Determination of Ascorbic Acid, Rutin and Antioxidant Capacities of Wild Allium roseum from Italy ()
1. Introduction
A healthy diet, rich in natural foods may help to prevent the development of several diseases [1]. Allium species (Alliaceae) have always been used as food or as medicinal plants in human history and several investigations have demonstrated that different Allium species may be useful for the prevention of pathological conditions such as liver necrosis, diabetes type 2, obesity, hypercholesterolemia, atherosclerosis, coronary heart disease, hypertension, carcinogenesis, pulmonary damages, cataract, depression and disturbances of the gastrointestinal tract [2] - [7].
The genus Allium is an important dietary source of antioxidant phytochemical products, vitamins, and different antioxidants and enzymes, which contribute to their nutritional and therapeutic characteristics [8] [9].
The beneficial effects of garlic (Allium sativum L.), at least in part, can be attributed to its ability to enhance or maintain the total antioxidant capacity of people who include these bulbs in their diet.
In previous studies, we have demonstrated that some Allium species (Allium neapolitanum Cyr., Allium subhirsutum L., Allium roseum L.), endemic to Italian flora, have an antioxidant activity, in vitro, similar or better than A. sativum [10] [11] [12]. We have also shown the protective effect of Allium neapolitanum Cyr. in rats with liver poisoning induced by ethanol [13].
The aim of this study is to compare the data of the antioxidant status of fresh organs (bulbs, bulblets, flowers bulblets, leaves and flowers) of wild Allium roseum, with the results obtained from bulbs and leaves of Allium sativum.
Hence we measured the antioxidant enzyme’s activity (glutathione peroxidase, catalase and glutathione reductase), the levels of reduced and oxidized glutathione and the content of ascorbic acid, thiosulfinates, flavonols (rutin and quercetin) and soluble proteins in Allium roseum and in bulbs, and leaves of Allium sativum.
2. Materials and Methods
All reagents were of analytical grade. HPLC standard substance (rutin, quercetin and ascorbic acid) were purchased from Sigma-Aldrich (St. Louis, Missouri) and HPLC solvents were purchased from Merck (Darmstadt, Germany). The other standard molecules and chemicals were purchased from Sigma-Aldrich (St. Louis, Missouri). Milli-Q (Millipore-Lab, Bedford, Massachusetts) purified water was used for all analyses.
2.1. Plant Collection
Two Allium species were studied: Allium sativum L. cultivated in rural farms of Poggibonsi (Siena—latitude 43˚28'14"N and longitude 11˚08'52"E) and wild Allium roseum L. collected nearby Siena (Tuscany, Italy—latitude 43˚16'27"N and longitude 11˚18'50"E). The plants were collected respectively in March, 2017 and during their blooming time in May, 2017.
The identification of the sample of Allium roseum was performed by Dr. Ilaria Bonini and a voucher specimen (SIENA-N˚ 11052) was deposited in the Herbarium Universitatis Senensis [SIENA], Department of Life Sciences, University of Siena.
2.2. Processing of Samples
Fresh organs (bulbs, bulblets, flowers bulblets, leaves and flowers) were cleaned to remove residual impurities; subsequently each part of the plant was cut and homogenized (500 mg/ml) in MilliQ purified water or a different buffer (as described below for each parameter) using a homogenizer (T25 Basic Ultra-Turrax, Janke & Kunlel, IKA-Laboratechnik, Staufen, Germany).
Depending on the specific evaluation parameters, the homogenates were treated with opportune reagents and centrifuged to different gravities according to the several methods reported below. Subsequently, all supernatants were stored at −80˚C before starting their analytical procedures.
3. Analytical Procedures
3.1. Ascorbic Acid
The organs of plants were homogenized in phosphate buffer (pH 7.4) at 0˚C. These solutions were added to an equal volume of 10% metaphosphoric acid and immediately centrifuged at 2000 g × 10 min at 0˚C. Ascorbic acid (AA) was measured by the HPLC method [14]. The supernatants were removed and filtered (Millipore 0.45 μm, Merck, Darmstadt, Germany), and 20 µl aliquots were manually injected into the high-performance liquid chromatography column (Reverse phase C18) with UV detector at 262 nm. Results were expressed as μmoles/g of fresh and unprocessed weight.
