Hepatoprotective Effect of Guava (Psidium guajava L.) Leaf Extracts on Ethanol-Induced Injury on Clone 9 Rat Liver Cells
Hung-Hui Chen, Po-Hua Wu, Diana Lo, Yun-Chieh Pan, Ming-Chang Wu
DOI: 10.4236/fns.2011.29133   PDF    HTML   XML   7,122 Downloads   14,728 Views   Citations


Guava (Psidium guajava L.), a tropical fruit, belongs to Myrtaceae family. Leaves and fruits of guava have been reported to have an anti-diarrheal, hypoglycemic, lipid lowering, anti-bacterial in addition to antioxidant activities. The aim of this study was to investigate several guava leaf extract cytotoxic effects on healthy clone 9 liver cells and its hepatoprotective effects on ethanol-induced heap-toxicity. It was discovered that when the clone 9 liver cells were treated with guava (Psidium guajava Linn.) extracts for 24 hours, there was no retardation of growth as well as when ethanol and acetone extracts at low concentrations 100 μg/mL and 50 μg/mL were administered however cytototoxic effects were detected at higher concentrations. Water and hot water extracts in concentrations lower than or equal to 500 μg/mL revealed no cytotoxic effects. Injury induction to healthy clone 9 liver cells using 5% alcohol concentration for 30 minutes revealed the hepatoprotective properties of guava (Psidium guajava Linn.) extracts. This was significant in concentrations of 100 μg/mL or lower for ethanol and all concentrations for hot water extracts. Hot water extracts showed higher hepatoprotective and lower cytotoxic properties than other extracts.

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H. Chen, P. Wu, D. Lo, Y. Pan and M. Wu, "Hepatoprotective Effect of Guava (Psidium guajava L.) Leaf Extracts on Ethanol-Induced Injury on Clone 9 Rat Liver Cells," Food and Nutrition Sciences, Vol. 2 No. 9, 2011, pp. 983-988. doi: 10.4236/fns.2011.29133.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] K. A. N. Messingham, D. E. Faunce and E. J. Kovacs, “Alcohol, Injury, and Cellular Immunity,” Alcohol, Vol. 28, No. 3, 2002, pp. 137-149. doi:10.1016/S0741-8329(02)00278-1
[2] C. S. Lieber, “Alcoholic Fatty Liver: Its Pathogenesis and Mechanism of Progression to Inflammation and Fibrosis,” Alcohol, Vol. 34, No. 1, 2004, pp. 9-19. doi:10.1016/j.alcohol.2004.07.008
[3] S. Ramaiah, C. Rivera, and G. Arteel, “Early-Phase Alcoholic Liver Disease: An Update on Animal Models, Pathology, and Pathogenesis,” International Journal of Toxicology, Vol. 23, No. 4, 2004, pp. 217-231. doi:10.1080/10915810490502069
[4] R. N. M. MacSween and A. D. Burt, “Histologic Spectrum of Alcoholic Liver Disease,” Seminars Liver Disease, Vol. 6, No. 3, 1986, pp. 221-232. doi:10.1055/s-2008-1040605
[5] World Health Organization, “Causes of Death 2008: Data Sources and Methods,” World Health Organization, Geneva, 2010. http://www.who.int/healthinfo/global_burden_disease/cod_2008_sources_methods.pdf
[6] S. Shahani, “Evaluation of Hepatoprotective Efficacy of APCL-A Polyherbal Formulation in Vivo in Rats,” Indian Drugs, Vol. 36, 1999, pp. 628-631.
[7] R. M. Gutiérrez, S. Mitchell and R. V. Solis, “Psidium Guajava: A Review of Its Traditional Uses, Phytochemistry and Pharmacology,” Journal of Ethnopharmacology, Vol. 117, No. 1, 2008, pp. 1-27. doi:10.1016/j.jep.2008.01.025
[8] Y. Deguchi and K. Miyazaki, “Anti-Hyperglycemic and Anti-Hyperlipidemic Effects of Guava Leaf Extract,” Nutrition and Metabolism, Vol. 7, No. 1, 2010, pp. 1-9.
[9] I. B. Weinstein, N. Yamaguchi, J. M. Orenstein, R. Gebert and M. E. Kaighn, “Gene Expression and Carcinogenesis in Cultured Liver,” In: L. E. Gerschenson and E. B. Thompson, Eds., Academic Press, New York, 1975, pp. 441-459.
[10] N. J. Wu, “Hypoglycemic Effect of Guava (Psidium guajava Linn.) Leaf Extracts in Streptozotocin-Nicotinamide Induced Type 2 Diabetic Rat,” Ph.D. Thesis, National Taiwan University, Taipei, 2007.
[11] R. Sandhir and K. D. Gill, “Hepatoprotective Effects of Liv-52 on Ethanol Induced Liver Damage in Rats,” Indian Journal of Experimental Biology, Vol. 37, 1999, pp. 762-766.
[12] D. S. Pratt and M. M. Kaplan, “Evaluation of Abnormal Liver-Enzyme Result in Asymptomatic Patients,” The New England Journal of Medicine, Vol. 342, 2000, pp. 1266-1271. doi:10.1056/NEJM200004273421707
[13] L. V. Crowley, “The Reitman-Frankel Colorimetric Transminase Produced in Suspected Myocardial Infarction,” St. Mary’s Hospital, Minnesota, 1967.
[14] H. Y. Chen and G. C. Yen., “Antioxidant Activity and Free Radical-Scavenging Capacity of Extracts from Guava (Psidium guajava L.) Leaves,” Food Chemistry, Vol. 101, No. 2, 2006, pp. 686-694. doi:10.1016/j.foodchem.2006.02.047

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