Protective Effect of Ischemic Preconditioning on Hepatic Ischemia-Reperfusion Injury in Rats


Aim: Ischemic preconditioning (IPC) protects tissue against ischemia and reperfusion (I/R) injury. The aim of this study was to examine the impact of IPC on protection of hepatocytes after prolonged I/R injury. Methods: Twenty-four Wistar rats were randomly divided into ischemic preconditioning group (IP), ischemia/reperfusion group (IR) and sham operation group (SO). A model of partial liver ischemia/reperfusion was used, in which rats were subjected to liver ischemia for 90 min prior to reperfusion. The animals in the IP group underwent ischemic preconditioning for 10 min prior to the ischemia/reperfusion challenge. After 3 h of reperfusion, serum and liver tissue in each group were collected to detect the level of serum ALT, AST, HA, MDA and liver histopathology. Results: Compared with IR group, IP group showed a significantly lower ALT, AST, HA and MDA level in 3 h. Proliferation index (PI) was significantly increased in IP group compared with IR group. Conclusion: Ischemic preconditioning can protect liver cells against ischemia/reperfusion injury, which may be related to cell proliferation during early ischemic reperfusion.

Share and Cite:

Hashimoto, N. (2015) Protective Effect of Ischemic Preconditioning on Hepatic Ischemia-Reperfusion Injury in Rats. Open Access Library Journal, 2, 1-5. doi: 10.4236/oalib.1101306.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] Montalvo, E.E., Escalante, T., Pina, E., et al. (2008) Factors in the Pathophysiology of the Liver Ischemia-Reperfusion Injury. Journal of Surgical Research, 147, 153-159.
[2] DeOliveira, M.L., Graf, R. and Clavien, P.A. (2008) Ischemic Preconditioning: Promises from the Laboratory to Patients-Sustained or Disillusioned? American Journal of Transplantation, 8, 489-491.
[3] Mehendale, H.M., Roth, R.A., Gandolfi, A.J., et al. (1994) Novel Mechanisms in Chemically Induced Hepatotoxicity. FASEB Journal, 8, 1285-1295.
[4] Cai, Z.W. and Mehendale, H.M. (1991) Protection from CC4 Toxicity by Prestimulation of Hepatocellular Regeneration in Partially Hepatectomized Gerbils. Biochemical Pharmacology, 42, 633-644.
[5] Peralta, C., Serafin, A., Fernandez-Zabalegui, L., Wu, Z.Y., et al. (2003) Liver Ischemic Preconditioning: A New Strategy for the Prevention of Ischemia-Reperfusion Injury. Transplantation Proceedings, 35, 1800-1802.
[6] Reinders, M.E., van Wagensveld, B.A., van Gulik, T.Y., et al. (1996) Hyaluronic Acid Uptake in the Assessment of Sinusoidal Endothelial Cell Damage after Cold Storage and Normothermic Reperfusion of Rat Livers. Transplant International, 9, 446-453.
[7] Cavalieri, B., Perrelli, M.G., Yang, J.J., et al. (2002) Ischemic Preconditioning Attenuates the Oxidant-Dependent Mechanisms of Reperfusion Cell Damage and Death in Rat Liver. Liver Transplantation, 8, 990-999.
[8] Peralta, C., Serafin, A., Fernandez-Zabalegui, L., et al. (2002) Ischemic Preconditioning: A Defense Mechanism against the Reactive Oxygen Species Generated after Hepatic Ischemia Reperfusion. Transplantation, 73, 1203-1211.
[9] Bravo, R. and Macdonald-Bravo, H. (1991) Existence of Two Populations of Cyclin/Proliferating Cell Nuclear Antigen during the Cell Cycle: Association with DNA Replication Sites. Journal of Cell Biology, 19, 29-33.
[10] Zhang, B.H., Gong, D.Z. and Mei, M.H. (1999) Protection of Regenerating Liver after Partial Hepatectomy from Carbon Tetrachloride Hepatotoxicity in Rats: Role of Hepatic Stimulator Substance. Journal of Gastroenterology and Hepatology, 14, 1010-1017.
[11] Schauer, R.J., Gerbes, A.L., Vonier, D., et al. (2003) Induction of Cellular Resistance against Kupffer Cell-Derived Oxidant Stress: A Novel Concept of Hepatoprotection by Ischemic Preconditioning. Hepatology, 37, 286-295.
[12] Cai, F.G., Xiao, J.S. and Ye, Q.F. (2006) Effects of Ischemic Preconditioning on Cyclin D1 Expression during Early Ischemic Reperfusion in Rats. World Journal of Gastroenterology, 12, 2936-2940.
[13] Carini, R., De Cesaris, M.G., Splendore, R., et al. (2001) Signal Pathway Involved in the Development of Hypoxic Preconditioning in Rat Hepatocytes. Hepatology, 33, 131-139.

Copyright © 2022 by authors and Scientific Research Publishing Inc.

Creative Commons License

This work and the related PDF file are licensed under a Creative Commons Attribution 4.0 International License.