Transmission and Scanning Electron Microscopy of Contacts between Bacterial and Yeast Cells in Biofilms on Different Surfaces

Karlen Hovnanyan; Seda Marutyan; Astghik Pepoyan; Liparit Navasardyan; Armen Trchounian

doi:10.4236/oalib.1101492

Home > Journals > Biomedical & Life Sciences | Business & Economics > OALibJ

Open Access Library Journal > Vol.2 No.4, April 2015

Transmission and Scanning Electron Microscopy of Contacts between Bacterial and Yeast Cells in Biofilms on Different Surfaces ()

Karlen Hovnanyan^1*, Seda Marutyan², Astghik Pepoyan³, Liparit Navasardyan², Armen Trchounian⁴

¹Institute of Molecular Biology of NAS RA, Yerevan, Armenia.
²Department of Biochemistry, Yerevan State University, Yerevan, Armenia.
³Armenian State Agrarian University, Yerevan, Armenia.
⁴Department of Microbiology, Plants and Microbes Biotechnology, Yerevan State University, Yerevan, Armenia.

DOI: 10.4236/oalib.1101492 PDF HTML XML 746 Downloads 1,247 Views Citations

Abstract

The mechanism formation of colonies and biofilms of bacteria and yeasts are studied always of great interest. The aim of the presented work was transmission and scanning electron microscopic analysis contacts between cells of bacteria and yeast in biomofilms on natural structures and inorganic surface, as a result of formation of close contacts between a cellular wall, a fringe, cross-pieces, symplasts and cells of Escherichia coli, Shigella flexnerii Salmonella of typhi, Salmonella typhimurium and also some probiotic lactic acid on nutritious agar surfaces. Intercellular contacts in yeast biomofilms on plates of zirconium were scanning electron micro-scopic visualized by Candida guilliermondii.

Keywords

Biofilms, Intercellular Contacts, Bacteria, Yeast, Transmission and Scanning Electronic Microscopy, Morphology Image Analysis

Share and Cite:

Hovnanyan, K. , Marutyan, S. , Pepoyan, A. , Navasardyan, L. and Trchounian, A. (2015) Transmission and Scanning Electron Microscopy of Contacts between Bacterial and Yeast Cells in Biofilms on Different Surfaces. Open Access Library Journal, 2, 1-10. doi: 10.4236/oalib.1101492.

