Comparison of Two Parasitological Tests and Post Mortem Examination to Detect Prevalence of Bovine Fasciolosis at Ambo Town Municipal Abattoir, West Shewa Zone, Ethiopia

Diriba Taddese Legesse; Feyera Gemeda

doi:10.4236/oalib.1101719

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Open Access Library Journal > Vol.2 No.8, August 2015

Comparison of Two Parasitological Tests and Post Mortem Examination to Detect Prevalence of Bovine Fasciolosis at Ambo Town Municipal Abattoir, West Shewa Zone, Ethiopia ()

Diriba Taddese Legesse, Feyera Gemeda

School of Veterinary Medicine, Jimma University, Jimma, Ethiopia.

DOI: 10.4236/oalib.1101719 PDF HTML XML 829 Downloads 1,138 Views

Abstract

A cross sectional study was conducted from March 5, 2013 to June 28, 2013 in Ambo town municipal abattoir to assess prevalence of bovine fasciolosis and compare tests used for bovine fasciolosis. Direct smear, sedimentation and post mortem examination were the tests used for comparison. Post mortem examination was considered as gold standard test. Data were collected by using primary data at the abattoir; Kappa (K) and percentage were utilized to assess agreement of tests and prevalence, respectively. A total of 60 fecal samples were collected from cattle brought to the abattoir for direct smear and sedimentation techniques. Adult Fasciola were detected from slaughtered cattle at the abattoir. An overall prevalence of 21.66% (13 of 60), 18.33% (11 of 60) and 13.3% (8 of 60) were obtained by post mortem examination, sedimentation and direct smear techniques, respectively. At species level, 69.23% (9 of 13 positive cases) Fasciola hepatica and 84.62% (11 of 13 positive cases) Fasciola gigantica prevalence were observed. From 13 positive samples, 7 (53.85%) were mixed. Sedimentation showed higher agreement with post mortem examination (K = 0.896) than direct smear with post mortem examination (K = 0.715). The specificity of direct smear, sedimentation and post mortem examination were 61.53%, 84.6% and 100%, respectively. In conclusion, presence high prevalence of mixed infestation indicated that presence of suitable ecology for both Fasciola species and sedimentation were considered as better test than direct smear. Therefore, control strategies and utilization of sedimentation technique to detect Fasciola egg for samples collected from live animals were recommended.

Keywords

Ambo, Bovine Fasciolosis, Direct Smear, Sedimentation, Prevalence, Post Mortem Examination

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Legesse, D. and Gemeda, F. (2015) Comparison of Two Parasitological Tests and Post Mortem Examination to Detect Prevalence of Bovine Fasciolosis at Ambo Town Municipal Abattoir, West Shewa Zone, Ethiopia. Open Access Library Journal, 2, 1-5. doi: 10.4236/oalib.1101719.

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1]	Troncy, P.M. (1989) Helminthes of Life Stock and Poultry in Tropical Veterinary Parasitology. CAB international, 63-77.
[2]	Jobre, Y. and Malone, J.B. (1998) A Geographical Information System Forecast Model for Strategic Control of Fasciolosis in Ethiopia. Veterinary Parasitology, 78, 103-127. http://dx.doi.org/10.1016/S0304-4017(98)00136-8
[3]	WHO (1995) Okewole (200). Control of Food Borne Trematode Infections. Technical Report Series 849, 61.
[4]	Grabber, M. and Dagne, P. (1978) Mollusques vectuers detrematodoseshumaineret Animals en Ethiopia. Revuede Medicine Veterinairedays Pays Tropicaux, 27, 207-322.
[5]	Behm, C.A. and Sangster, N.C. (1999) Pathology, Pathophysiology and Clinical Aspects. In: Dalton, J.P., Ed., Fasciolosis, CABI Publishing, Wallingford, 185-224.
[6]	http://www.pmean.com/definitions/kappa.htm
[7]	Putt, M., Shaw, M., Tylerl, W.J. and James, D. (1988) Veterinary Epidemiology and Economics of Africa: Manual for Use in the Design and Appraisal of Live Stock Health Policy. ILCA, Addis Ababa, 231.
[8]	Askale, G., Dereje, R., Hawa, D., Nigatu, A. and Tenaye, B. (2011) Prevalence of Bovine Fasciolisis in Ambo Twon.
[9]	Sisay, M. (2011) Prevalence of Small Ruminant Fasciolosis at Addis Ababa A Battoir.
[10]	Cornelissen, B.W.J., Gaasenbeek, P.H., Borgsteede, H.M., Holland, W.G. Harmsen, M.M. and Boersma, W.J. (2001) Early Immuno Diagnosis of Fasciolosis in Ruminants Using Recombinant Fasciola hepatica Cathepsin L-Like Protease. International Journal for Parasitology, 31, 728-737. http://dx.doi.org/10.1016/S0020-7519(01)00175-8
[11]	Richeter, E.A., Ogundipe, G.A.T., Adejinmi, J.O. and Olaniyan, A.O. (2000) Clinical Evaluation of Three Chemo Prophylactic Regimes against Ovine Helminthosis in a Fasciola endemin Farm in Ibadan, Nigeria. Israel Journal of Veterinary Medicine, 56, 15-28.
[12]	Urquhart, G.M., Armour, J.D., Duncan, J.L., Dunn, A.M. and Jennings, F.W. (1989) Veterinary Parasitology. Low Priced Ed., English Language Book Society Longman, Blackwell, 286.
[13]	Soulsby, E.J.L. (1982) Helminth, Arthropod and Protozoa of Domestic Animals. 7th Edition.