Loop-Mediated Isothermal Amplification (LAMP) Assay for GMO on: Recent Progresses and Future Perspectives

DOI: 10.4236/oalib.1101264   PDF   HTML   XML   938 Downloads   1,631 Views   Citations


As more and more genetically modified (GM) crops are approved for commercialization and planting, safety issues of GM crops have become hot topics worldwide. For both regulatory and academic purposes, development of rapid, economic and effective on-site detection methods for GM components is indispensible. Up to now, the most effective and sensitive techniques used for GMO detection are based on polymerase chain reaction (PCR). PCR method needs expensive, heavy instruments and gel electrophoresis, therefore, it is commonly used in laboratory test, and unsuitable for on-site detection. Loop-mediated isothermal amplification (LAMP), an isothermal nucleic acid amplification technique, has been extensively used in many areas such as food safety and clinic diagnosis. Advantageous characteristics of LAMP, such as high specificity and sensitivity, simple operation, low cost, eye visualization, particularly free of special equipment, renders it with high potential to be used for GMO on-site detection. In this review, we summarized current status of the application of LAMP in GMO detection, and discussed possible improvements needed for its adaptability regarding to on-site GMO detection. Hopefully, the information present here would facilitate the practical risk assessment of GMO.

Share and Cite:

Li, R. , Wang, C. , Ji, L. , Zhao, X. , Liu, M. , Zhang, D. and Shi, J. (2015) Loop-Mediated Isothermal Amplification (LAMP) Assay for GMO on: Recent Progresses and Future Perspectives. Open Access Library Journal, 2, 1-8. doi: 10.4236/oalib.1101264.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] James, C. (2011) Global Status of Commercialized Biotech/GM Crops: 2011. Executive Summary. ISAAA Brief 43.
[2] Smythe, S., Kerr, W.A. and Davey, K.A. (2006) Closing Markets to Biotechnology: Does It Pose an Economic Risk If Markets Are Globalised? International Journal of Technology and Globalisation, 2, 377-389.
[3] Broeders, S.R.M., Keersmaecker, S.C.J. and Roosens, N.H.C. (2012) How to Deal with the Upcoming Challenges in GMO Detection in Food and Feed. BioMed Research International, 2012, Article ID: 402418.
[4] Bonfini, L. (2002) Review of GMO Detection and Quantification Techniques. Institute for Health and Consumer Protection. Food Products and Consumer Goods Unit, 1-74.
[5] Zhang, D.B. and Guo, J.C. (2011) The Development and Standardization of Testing Methods for Genetically Modified Organisms and Their Derived Products. Journal of Integrative Plant Biology, 53, 539-551.
[6] Suchitra, K. and Sher, A. (2013) Genetically Modified Crops: Detection Strategies and Biosafety Issues. Gene, 522, 123-132.
[7] Randhawa, G.J., Singh, M. and Morisset, D. (2013) Loop-Mediated Isothermal Amplification: Rapid Visual and Real-Time Methods for Detection of Genetically Modified Crops. Journal of Agricultural and Food Chemistry, 61, 11338-11346.
[8] James, C. (2013) Global Status of Commercialized Biotech/GM Crops: 2013; ISAAA Brief No. 46. International Service for the Acquisition Agri-Biotech Applications (ISAAA), Ithaca.
[9] Gruere, G.P., Carter, C.A. and Hossein, F. (2009) Explaining International Differences in Genetically Modified Food Labeling Policies. Review of International Economics, 17, 393-408.
[10] Arne, H.J. (2009) Testing for Genetically Modified Organisms (GMOs): Past, Present and Future Perspectives. Biotechnology Advances, 27, 1071-1082.
[11] Marmiroli, N., Maestri, E., Gullì, M., Malcevschi, A., Peano, C., Bordoni, R. and De Bellis, G. (2008) Methods for Detection of GMOs in Food and Feed. Analytical and Bioanalytical Chemistry, 392, 369-384.
[12] Arne, H.J., Marc, D.L. and Guy, V.D.E. (2006) Coherence between Legal Requirements and Approaches for Detection of Genetically Modified Organisms (GMOs) and Their Derived Products. Journal of Agricultural and Food Chemistry, 54, 2799-2809.
[13] Guan, X.Y., Guo, J.C., Shen, P., Yang, L.T. and Zhang, D.B. (2010) Visual and Rapid Detection of Two Genetically Modified Soybean Events Using Loop-Mediated Isothermal Amplification Method. Food Analytical Methods, 3, 313-320.
[14] Notomi, T., Okayama, H., Masubuchi, H., Yonekawa, T., Watanabe, K., Amino, N. and Hase, T. (2000) Loop-Mediated Isothermal Amplification of DNA. Nucleic Acids Research, 28, e63.