3.2. Quercetin and Rutin
The organs of plants were homogenized in phosphate buffer (pH 7.4) at 0˚C. The samples were centrifuged at 2000 g × 10 min at 0˚C. The supernatants were removed and filtered (Millipore 0.45 μm, Merck, Darmstadt, Germany). Quercetin (Q) and rutin (R) were measured by the HPLC method [15]. Twenty µl aliquots were manually injected into the high-performance liquid chromatography column (Reverse phase C18) with UV detector at 360 nm. The eluent was (CH3OH:H2O:H3PO4 = 60:40:0.4 v/v). Results were expressed as µg/g of fresh and unprocessed weight.
3.3. Thiosulfinates
The plants’ organs were homogenized in ice-cold phosphate buffer 0.125 M (pH 7.4) and this solution was centrifuged for 10 min at 0˚C at low speed (2000 g). Total thiosulfinates (Thio) were measured in supernatants with a spectrophotometric procedure [16]. One molecule of allicin reacts rapidly with two molecules of cysteine to form two molecules of S-allil mercapto-cysteine. The decrease in cysteine concentrations is measured by reaction with DTNB 5,5'-dithiobis (2-nitrobenzoic acid) at 412 nm. The results are expressed as μmol/g of fresh and unprocessed weight.
3.4. Glutathione Oxidized and Reduced
The plants’ organs were homogenized in ice-cold phosphate buffer 0.125 M (pH 7.4) containing 1 mM EDTA and then were added to an equal volume metaphosphoric acid (10%). Samples were centrifuged at low speed (2000 g) × 10 min at 0˚C. Reduced glutathione (GSH) and oxidized glutathione (GSSG) were quantified in supernatant by microassay procedure [17]. The results were expressed as nmol/mg protein.
3.5. Glutathione Reductase Activity
The organs of the plant were homogenized in cold 0.25 M sucrose in 0.1 M phosphate buffer, pH 7.4. The homogenates were centrifuged at 40,000 g × 20 min at 4˚C, and the supernatants were used for the glutathione reductase (GR) assay [18]. The results were expressed as U/mg protein.
3.6. Glutathione Peroxidase Assay
The organs of the plants were homogenized using the same method as described for GR. The glutathione peroxidase activity (GPx) was evaluated by measuring the change in absorbance at 340 nm, at 37˚C, caused by the oxidation of NADPH [19]. Enzyme activity was expressed as U/mg of protein.
3.7. Catalase Activity
The organs of the plants were homogenized in ice-cold phosphate buffer (0.125 M, pH 7.4) containing 1 mM EDTA and then centrifuged at 4000 g for 15 min at 4˚C. Catalase (CAT) activity was determined by a microassay procedure [20]. The results were expressed as nmol/min/mg of protein.
3.8. Proteins Assay
Protein concentrations were determined by the Lowry method and the calibration curves were prepared with dry bovine serum albumin [21].
4. Statistical Analysis
Statistical analysis was performed with the SPSS version 19 software package (SPSS Inc, Chicago, IL, USA). Levene’s test was used to assess if the groups have equal variances. One-way analysis of variance (ANOVA) was utilized to evaluate differences among the groups.
The Tukey’s HSD test was mostly used for multiple comparisons when the assumption of homoscedasticity was respected, while Welch test and Games-Howell post hoc test were used when the assumption of homoscedasticity was violated.
The values of p < 0.05 were considered significant.
The correlations among variables were established using Spearman’s rho correlation coefficients.
5. Results
The activities of antioxidant enzymes (CAT, GPx and GR) and the relative levels of GSH, GSSG, Thio, and AA (expressed as mean ± standard error) in the bulb and leaves of Allium sativum and in different fresh organs (bulbs, bulblets, flowers bulblets, leaves and flowers) of Allium roseum are reported in Table 1 and Table 2.
