Chemical Characterization and in Vitro Antitumor Activity of the Essential Oils from the Leaves and Flowers of Callistemon viminalis
Christiane Maria de Oliveira1, Maria das Graças Cardoso1*, Marisa Ionta2, Marisi Gomes Soares3, Juliana de Andrade Santiago1, Guilherme Álvaro Ferreira da Silva2, Maria Luisa Teixeira1, Danúbia Aparecida de Carvalho Selvati Rezende1, Rafaela Vieira de Souza1, Luana Isac Soares1, David Lee Nelson4, Marcos Schleiden Sousa Carvalho5
1Department of Chemistry, Federal University of Lavras, Lavras, Brazil.
2Institute of Biomedical Sciences, Federal University of Alfenas, Alfenas, Brazil.
3Department of Chemistry, Federal University of Alfenas, Alfenas, Brazil.
4Federal University of Vales de Jequitinhonha e Mucuri, Diamantina, Brazil.
5Department of Agriculture, Federal University of Lavras, Lavras, Brazil.
DOI: 10.4236/ajps.2015.616268   PDF   HTML   XML   4,382 Downloads   5,255 Views   Citations

Abstract

The objective of this study was to characterize and verify the in vitro antitumor activity of essential oils (EOs) extracted from the leaves and flowers of Callistemon viminalis. The EOs were extracted by hydrodistillation using a modified Clevenger apparatus. The identification and quantification of constituents were performed on a gas chromatograph coupled to a mass spectrometer and a gas chromatograph with a flame ionization detector. The antitumoral activity was evaluated by a colorimetric assay (MTS) using different cell lines derived from human tumors (breast, lung, glioblastoma, and melanoma). The major constituents of the EOs of leaves and flowers were similar, only quantitative differences being observed. The compounds 1,8-cineole, α-pinene and α-terpineol were found in concentrations of 50.4%, 25.8% and 8.7% in the EOs obtained from the leaves and 48.8%, 24.5% and 3.9% in the EOs obtained from the flowers, respectively. The cytotoxic activity of the EOs was observed only in melanoma cultures (HT144). Cultures treated for 48 h with EOs from leaves and flowers (200 μg·mL-1) reduced the viability by 40% and 25%, respectively. Thus, the antiproliferative activity of the EO from leaves was more pronounced than the EO from flowers in cells derived from melanoma.

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de Oliveira, C. , Graças Cardoso, M. , Ionta, M. , Soares, M. , Andrade Santiago, J. , da Silva, G. , Teixeira, M. , Selvati Rezende, D. , de Souza, R. , Soares, L. , Nelson, D. and Sousa Carvalho, M. (2015) Chemical Characterization and in Vitro Antitumor Activity of the Essential Oils from the Leaves and Flowers of Callistemon viminalis. American Journal of Plant Sciences, 6, 2664-2671. doi: 10.4236/ajps.2015.616268.

Conflicts of Interest

The authors declare no conflicts of interest.

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