UV Spectrophotometric Assay Method for the Determination of Fluconazole Capsules

DOI: 10.4236/oalib.1101468   PDF   HTML   XML   1,766 Downloads   3,268 Views   Citations


A very simple, accurate, rapid and economical UV spectrophotometric method was developed for the estimation of fluconazole. The UV method permits a simple, rapid and economical quantitation of fluconazole in capsule dosage form only. The maximum absorbance of fluconazole was found to be 210 nm. Fluconazole, which is available in Karachi in different brands within the pharmacies, was collected and used for this assay method. The results showed that the assay results of the two brands were in range. This study would turn out to be very beneficial and with this simple and accurate method, one can easily determine fluconazole.

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Naveed, S. and Nafees, M. (2015) UV Spectrophotometric Assay Method for the Determination of Fluconazole Capsules. Open Access Library Journal, 2, 1-5. doi: 10.4236/oalib.1101468.

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The authors declare no conflicts of interest.


[1] Merck & Co., Inc. (2006) The Merck Index. 14th Edition, Merck & Co., Inc., Whitehouse Station, Monograph No. 0004122.
[2] USP Convention (2012) United States Pharmacopeia, USP35, National formulary-32. USP Convention, Rockville, Fluconazole, 3204-3205.
[3] Council of Europe (2012) European Pharmacopoeia, EDQM. Edition 7.6, Council of Europe, Strasbourg, 4936-4937.
[4] Stationery Office (2009) Pharmacopoeia. Her Majesty’s, Stationery Office, London, 1&2 Monograph No. 2287.
[5] Martindale (1996) The Extra Pharmacopoeia. Reynolds, J.E.F., Ed., 31st Edition, Royal Pharmaceutical Society, London, 404-406.
[6] Saurabh, P., Preeti, P., Shaifali, D., Udisha, C. and Awani, K.R. (2012) Facile Derivative UV Spectroscopy Method: Simultaneous Estimation of Tinidazole and Fluconazole in Combined Tablet Dosage Form. Thai Journal of Pharmaceutical Sciences, 36, 55-62.
[7] Porta, V., Chang, K.H. and Storpirtis, S. (2005) Evaluation of the Bioequivalence of Capsules Containing 150 mg of Fluconazole. International Journal of Pharmaceutics, 288, 81-86.
[8] Joseph, T.D., Robert, L.T., Gary, C.Y., Gary, R.M., Barbara, G.W. and Michael, P. (2005) Pharmacotherapy: A Pathophysiologic Approach. 6th Edition, McGraw Hill, London.
[9] Lacy, C.F., Armstrong, L.L., Goldman, M.P. and Lance, L.L. (2006) Drug Information Handbook. 15th Edition, LEXI-COMP, Ohio.
[10] Kai, B.L., Gabriel, O.K.L., Yvonne, T.F.T. and Khiang, K.O.K. (2012) Development and Application of Simple HPLC-UV Method for Fluconazole Quantification in Human Plasma. International Journal of Pharmacy and Pharmaceutical Sciences, 4, 107-111.
[11] Abdel-Aleem, A.A., Lotfy, H.M. and Monir, H.H. (2012) Stability-Indicating High Performance Liquid Chromatographic Determination of Fluconazole in the Presence of Its Oxidative Degradation-Kinetic and Stress Study. International Research Journal of Pharmaceuticals, 2, 6-12.
[12] Gondalia, R.P., Patel, D.P. and Savaliya, P.J. (2010) Development and Validation of UV Spectrophotometric Method for Estimation of Fluconazole in Soft Gelatin Capsule. International Journal of Pharmacy and Technology, 2, 938-944.
[13] Shewiyo, D.H., Kaale, E., Risha, P.G., Dejaegher, B., Smeyers-Verbeke, J. and Vander, H.Y. (2012) HPTLC Methods to Assay Active Ingredients in Pharmaceutical Formulations: A Review of the Method Development and Validation Steps. Journal of Pharmaceutical and Biomedical Analysis, 66, 11-23.
[14] Yanamandra, R., Chaudhary, A., Bandaru, S.R., Sastry, C.S.P., Patro, B., Murthy, Y.L.N. and Ramaiah, P.A. (2011) Development of a RP-UPLC Method for the Simultaneous Analysis of Secnidazole, Fluconazole, and Azithromycin: Application in Pharmaceuticals and Human Serum. International Journal of PharmTech Research, 3, 1198-1207.
[15] Vinay, K.B., Revanasiddappa, H.D., Xavier, C.M., Ramesh, P.J. and Raghu, M.S. (2012) A Stability Indicating UPLC Method for the Determination of Tramadol Hydrochloride: Application to Pharmaceutical Analysis. Chromatography Research International, 2012, Article ID: 870951.
[16] Lotfy, H.M., Abdel-Aleem, A.A. and Monir, H.H. (2013) Stability-Indicating High Performance Liquid Chromatographic Determination of Fluconazole in the Presence of Its Oxidative Degradation Product—Kinetic and Stress Study. Journal of Liquid Chromatography & Related Technologies, 36, 1013-1029.
[17] Singh, A., Sharma, P.K. and Majumdar, D.K. (2014) Development and Validation of New HPLC-Methods for Estimation of Fluconazole in Different Simulated Biological Fluids: A Comparative Study. Journal of Liquid Chromatography & Related Technologies, 37, 594-607.
[18] Sadasivudu, P., Shastri, N. and Sadanandam, M. (2009) Development and Validation of RP-HPLC and UV Methods of Analysis for Fluconazole in Pharmaceutical Solid Dosage Forms. International Journal of ChemTech Research, 1, 1131-1136.
[19] Zhang, S.M., Mada, S.R., Torch, M., Goyal, R.K. and Venkataramanan, R. (2008) Development and Validation of a High-Performance Liquid Chromatographic Assay for the Determination of Fluconazole in Human Whole Blood Using Solid Phase Extraction. Therapeutic Drug Monitoring, 30, 314-319.
[20] Alizadeh, N. and Rezakhani, Z. (2012) Extractive Spectrophotometric Determination of Ketoconazole, Clotrimazole and Fluconazole by Ion-Pair Complex Formation with Bromothymol Blue and Picric Acid. Journal of the Chilean Chemical Society, 57, 1104-1108.
[21] Singh, A., Sharma, P.K. and Majumdar, D.K. (2011) Development and Validation of Different UV-Spectrophotometric Methods for the Estimation of Fluconazole in Bulk and in Solid Dosage Form. Indian Journal of Chemical Technology, 18, 357-362.
[22] Naveed, S. and Qamar, F. (2014) Simple UV Spectrophotometric Assay of Metronidazole. Open Access Library Journal, 1, 1-4.
[23] Naveed, S., Ashraf, Z. and Mukhtar, T. (2014) Assay of Different Brands of Cefadroxil by Using Spectrophotometric Method. Mintage Journal of Medical and Pharmaceutical Sciences, 3, 12-14.
[24] Naveed, S., Shah, S.N., Qamar, F., Waheed, N. and Nazeer, S. (2014) Simple UV Spectrophotometric Assay of New Formulation Gentamycin. Journal of Applied Pharmacology, 6, 407-410.
[25] Naveed, S. and Zafar, F. (2014) UV Spectrophotometric Assay of Famotidine Formulations. American Journal of Pharmacy and Pharmacology, 1, 28-31.

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