The data shows that Allium roseum possess effective antioxidant properties similar to garlic, however the values of enzymatic activities and the levels of non-enzymatic parameter are different in the various organs of the plant.
The highest activity of GPx (Table 1) is observed in the bulbs of Allium roseum when compared with the garlic’s bulbs (38.31 ± 1.289 U/mg protein and 3.58 ± 1.502 U/mg protein respectively, p < 0.001). Similarly, GPx activity is greater in the bulbs (38.31 ± 1.289 U/mg protein) than the flowers (0.03 ± 0.003 U/mg protein, p < 0.01), the bulblets (18.00 ± 0.731 U/mg protein, p < 0.01) and flowers bulblets (1.74 ± 0.131 U/mg protein, p < 0.01) of Allium roseum.
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Table 1. Antioxidant enzymes activity in different fresh organs (bulbs, bulblets, flowers bulblets, leaves and flowers) of Allium roseum and bulbs, leaves of Allium sativum.
The values are expressed as mean ± standard error. ap < 0.05; aap < 0.01; aaap < 0.001 compared with Allium sativum bulbs. bp < 0.05; bbp < 0.01; bbbp < 0.001 compared with Allium roseum bulbs.
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Table 2. Non-enzymatic antioxidants in different fresh organs (bulbs, bulblets, flowers bulblets, leaves and flowers) of Allium roseum and bulbs, leaves of Allium sativum.
The values are expressed as mean ± standard error. ap < 0.05; aap < 0.01; aaap < 0.001 compared with Allium sativum bulbs. bp < 0.05; bbp < 0.01; bbbp < 0.001 compared with Allium roseum bulbs.
The highest activity of CAT (Table 1) is observed in the bulbs of Allium roseum (645.75 ± 51.993 nmol/min/mg protein) when compared with flowers (65.49 ± 2.133 nmol/min/mg protein p < 0.05), bulblets (99.41 ± 5.776 nmol/min/mg protein p < 0.05) and flowers bulblets (100.16 ± 2.517 nmol/min/mg protein p < 0.05) of Allium roseum. No statistical significance is observed when activity of CAT in the bulbs of Allium roseum was compared to garlic bulbs.
It is remarkable that the high activity for GR observed in the bulbs of Allium roseum (4.40 ± 0.151, p < 0.01) is greater than bulbs of Allium sativum (Table 1). Finally, Allium roseum shows the GR activity greater in the bulbs (4.40 ± 0.151 U/mg protein) than leaves (1.07 ± 0.019 U/mg protein, p < 0.01), flowers (1.05 ± 0.013 U/mg protein, p < 0.01), bulblets (1.61 ± 0.018 U/mg protein, p < 0.01) and flowers bulblets (1.22 ± 0.078 U/mg protein, p < 0.001).
The highest levels of GSH (Table 2) are showed in the bulbs of Allium roseum (9.80 ± 0.061 nmol/mg protein, p < 0.05) when compared to bulbs of Allium sativum (4.23 ± 0.445 nmol/mg protein).
However, GSH levels are greater in the bulbs of Allium roseum (p < 0.001) when compared to leaves (1.68 ± 0.091 nmol/mg protein) flowers (2.15 ± 0.029 nmol/mg protein), bulbets (0.73 ± 0.038 nmol/mg protein) and flowers bulblets (2.52 ± 0.025 nmol/mg protein). The levels of GSSG show the same trend of reduced form. The highest levels of GSSG are found in the bulbs of Allium roseum (3.04 ± 0.121 nmol/mg protein; p < 0.05) when compared to bulbs of Allium sativum (Table 2). GSSG levels are greater in the bulbs of Allium roseum when compared to leaves (0.71 ± 0.028 nmol/mg protein), flowers (1.57 ± 0.096 nmol/mg protein), bulblets (0.33 ± 0.001 nmol/mg protein) (p < 0.001 respectively) and flowers bulblets (1.51 ± 0.029 nmol/mg protein, p < 0.05).
