2018) New Rules on Bisphenol A in Food Contact Materials, At a Glance.
https://www.europarl.europa.eu/RegData/etudes/ATAG/2018/614705/EPRS_ATA(2018)614705_EN.pdf
[25] (2008) FDA Statement on Release of Bisphenol A (BPA) Subcommittee Report.
https://www.sfda.gov.sa/en/awarenessarticle/897
[26] Vinod, V., Amritha, P.S. and Harathi, P.B. (2023) A Systematic Review on Bisphenols—Sources, Health Impacts, and Analytical Determination of Bisphenol A in Aqueous Samples. Separation Science Plus, 6, Article ID: 2300072.[CrossRef]
[27] Hormann, A.M., vom Saal, F.S., Nagel, S.C., Stahlhut, R.W., Moyer, C.L., Ellersieck, M.R., et al. (2014) Holding Thermal Receipt Paper and Eating Food after Using Hand Sanitizer Results in High Serum Bioactive and Urine Total Levels of Bisphenol a (BPA). PLOS ONE, 9, e110509. [Google Scholar] [CrossRef] [PubMed]
[28] Tian, H., Bai, X. and Xu, J. (2018) Extraction of Bisphenol A in Honey Samples Using Aqueous Biphasic Systems Coupled with High-Performance Liquid Chromatography. Separation Science Plus, 1, 374-381.[CrossRef]
[29] Yalçin, M.S., Geçgel, C. and Battal, D. (2016) Determination of Bisphenol a in Thermal Paper Receipts. Journal of the Turkish Chemical Society, Section A: Chemistry, 3, 167-174.[CrossRef]
[30] Eckardt, M. and Simat, T.J. (2017) Bisphenol A and Alternatives in Thermal Paper Receipts—A German Market Analysis from 2015 to 2017. Chemosphere, 186, 1016-1025.[CrossRef] [PubMed]
[31] Russo, G., Barbato, F. and Grumetto, L. (2017) Monitoring of Bisphenol a and Bisphenol S in Thermal Paper Receipts from the Italian Market and Estimated Transdermal Human Intake: A Pilot Study. Science of the Total Environment, 599, 68-75.[CrossRef] [PubMed]
[32] International Organization for Standardization ISO (2017) ISO/IEC 17025 General Requirements for the Competence of Testing and Calibration Laboratories. 3rd Edition, Geneva.
[33] Technical Regulation for Paper and Cardboard, SASO’s Board of Directors No. (181).
https://www.saso.gov.sa/en/Laws-And-Regulations/Technical_regulations/Documents/TR-Paper-and-Cardboard.pdf
[34] Park, S., Park, S., Jeong, M., Choi, J.C. and Kim, M. (2018) Fast and Simple Determination and Exposure Assessment of Bisphenol A, Phenol, P-Tert-Butylphenol, and Diphenylcarbonate Transferred from Polycarbonate Food-Contact Materials to Food Simulants. Chemosphere, 203, 300-306.[CrossRef] [PubMed]
[35] Khan, M.R., Alammari, A.M., Aqel, A. and Azam, M. (2021) Trace Analysis of Environmental Endocrine Disrupting Contaminant Bisphenol a in Canned, Glass and Polyethylene Terephthalate Plastic Carbonated Beverages of Diverse Flavors and Origin. Food Science and Technology, 41, 210-217.[CrossRef]
[36] Sungur, Ş., Köroğlu, M. and Özkan, A. (2014) Determination of Bisphenol a Migrating from Canned Food and Beverages in Markets. Food Chemistry, 142, 87-91.[CrossRef] [PubMed]
[37] Vilarinho, F., Sendón, R., van der Kellen, A., Vaz, M.F. and Silva, A.S. (2019) Bisphenol A in Food as a Result of Its Migration from Food Packaging. Trends in Food Science & Technology, 91, 33-65.[CrossRef]
[38] Vinković, K., Vukoje, M., Rožić, M. and Galić, N. (2019) Migration of Pseudoestrogen Bisphenol a from Various Types of Paper with Thermochromic Prints to Artificial Sweat Solutions. Journal of Liquid Chromatography & Related Technologies, 43, 195-202.[CrossRef]
[39] Salkind, N. (2010) Encyclopedia of Research Design. SAGE Publications, Inc.

Copyright © 2026 by authors and Scientific Research Publishing Inc.

Creative Commons License

This work and the related PDF file are licensed under a Creative Commons Attribution 4.0 International License.