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1]	Mhatre, E., Monterrosa, R.G. and Kovacs, A.T. (2014) From Environmental Signals to Regulators: Modulation of Biofilm Development in Gram-Positive Bacteria. Journal of Basic Microbiology, 54, 616-632. http://dx.doi.org/10.1002/jobm.201400175
[2]	Romanova, Y.M. and Ginzburg, A.L. (2011) Bacterial Biofilms as a Natural Form of Existence of Bacteria in the Environment and the Host. Journal of Microbiology, 3, 100-110. (In Russian)
[3]	Watnick, P. and Kolter, R. (2000) Biofilm, City of Microbes. Journal of Bacteriology, 182, 2675-2679. http://dx.doi.org/10.1128/JB.182.10.2675-2679.2000
[4]	Valyshev, A.B., Valisheva, I.V. and Haidee, I.V. (2009) The Formation of Biofilms by Fecal Strains of Enterobacteria and Yeast Fungi of the Genus Candida. Zhurnal Mikrobiologii, Epidemiologii, i Immunobiologii, 4, 44-46. (In Russian)
[5]	Amano, A., Nakagawa, I. and Hamada, S. (1999) Studying Initial Phase of Biofilm Formation: Molecular Interaction of Host Proteins and Bacterial Surface Components. Methods in Enzymology, 310, 501-513. http://dx.doi.org/10.1016/S0076-6879(99)10038-7
[6]	Chambless, J.D., Hunt, S.M. and Stewart, P.S. (2006) A Three-Dimensional Computer Model of Four Hypothetical Mechanisms Protecting Biofilms from Antimicrobials. Applied and Environmental Microbiology, 72, 2005-2013. http://dx.doi.org/10.1016/S0076-6879(99)10038-7
[7]	Al-Fattani, M.A. and Douglas, L.J. (2004) Penetration of Candida Biofilms by Antifungal Agents. Antimicrobial Agents and Chemotherapy, 48, 3291-3297. http://dx.doi.org/10.1128/AAC.48.9.3291-3297.2004
[8]	Xu, K.D., McFeters, G.A. and Stewart, P.S. (2000) Biofilm Resistance to Antimicrobial Agents. Microbiology, 146, 547-549.
[9]	Cogan, N.G., Cortez, R. and Fauci, L. (2005) Modeling Physiological Resistance in Bacterial Biofilms. Bulletin of Mathematical Biology, 67, 831-853. http://dx.doi.org/10.1016/j.bulm.2004.11.001
[10]	Apresyan, L.S., Amirkhanyan, N.H., Grigoryan, R.M., Sarkisyan, N.K., et al. (2012) Study in Vitro of the Biocompatibility of New Porous NIZ Allow as Potential Biomaterial. New Armenian Medical Journal, 6, 20-25.
[11]	Costerton, J.W., Montanaro, L. and Arciola, C.R. (2005) Biofilm in Implant Infections: Its Production and Regulation. International Journal of Artificial Organs, 28, 1062-1068.
[12]	Pilhofer, M., Ladinsky, M.S., McDowall, A.W. and Jensen, G.J. (2010) Bacterial TEM: New Insights from Cryo-Microscopy. Methods in Cell Biology, 96, 21-45. http://dx.doi.org/10.1016/S0091-679X(10)96002-0
[13]	Hovnanyan, K.O., Davtyan, H.H., Trchounian, A. and Pryatkin, N.S. (2009) Electron Microscopy and 3D Nano-Organization of Virus-Like and Surface Structure of Entamoeba, Candida and Escherichia Species. MC-2009, Graze, 2009, 333-334.
[14]	Hovnanyan, K.O. and Trchounian, A. (2009) Cell Wall and Cytoplasmic Membrane Structures of Some Bacteria: Novel Data and Role in Pathology. In: Trchounian, A.A., Ed., Bacterial Membranes. Ultrastructure, Bioelectrochemistry, Bioenergetics and Biophysics, Research Signpost, Kerala, 1-23.
[15]	Luft, J.H. (1971) Ruthenium Red and Violet. II. Fine Structural Localization in Animal Tissues. The Anatomical Record, 171, 369-415. http://dx.doi.org/10.1002/ar.1091710303
[16]	Bayer, M.E. and Bayer, M.H. (1986) Effects of Bacteriophage FD Infection on Escherichia coli HB11 Envelope: A Morphological and Biochemical Study. Journal of Virology, 57, 258-266.
[17]	Torjian, I.Kh. and Hovnanyan, K.O. (1982) Influence of Subbraking Concentration of Antibiotics with Various Actions Mechanism a on Submicroscopic Structure of Salmonellas. Journal of Experimental & Clinical Medicine, 22, 418-421. (In Russian)
[18]	Kuklin, V.V., Emeliyanova, L.K., Zhdanov, V.G. and Yustratov, L.S. (1983) Use of a Method of Merge of Protoplast in Producer Selection of Streptomycin Antibiotic Grizin. Antibiotics, 10, 883-888. (In Russian)
[19]	Flemming, H.C. and Wingender, J. (2010) The Biofilm Matrix. Nature Reviews Microbiology, 8, 623-633. http://dx.doi.org/10.1038/nrmicro2415
[20]	Rehm, B.H.A. (2010) Bacterial Polymers: Biosynthesis, Modifications and Applications. Nature Reviews Microbiology, 8, 578-592. http://dx.doi.org/10.1038/nrmicro2354
[21]	Hovnanyan, K. (2014) Model of Sterile Intestine of Mice with Normal Microfloras: Histological and Ultrastructural Visualization Formation Kyst Organ-Like Intestine for the Study of Interaction of Pathogen Agents with the Host Sterile Intestine. Open Access Library Journal, 1, 1-6. http://dx.doi.org/10.4236/oalib.1100431
[22]	Brudastov, Yu.A., Gricenko, V.A., Zhurlov, O.S. and Chertkov, K.L. (1997) The Characteristic of Hydrophobic Properties of Bacteria at Their Interaction with Whey of Blood. Journal of Hygiene, Epidemiology, Microbiology, and Immunology, 4, 73-74. (In Russian)
[23]	Hambarcumyan, A.Dz. (2002) Lactobacillus in Preventive Maintenance Intrahospital Infections. “Vaan” Press, Yerevan, 248 p. (In Russian)
[24]	Glushanova, H.A., Blinov, A.I. and Bahaeev, V.V. (2004) Antagonism of Probiotic Lactobacilli. Journal of Epidemiology Infection Diseases, 6, 37-39.
[25]	Didenko, L.V., Avtandilov, G.A., Shevlyagina, N.V. and Smirnova, T.A. (2012) Biodestruction of Polyurethane by Staphylococcus aureus (an Investigation by SEM, TEM and FIB). In: Méndez-Vilas, A., Ed., Current Microscopy Contributions to Advances in Science and Technology, Microscopy Series 5, 1, 323-334.
[26]	Kariminiaae-Hamedaani, H.R., Kanda, K. and Kato, F. (2003) Wastewater Treatment with Bacteria Immobilized onto a Ceramic Carrier in an Aeration System. Journal of Bioscience and Bioengineering, 95, 128-132. http://dx.doi.org/10.1016/S1389-1723(03)80117-2
[27]	Khatoon, N., Naz, I. and Ishtiaq, A.M. (2014) Bacterial Succession and Degradative Changes by Biofilm on Plastic Medium for Wastewater Treatment. Journal of Basic Microbiology, 54, 739-749. http://dx.doi.org/10.1002/jobm.201300162