Different results are found for Thio levels (Table 2). The highest levels of Thio are found in the flowers bulblets of Allium roseum (35.46 ± 0.976, p < 0.01) when compared to bulbs of Allium sativum (14.25 ± 1.158), or when compared to bulbs (0.65 ± 0.168 μmol/g of fresh weight, p < 0.01) of Allium roseum. However, leaves (0.75 ± 0.289 μmol/g of fresh weight), flowers (18.87 ± 1.216 μmol/g of fresh weight), and bulblets (3.04 ± 0.093 μmol/g of fresh weight), (p < 0.01 respectively) of Allium roseum showed higher levels of Thio respect to Allium roseum bulbs. Moreover, the highest levels of Thio are shown in the bulbs of Allium roseum when compared to bulbs of Allium sativum (p < 0.05).
An interesting result was noted with AA levels. The highest content of AA (Table 2) is in flowers of Allium roseum (1.94 ± 0.047 μmol/g of fresh weight) when compared to bulbs of Allium sativum (0.23 ± 0.003, p < 0.01) or when compared to bulbs (0.28 ± 0.002 μmol/g of fresh weight, p < 0.01) of Allium roseum. Furthermore, the leaves (1.25 ± 0.048 μmol/g of fresh weight, p < 0.05), bulblets (0.79 ± 0.021 μmol/g of fresh weight) and flowers bulblets (0.45 ± 0.006 μmol/g of fresh weight), (p < 0.01 respectively) of Allium roseum also have a higher AA content than the bulbs of Allium roseum. However, the highest levels of AA are shown in the bulbs of Allium roseum when compared to bulbs of Allium sativum (p < 0.01).
Table 3 shows R and Q content in different organs of Allium species investigated. We found that the flavonols content (µg/g of fresh weight) was significantly higher in the flowers, where R was fifty times higher than Q (272.68 ± 28.083 and 4.13 ± 0.261 respectively, p < 0.001).
Allium roseum shows R content greater in the flowers (272.68 ± 28.083) than bulbs (3.89 ± 0.412, p < 0.01). Moreover, the content of R is significantly higher in the bulbs of Allium roseum than bulbs of Allium sativum (3.89 ± 0.412 and 3.55 ± 0.819 respectively; p < 0.05).
Also the content of Q is significantly higher (p < 0.001) in the flowers of Allium roseum (4.13 ± 0.261) than bulbs of Allium sativum (1.39 ± 0.140) or the other organs of Allium roseum. No significant difference was observed between Q levels in the other organs investigated of Allium roseum respect to bulbs of Allium sativum.
Moreover, by evaluating the antioxidant content in the leaves of Allium sativum with respect to the bulbs of Allium sativum, it should be noted that the content of CAT, GR, GSH, AA, R is significantly higher in the leaves (Tables 1-3).
Finally, in Table 4 the correlation between the levels of AA, flavonols (R and Q), Thio, GSH, GSSG and the antioxidant enzymatic activities (GR, GPx and CAT) in Allium species are reported. A significantly negative correlation was observed between the levels of AA and CAT, GR, GSH and GSSG, while the flavonoid content is positively correlated with the levels of non-enzymatic antioxidants and negatively correlated with the activity of antioxidant enzymes.
6. Discussion
In this study we observed a high content of antioxidants in every fresh organ of Allium roseum that could play a fundamental role in the nutritional and therapeutic field.
There is no research or study comparing the enzymatic and non-enzymatic antioxidant defence system of fresh organs of Allium sativum and Allium roseum.
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Table 3. Rutin and quercetin content in various fresh organs (bulbs, bulblets, flowers bulblets, leaves and flowers) of Allium roseum and bulbs, leaves of Allium sativum.
The values are expressed as µg/g of fresh weight (± standard error). ap < 0.05; aap < 0.01; aaap < 0.001 compared with Allium sativum bulbs
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Table 4. Correlations (rho Spearman’s coefficient) between flavonols, thiosulfinates and antioxidant capacities in Allium species.