[15] Mori, Y. and Notomi, T. (2009) Loop-Mediated Isothermal Amplification (LAMP): A Rapid, Accurate, and Cost-Effective Diagnostic Method for Infectious Diseases. Journal of Infection and Chemotherapy, 15, 62-69.
[16] Fu, S.J., Qu, G.G., Guo, S.J., Ma, L., Zhang, N., Zhang, S.L., Gao, S.Y. and Shen, Z.Q. (2011) Applications of Loop-Mediated Isothermal DNA Amplification. Applied Biochemistry and Biotechnology, 163, 845-850.
[17] Mori, Y., Kanda, H. and Notomi, T. (2013) Loop-Mediated Isothermal Amplification (LAMP): Recent Progress in Research and Development. Journal of Infection and Chemotherapy, 19, 404-411.
[18] Francois, P., Tangomo, M., Hibbs, J., Bonetti, E.J., Boehme, C.C., Notomi, T., Perkins, M.D. and Schrenzel, J. (2011) Robustness of a Loop Mediated Isothermal Amplification Reaction for Diagnostic Applications. FEMS Immunology and Medical Microbiology, 62, 41-48.
[19] Kiddle, G., Hardinge, P., Buttigieg, N., Gandelman, O., Pereira, C., McElgunn, C., Rizzoli, M., Jackson, R., Appleton, N., Moore, C., Tisi, L. and Murray, J. (2012) GMO Detection Using a Bioluminescent Real Time Reporter (BART) of Loop Mediated Isothermal Amplification (LAMP) Suitable for Field Use. BMC Biotechnology, 12, 15.
[20] Tomita, N., Mori, Y., Kanda, H. and Notomi, T. (2008) Loop-Mediated Isothermal Amplification (LAMP) of Gene Sequences and Simple Visual Detection of Products. Nature Protocols, 3, 877-882.
[21] Asiello, P.J. and Baeumner, A.J. (2011) Miniaturized Isothermal Nucleic Acid Amplification: A Review. Lab on a Chip, 11, 1420-1430.
[22] Fukuta, S., Mizukami, Y., Ishida, A., Ueda, J., Hasegawa, M., Hayashi, I., Hashimoto, M. and Kanbe, M. (2004) Real-Time Loop-Mediated Isothermal Amplification for the CaMV-35S Promoter as a Screening Method for Genetically Modified Organisms. European Food Research and Technology, 218, 496-500.
[23] Lee, D., La, M.M., Allnutt, T. and Powell, W. (2009) Detection of Genetically Modified Organisms (GMOs) Using Isothermal Amplification of Target DNA Sequences. BMC Biotechnology, 9, 7.
[24] Chen, J., Huang, C., Zhang, X., Yu, R. and Wu, Z. (2011) Detection of Herbicide-Resistant Maize by Using Loop-Mediated Isothermal Amplification of the Pat Selectable Marker Gene. African Journal of Biotechnology, 10, 17055-17061.
[25] Li, Q., Fang, J., Liu, X., Xi, X., Li, M., Gong, Y. and Zhang, M. (2013) Loop-Mediated Isothermal Amplification (LAMP) Method for Rapid Detection of cry1Ab Gene in Transgenic Rice (Oryza sativa L.). European Food Research and Technology, 236, 589-598.
[26] Li, F.W., Yan, W., Long, L.K., Qi, X., Li, C.C. and Zhang, S.H. (2014) Development and Application of Loop-Mediated Isothermal Amplification Assays for Rapid Visual Detection of cry2Ab and cry3A Genes in Genetically-Modified Crops. International Journal of Molecular Sciences, 15, 15109-15121.
[27] Chen, L.L., Guo, J.C., Wang, Q.D., Kai, G.Y. and Yang, L.T. (2011) Development of the Visual LAMP Assays for Seven Genetically Modified Maize Events and Their Application in Practical Samples Analysis. Journal of Agricultural and Food Chemistry, 59, 5914-5918.
[28] Niloofar, R., Ali, H., Masoud, T. and Aboubakr, M. (2011) Rapid Identification of Transgenic Cotton (Gossypium hirsutum L.) Plants by Loop-Mediated Isothermal Amplification. Czech Journal of Genetics and Plant Breeding, 47, 140-148.
[29] Chen, X.Y., Wang, X.F., Jin, N., Zhou, Y., Huang, S.N., Miao, Q.M., Zhu, Q. and Xu, J.F. (2012) Endpoint Visual Detection of Three Genetically Modified Rice Events by Loop-Mediated Isothermal Amplification. International Journal of Molecular Sciences, 13, 14421-14433.
[30] Cheng, Y., Zhang, M.H., Hu, K., Sun, F.D., Tao, R., Gao, X.J. and Luan, F.X. (2013) Loop-Mediated Isothermal Amplification for the Event-Specific Detection of Wheat B73-6-1. Food Analytical Methods, 7, 500-505.
[31] Huang, X., Chen, L.L., Xu, J.M., Ji, H.F., Zhu, S.F. and Chen, H.J. (2014) Rapid Visual Detection of Phytase Gene in Genetically Modified Maize Using Loop-Mediated Isothermal Amplification Method. Food Chemistry, 156, 184-189.
[32] Zhang, M., Liu, Y.N., Chen, L.L., Quan, S., Jiang, S.M., Zhang, D.B. and Yang, L.T. (2013) One Simple DNA Extraction Device and Its Combination with Modified Visual Loop-Mediated Isothermal Amplification for Rapid On-Field Detection of Genetically Modified Organisms. Analytical Chemistry, 85, 75-82.

comments powered by Disqus

Copyright © 2020 by authors and Scientific Research Publishing Inc.

Creative Commons License

This work and the related PDF file are licensed under a Creative Commons Attribution 4.0 International License.