Legend: Thio (thiosulfinates μmol/g of fresh); AA (ascorbic acid μmol/ml); GPx (glutathione peroxidase activity U/mg protein); CAT (catalase activity nmol/min/mg protein); GR (glutathione reductase activity U/mg Protein); GSH (glutathione reduced form nmol/mg protein); GSSG (glutathione oxidized form nmol/mg protein); R (rutin µg/g of fresh weight); Q (quercetin µg/g of fresh weight) (*p < 0.05; **p < 0.01; ***p < 0.001).
Moreover, this is the first time that flavonols were measured in accordance with antioxidant activity. Current evidence indicates that consumption of fruit and vegetables decreases the incidence of the most prominent diseases in developed countries [22]. There is an increased importance in identifying dietary plant compounds or spices as a source of natural antioxidants [23].
Our data shows that the bulbs of Allium roseum are rich in antioxidant substances so that they could be used in the medical field in the same way that bulbs of Allium sativum are already being used. Similarly to garlic, the bulbs of Allium roseum have the highest levels of GSH, GSSG and Thio revealing that also Allium roseum is rich in sulphur compounds, which are also the source of many health-promoting effects. The high levels of Thio in the bulbs, leaves and flowers bulblets of Allium roseum suggest also its possible use as a source of sulphur species. Certainly the presence of many reactive sulphur species are related to interesting biological activity in vivo, as antimicrobial, fungicidal, antiplatelet or anticancer activity [6] [24] [25]. In the plants sulphur compounds have many roles similar to those observed in mammalian cells [26]. The high level of AA in the flowers and others organs of the Allium roseum suggests that ascorbic acid plays an essential role in the plant’s antioxidant properties. In addition, AA is also related with cell division in plants and as a regulator for cellular processes including cell wall metabolism [27]. GPx, GR and CAT plays a key role in the response to oxidative stress by maintaining the functions like an antioxidant that scavenges reactive oxygen species, such as hydrogen peroxide and superoxide [28]. The higher activity of GPx and GR in Allium roseum compared to Allium sativum strengthens the antioxidant capacity of the Allium roseum bulbs. R is a flavonoid that is widely found in fruits and vegetables and the high levels observed bulbs, flowers bulblets and especially in flowers of Allium roseum make it a potential new sources for this bioactive compound with therapeutic and nutritive purposes [29]. Some studies investigate the possible uses of Allium flowers for their nutritional and health properties [30] [31]. Higher levels of AA, GSH, R and the elevated activity of GR and CAT were found in the garlic leaves, in comparison to the bulbs, and this provides new information for Allium sativum, particularly because the leaves are less used and investigated. Recently, interest in the wild Allium species has grown and these results provide new information for the Allium roseum, a wild-growing Allium species, which is less studied and the references to nutritional and medical applications are rarely given [29] [32] [33] [34].
7. Conclusions
Results suggest that the investigated Allium species can serve as a cost-effective source of high-value antioxidant nutraceuticals for protection against oxidative stress-related disorders.
Allium roseum displays antioxidant abilities in all investigated plant organs, especially in the bulbs. They are the organs with higher enzyme activity compared to the other organs of the plant. Moreover, the content of AA, GSH, GSSG, Thio and R, is important factors in determining the antioxidant activity of this plant.
Our research indicates that increased consumption of these garlic species can provide health benefits and prevent diseases associated with oxidative stress. Moreover, because of their described properties, all investigated plant organs can be used as new ingredients to improve diversity in the modern diet as wells as in the industries for manufacturing products with antioxidant activity.
Thus, the presented results indicated that Allium roseum exhibited antioxidant ability in all investigated plant organs especially in the bulbs and therefore bulbs could be used in the human diet as a source of natural antioxidants. Finally, all investigated Allium roseum organs could be used also in the pharmaceutical and cosmetic industry for manufacturing products with potent oxygen radical scavenger activity.
Acknowledgements
The present research was supported by funds of the University of Siena.